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Searching in 'World-2DPAGE Repository [0003]' for entry matching: P10896




World-2DPAGE Repository (0003):  P10896


P10896


General information about the entry
View entry in simple text format
Entry nameRCA_ARATH
Primary accession numberP10896
Secondary accession number(s) Q940T8
integrated into World-2DPAGE Repository (0003) on October 14, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on November 15, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic; Short=RA; Short=RuBisCO activase; Flags: Precursor;.
Gene nameName=RCA
OrderedLocusNames=At2g39730
ORFNames=T5I7.3
Annotated speciesArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
TaxonomyEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core; eudicotyledons; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
References
[1]   MAPPING ON GEL
PubMed=18656559; DOI=10.1016/j.jprot.2008.06.012; [NCBI, EBI, Israel, Japan]
Maldonado A.M., Echevarria-Zomeno S., Jean-Baptiste S., Hernandez M., Jorrin-Novo J.V.
''Evaluation of three different protocols of protein extraction for Arabidopsis thaliana leaf proteome analysis by two-dimensional electrophoresis''
J. Proteomics 71(4):461-472 (2008)
2D PAGE maps for identified proteins
How to interpret a protein

PHENOL_2 {Phenol 2}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
PHENOL_2
  map experimental info
 
PHENOL_2

MAP LOCATIONS:
pI=5.98; Mw=44770  [identification data]
pI=6.08; Mw=44460  [identification data]

IDENTIFICATION: SPOT 43: SeqCov=20%. Mascot (Protein score/CI%=157/100, Total ion score/CI%=95/100) [1]
SPOT 54: SeqCov=38%. Mascot (Protein score/CI%=366/100, Total ion score/CI%=216/100) [1].
MAPPING (identification):
SPOT 43: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 54: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].



TCA-ACETONE_3 {TCA-Acetone 3}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
TCA-ACETONE_3
  map experimental info
 
TCA-ACETONE_3

MAP LOCATIONS:
pI=5.98; Mw=44770  [identification data]
pI=6.08; Mw=44460  [identification data]

IDENTIFICATION: SPOT 43: SeqCov=20%. Mascot (Protein score/CI%=157/100, Total ion score/CI%=95/100) [1]
SPOT 54: SeqCov=38%. Mascot (Protein score/CI%=366/100, Total ion score/CI%=216/100) [1].
MAPPING (identification):
SPOT 43: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 54: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].



TCA-ACETONE-PHENOL_3 {TCA-Acetone-Phenol 3}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
TCA-ACETONE-PHENOL_3
  map experimental info
 
TCA-ACETONE-PHENOL_3

MAP LOCATIONS:
pI=5.98; Mw=44770  [identification data]
pI=6.08; Mw=44460  [identification data]

IDENTIFICATION: SPOT 43: SeqCov=20%. Mascot (Protein score/CI%=157/100, Total ion score/CI%=95/100) [1]
SPOT 54: SeqCov=38%. Mascot (Protein score/CI%=366/100, Total ion score/CI%=216/100) [1].
MAPPING (identification):
SPOT 43: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 54: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jesus V. Jorrin-Novo, University of Cordoba, Spain
Cross-references
SWISS-2DPAGEP10896; RCA_ARATH.
UniProtKB/Swiss-ProtP10896; RCA_ARATH.
World-2DPAGE RepositoryP10896; RCA_ARATH.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRCA_ARATH
Primary accession numberP10896
Secondary accession number(s) Q39197 Q39198 Q940T8
Sequence was last modified on December 1, 2000 (version 2)
Annotations were last modified on October 19, 2011 (version 115)
Name and origin of the protein
DescriptionRecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic; Short=RA; Short=RuBisCO activase; Flags: Precursor;
Gene nameName=RCA
OrderedLocusNames=At2g39730
ORFNames=T5I7.3
Encoded onName=RCA; OrderedLocusNames=At2g39730; ORFNames=T5I7.3
KeywordsAlternative splicing; ATP-binding; Chloroplast; Complete proteome; Nucleotide-binding; Phosphoprotein; Plastid; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX14212; CAA32429.1; -; mRNA
EMBLM86720; AAA20202.1; -; Genomic_DNA
EMBLM86720; AAA20203.1; -; Genomic_DNA
EMBLAC003000; AAB87122.1; -; Genomic_DNA
EMBLCP002685; AEC09714.1; -; Genomic_DNA
EMBLCP002685; AEC09715.1; -; Genomic_DNA
EMBLAY052703; AAK96607.1; -; mRNA
EMBLAF325049; AAG40401.1; -; mRNA
EMBLAY056108; AAL06995.1; -; mRNA
EMBLBT000710; AAN31853.1; -; mRNA
EMBLAY088487; AAM66023.1; -; mRNA
IPIIPI00518163; -; .
IPIIPI00526733; -; .
PIRS04048; S04048; .
PIRT01002; T01002; .
PIRT01003; T01003; .
RefSeqNP_565913.1; NM_129531.2; .
RefSeqNP_850320.1; NM_179989.2; .
UniGeneAt.25319; -; .
UniGeneAt.47493; -; .
UniGeneAt.75083; -; .
ProteinModelPortalP10896; -; .
SMRP10896; 148-323; .
IntActP10896; 1; .
STRINGP10896; -; .
SWISS-2DPAGEP10896; -; .
World-2DPAGE0003:P10896; -; .
PRIDEP10896; -; .
ProMEXP10896; -; .
EnsemblPlantsAT2G39730.1; AT2G39730.1; AT2G39730; .
GeneID818558; -; .
GenomeReviewsCT485783_GR; AT2G39730; .
KEGGath:AT2G39730; -; .
NMPDRfig|3702.1.peg.11091; -; .
TAIRAt2g39730; -; .
eggNOGKOG0651; -; .
HOGENOMHBG320501; -; .
InParanoidP10896; -; .
OMAWAPTRDD; -; .
ProtClustDBPLN00020; -; .
ArrayExpressP10896; -; .
GenevestigatorP10896; -; .
GOGO:0048046; C:apoplast; IDA:TAIR; .
GOGO:0005618; C:cell wall; IDA:TAIR; .
GOGO:0009941; C:chloroplast envelope; IDA:TAIR; .
GOGO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR; .
GOGO:0005634; C:nucleus; IDA:TAIR; .
GOGO:0010287; C:plastoglobule; IDA:TAIR; .
GOGO:0010319; C:stromule; IDA:TAIR; .
GOGO:0043531; F:ADP binding; IDA:TAIR; .
GOGO:0005524; F:ATP binding; IDA:TAIR; .
GOGO:0046863; F:ribulose-1; 5-bisphosphate carboxylase/oxygenase activator activity; IDA:TAIR
GOGO:0042742; P:defense response to bacterium; IEP:TAIR; .
GOGO:0010150; P:leaf senescence; IMP:TAIR; .
GOGO:0009409; P:response to cold; IEP:TAIR; .
GOGO:0009753; P:response to jasmonic acid stimulus; IEP:TAIR; .
GOGO:0009416; P:response to light stimulus; IEP:TAIR; .
InterProIPR003959; ATPase_AAA_core; .
PfamPF00004; AAA; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server