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Searching in 'World-2DPAGE Repository [0003]' for entry matching: O65719




World-2DPAGE Repository (0003):  O65719


O65719


General information about the entry
View entry in simple text format
Entry nameHSP73_ARATH
Primary accession numberO65719
Secondary accession number(s) Q42345
integrated into World-2DPAGE Repository (0003) on October 14, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on November 15, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Heat shock cognate 70 kDa protein 3; Short=Hsc70.3;.
Gene nameName=HSC70-3
Synonyms=HSP70-3
OrderedLocusNames=At3g09440
ORFNames=F3L24.33, F11F8
Annotated speciesArabidopsis thaliana (Mouse-ear cress) [TaxID: 3702]
TaxonomyEukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core; eudicotyledons; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
References
[1]   MAPPING ON GEL
PubMed=18656559; DOI=10.1016/j.jprot.2008.06.012; [NCBI, EBI, Israel, Japan]
Maldonado A.M., Echevarria-Zomeno S., Jean-Baptiste S., Hernandez M., Jorrin-Novo J.V.
''Evaluation of three different protocols of protein extraction for Arabidopsis thaliana leaf proteome analysis by two-dimensional electrophoresis''
J. Proteomics 71(4):461-472 (2008)
2D PAGE maps for identified proteins
How to interpret a protein

PHENOL_2 {Phenol 2}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
PHENOL_2
  map experimental info
 
PHENOL_2

MAP LOCATIONS:
pI=5.53; Mw=78300  [identification data]
pI=5.49; Mw=78600  [identification data]

IDENTIFICATION: SPOT 20: SeqCov=29%. Mascot (Protein score/CI%=229/100, Total ion score/CI%=108/100) [1]
SPOT 21: SeqCov=16%. Mascot (Protein score/CI%=68/99.78) [1].
MAPPING (identification):
SPOT 20: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 21: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].



TCA-ACETONE_3 {TCA-Acetone 3}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
TCA-ACETONE_3
  map experimental info
 
TCA-ACETONE_3

MAP LOCATIONS:
pI=5.53; Mw=78300  [identification data]
pI=5.49; Mw=78600  [identification data]

IDENTIFICATION: SPOT 20: SeqCov=29%. Mascot (Protein score/CI%=229/100, Total ion score/CI%=108/100) [1]
SPOT 21: SeqCov=16%. Mascot (Protein score/CI%=68/99.78) [1].
MAPPING (identification):
SPOT 20: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 21: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].



TCA-ACETONE-PHENOL_3 {TCA-Acetone-Phenol 3}
Arabidopsis thaliana (Mouse-ear cress)
Tissue: Leaf
TCA-ACETONE-PHENOL_3
  map experimental info
 
TCA-ACETONE-PHENOL_3

MAP LOCATIONS:
pI=5.53; Mw=78300  [identification data]
pI=5.49; Mw=78600  [identification data]

IDENTIFICATION: SPOT 20: SeqCov=29%. Mascot (Protein score/CI%=229/100, Total ion score/CI%=108/100) [1]
SPOT 21: SeqCov=16%. Mascot (Protein score/CI%=68/99.78) [1].
MAPPING (identification):
SPOT 20: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 21: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jesus V. Jorrin-Novo, University of Cordoba, Spain
Cross-references
SWISS-2DPAGEO65719; HSP73_ARATH.
UniProtKB/Swiss-ProtO65719; HSP73_ARATH.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameHSP73_ARATH
Primary accession numberO65719
Secondary accession number(s) Q42345
Sequence was last modified on August 1, 1998 (version 1)
Annotations were last modified on October 19, 2011 (version 93)
Name and origin of the protein
DescriptionRecName: Full=Heat shock cognate 70 kDa protein 3; Short=Hsc70.3;
Gene nameName=HSC70-3
Synonyms=HSP70-3
OrderedLocusNames=At3g09440
ORFNames=F3L24.33, F11F8
Encoded onName=HSC70-3; Synonyms=HSP70-3; OrderedLocusNames=At3g09440; ORFNames=F3L24.33, F11F8
KeywordsATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLY17053; CAA76606.1; -; mRNA
EMBLAC011436; AAF14038.1; -; Genomic_DNA
EMBLAC016661; AAF23276.1; -; Genomic_DNA
EMBLCP002686; AEE74767.1; -; Genomic_DNA
EMBLCP002686; AEE74768.1; -; Genomic_DNA
EMBLAY050896; AAK92833.1; -; mRNA
EMBLAY096676; AAM20310.1; -; mRNA
EMBLAY102116; AAM26685.1; -; mRNA
EMBLBT001066; AAN46823.1; -; mRNA
EMBLF20026; CAA23383.1; -; mRNA
IPIIPI00543340; -; .
RefSeqNP_001189847.1; NM_001202918.1; .
RefSeqNP_187555.1; NM_111778.3; .
UniGeneAt.22293; -; .
ProteinModelPortalO65719; -; .
SMRO65719; 1-622; .
IntActO65719; 1; .
STRINGO65719; -; .
SWISS-2DPAGEO65719; -; .
World-2DPAGE0003:O65719; -; .
PRIDEO65719; -; .
EnsemblPlantsAT3G09440.1; AT3G09440.1; AT3G09440; .
EnsemblPlantsAT3G09440.2; AT3G09440.2; AT3G09440; .
GeneID820102; -; .
GenomeReviewsBA000014_GR; AT3G09440; .
KEGGath:AT3G09440; -; .
NMPDRfig|3702.1.peg.12959; -; .
TAIRAt3g09440; -; .
eggNOGKOG0101; -; .
HOGENOMHBG334976; -; .
InParanoidO65719; -; .
OMALAECDEF; -; .
PhylomeDBO65719; -; .
ProtClustDBCLSN2915682; -; .
ArrayExpressO65719; -; .
GenevestigatorO65719; -; .
GermOnlineAT3G09440; Arabidopsis thaliana; .
GOGO:0048046; C:apoplast; IDA:TAIR; .
GOGO:0005618; C:cell wall; IDA:TAIR; .
GOGO:0009507; C:chloroplast; IDA:TAIR; .
GOGO:0022626; C:cytosolic ribosome; IDA:TAIR; .
GOGO:0005886; C:plasma membrane; IDA:TAIR; .
GOGO:0009506; C:plasmodesma; IDA:TAIR; .
GOGO:0005774; C:vacuolar membrane; IDA:TAIR; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0002020; F:protease binding; IPI:UniProtKB; .
GOGO:0046686; P:response to cadmium ion; IEP:TAIR; .
GOGO:0009408; P:response to heat; IEP:TAIR; .
GOGO:0080167; P:response to karrikin; IEP:TAIR; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server