World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

map experimental info

HIPP_SYNAP

MAP LOCATIONS:

SPOT 548:

pI=5.31; Mw=42107

[identification data]

SPOT 552:

pI=5.31; Mw=42107

[identification data]

SPOT 568:

pI=5.31; Mw=42107

[identification data]

SPOT 570:

pI=5.31; Mw=42107

[identification data]

SPOT 711:

pI=5.31; Mw=42107

[identification data]

SPOT 718:

pI=5.31; Mw=42107

[identification data]

SPOT 779:

pI=5.31; Mw=42107

[identification data]

SPOT 801:

pI=5.31; Mw=42107

[identification data]

SPOT 804:

pI=5.31; Mw=42107

[identification data]

SPOT 818:

pI=5.31; Mw=42107

[identification data]

SPOT 821:

pI=5.31; Mw=42107

[identification data]

SPOT 845:

pI=5.31; Mw=42107

[identification data]

SPOT 846:

pI=5.31; Mw=42107

[identification data]

SPOT 1003:

pI=5.31; Mw=42107

[identification data]

SPOT 1066:

pI=5.31; Mw=42107

[identification data]

SPOT 1076:

pI=5.31; Mw=42107

[identification data]

SPOT 1078:

pI=5.31; Mw=42107

[identification data]

SPOT 1116:

pI=5.31; Mw=42107

[identification data]

IDENTIFICATION: SPOT 548: Peptides number=8. SeqCov=33.3%. MOWSE score=4.88E+13 [1]
SPOT 552: Peptides number=5. SeqCov=21.1%. MOWSE score=1.77E+7 [1]; SPOT 568: Peptides number=6. SeqCov=23.5%. MOWSE score=1.47E+7 [1]; SPOT 570: Peptides number=8. SeqCov=32.8%. MOWSE score=1.46E+6 [1]; SPOT 711: Peptides number=7. SeqCov=33.9%. MOWSE score=4.10E+11 [1]; SPOT 718: Peptides number=5. SeqCov=24%. MOWSE score=4.75E+6 [1]; SPOT 779: Peptides number=5. SeqCov=24%. MOWSE score=4.75E+6 [1]; SPOT 801: Peptides number=8. SeqCov=27.7%. MOWSE score=8.47E+6 [1]; SPOT 804: Peptides number=3. SeqCov=13.6%. MOWSE score=8.80E+4 [1]; SPOT 818: Peptides number=3. SeqCov=15.7%. MOWSE score=4.10E+3 [1]; SPOT 821: Peptides number=3. SeqCov=13.6%. MOWSE score=5.78E+4 [1]; SPOT 845: Peptides number=3. SeqCov=12.5%. MOWSE score=3.79E+3 [1]; SPOT 846: Peptides number=4. SeqCov=18.4%. MOWSE score=1.18E+4 [1]; SPOT 1003: Peptides number=6. SeqCov=22.9%. MOWSE score=6.61E+5 [1]; SPOT 1066: Peptides number=7. SeqCov=38.4%. MOWSE score=1.06E+10 [1]; SPOT 1076: Peptides number=6. SeqCov=32%. MOWSE score=1.06E+10 [1]; SPOT 1078: Peptides number=4. SeqCov=17.1%. MOWSE score=1.23E+4 [1]; SPOT 1116: Peptides number=7. SeqCov=40%. MOWSE score=6.41E+9 [1].
MAPPING (identification):

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

P63261; ACTG_HUMAN.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ACTG_HUMAN
Primary accession number	P63261
Secondary accession number(s)	A8K7C2 P02571 P14104 P99022 Q5U032 Q96E67
Sequence was last modified on	July 21, 1986 (version 1)
Annotations were last modified on	December 14, 2011 (version 96)
Name and origin of the protein
Description	RecName: Full=Actin, cytoplasmic 2; AltName: Full=Gamma-actin; Contains: RecName: Full=Actin, cytoplasmic 2, N-terminally processed;
Gene name	Name=ACTG1 Synonyms=ACTB, ACTG
Encoded on	Name=ACTG1; Synonyms=ACTB, ACTG
Keywords	Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Deafness; Direct protein sequencing; Disease mutation; Methylation; Non-syndromic deafness; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X04098; CAA27723.1; -; mRNA
EMBL	M19283; AAA51579.1; -; Genomic_DNA
EMBL	AK291937; BAF84626.1; -; mRNA
EMBL	BT019856; AAV38659.1; -; mRNA
EMBL	BC000292; AAH00292.1; -; mRNA
EMBL	BC001920; AAH01920.1; -; mRNA
EMBL	BC007442; AAH07442.1; -; mRNA
EMBL	BC009848; AAH09848.1; -; mRNA
EMBL	BC010999; AAH10999.1; -; mRNA
EMBL	BC012050; AAH12050.1; -; mRNA
EMBL	BC015005; AAH15005.1; -; mRNA
EMBL	BC015695; AAH15695.1; -; mRNA
EMBL	BC015779; AAH15779.1; -; mRNA
EMBL	BC018774; AAH18774.1; -; mRNA
EMBL	BC053572; AAH53572.1; -; mRNA
EMBL	M16247; AAA51580.1; -; mRNA
IPI	IPI00021440; -; .
PIR	A28098; ATHUG; .
PIR	JC5818; JC5818; .
RefSeq	NP_001186883.1; NM_001199954.1; .
RefSeq	NP_001605.1; NM_001614.3; .
UniGene	Hs.514581; -; .
ProteinModelPortal	P63261; -; .
SMR	P63261; 2-375; .
IntAct	P63261; 34; .
MINT	MINT-4998686; -; .
STRING	P63261; -; .
PhosphoSite	P63261; -; .
DMDM	54036678; -; .
SWISS-2DPAGE	P63261; -; .
DOSAC-COBS-2DPAGE	P60709_OR_P63261; -; .
DOSAC-COBS-2DPAGE	P63261; -; .
OGP	P63261; -; .
PMMA-2DPAGE	P63261; -; .
REPRODUCTION-2DPAGE	P63261; -; .
PRIDE	P63261; -; .
Ensembl	ENST00000331925; ENSP00000331514; ENSG00000184009; .
GeneID	71; -; .
KEGG	hsa:71; -; .
UCSC	uc002kaj.1; human; .
CTD	71; -; .
GeneCards	GC17M074925; -; .
H-InvDB	HIX0020804; -; .
HGNC	HGNC:144; ACTG1; .
HPA	CAB013531; -; .
MIM	102560; gene; .
MIM	604717; phenotype; .
neXtProt	NX_P63261; -; .
Orphanet	90635; Autosomal dominant nonsyndromic sensorineural deafness type DFNA; .
PharmGKB	PA24468; -; .
HOVERGEN	HBG003771; -; .
InParanoid	P63261; -; .
OMA	IAMEEEI; -; .
OrthoDB	EOG41JZC9; -; .
PhylomeDB	P63261; -; .
Reactome	REACT_111045; Developmental Biology; .
Reactome	REACT_111155; Cell-Cell communication; .
Reactome	REACT_11123; Membrane Trafficking; .
Reactome	REACT_604; Hemostasis; .
NextBio	279; -; .
PMAP-CutDB	P63261; -; .
ArrayExpress	P63261; -; .
Bgee	P63261; -; .
CleanEx	HS_ACTB; -; .
CleanEx	HS_ACTG1; -; .
Genevestigator	P63261; -; .
GermOnline	ENSG00000184009; Homo sapiens; .
GO	GO:0005829; C:cytosol; TAS:Reactome; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0042802; F:identical protein binding; IPI:IntAct; .
GO	GO:0005200; F:structural constituent of cytoskeleton; IC:UniProtKB; .
GO	GO:0034332; P:adherens junction organization; TAS:Reactome; .
GO	GO:0007411; P:axon guidance; TAS:Reactome; .
GO	GO:0007596; P:blood coagulation; TAS:Reactome; .
GO	GO:0034329; P:cell junction assembly; TAS:Reactome; .
GO	GO:0006928; P:cellular component movement; TAS:UniProtKB; .
InterPro	IPR004000; Actin-like; .
InterPro	IPR020902; Actin/actin-like_CS; .
InterPro	IPR004001; Actin_CS; .
KO	K05692; -; .
PANTHER	PTHR11937; Actin_like; 1; .
Pfam	PF00022; Actin; 1; .
PRINTS	PR00190; ACTIN; .
SMART	SM00268; ACTIN; 1; .
PROSITE	PS00406; ACTINS_1; 1; .
PROSITE	PS00432; ACTINS_2; 1; .
PROSITE	PS01132; ACTINS_ACT_LIKE; 1; .

Searching in 'World-2DPAGE Repository [0026]' for entry matching: P63261

World-2DPAGE Repository (0026): P63261

P63261

General information about the entry

View entry in simple text format

Entry name

ACTG_HUMAN

Primary accession number

P63261

integrated into World-2DPAGE Repository (0026) on

September 23, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Actin, cytoplasmic 2; AltName: Full=Gamma-actin; Contains: RecName: Full=Actin, cytoplasmic 2, N-terminally processed;.

Gene name

Name=ACTG1
Synonyms=ACTB, ACTG

Annotated species

Homo sapiens (Human) [TaxID: 9606]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

References

[1]

2D GEL CHARACTERIZATION
PubMed=19862763; DOI=10.1002/pmic.200800836; [NCBI, EBI, Israel, Japan]
Moerbt N., Moegel I., Kalkhof S., Feltens R., Roeder-Stolinski C., Zheng J., Vogt C., Lehmann I., Von Bergen M.
''Proteome changes in human bronchoalveolar cells following styrene exposure indicate invement of oxidative stress in the molecular-response mechanism''
Proteomics 9(21):4920-4933 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

A549_HUMAN {Proteome map of A549 cells}
Homo sapiens (Human)
Tissue: Lung

map experimental info

A549_HUMAN

MAP LOCATIONS:

SPOT 177:

pI=5.31; Mw=41793

[identification data]

IDENTIFICATION: SPOT 177: Peptides=7. SeqCov=19%. Mascot Score=292 [1].
MAPPING (identification):

SPOT 177: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Nora Moerbt, UFZ-Helmholtz-Centre for Environmental Research, Germany

Cross-references

DOSAC-COBS 2D-PAGE

REPRODUCTION-2DPAGE

SWISS-2DPAGE

UniProtKB/Swiss-Prot

World-2DPAGE Repository

P63261; ACTG_HUMAN.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	ACTG_HUMAN
Primary accession number	P63261
Secondary accession number(s)	A8K7C2 P02571 P14104 P99022 Q5U032 Q96E67
Sequence was last modified on	July 21, 1986 (version 1)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Actin, cytoplasmic 2; AltName: Full=Gamma-actin; Contains: RecName: Full=Actin, cytoplasmic 2, N-terminally processed;
Gene name	Name=ACTG1 Synonyms=ACTB, ACTG
Encoded on	Name=ACTG1; Synonyms=ACTB, ACTG
Keywords	Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Deafness; Direct protein sequencing; Disease mutation; Methylation; Non-syndromic deafness; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X04098; CAA27723.1; -; mRNA
EMBL	M19283; AAA51579.1; -; Genomic_DNA
EMBL	AK291937; BAF84626.1; -; mRNA
EMBL	BT019856; AAV38659.1; -; mRNA
EMBL	BC000292; AAH00292.1; -; mRNA
EMBL	BC001920; AAH01920.1; -; mRNA
EMBL	BC007442; AAH07442.1; -; mRNA
EMBL	BC009848; AAH09848.1; -; mRNA
EMBL	BC010999; AAH10999.1; -; mRNA
EMBL	BC012050; AAH12050.1; -; mRNA
EMBL	BC015005; AAH15005.1; -; mRNA
EMBL	BC015695; AAH15695.1; -; mRNA
EMBL	BC015779; AAH15779.1; -; mRNA
EMBL	BC018774; AAH18774.1; -; mRNA
EMBL	BC053572; AAH53572.1; -; mRNA
EMBL	M16247; AAA51580.1; -; mRNA
IPI	IPI00021440; -; .
PIR	A28098; ATHUG; .
PIR	JC5818; JC5818; .
RefSeq	NP_001186883.1; NM_001199954.1; .
RefSeq	NP_001605.1; NM_001614.3; .
UniGene	Hs.514581; -; .
ProteinModelPortal	P63261; -; .
SMR	P63261; 2-375; .
IntAct	P63261; 32; .
MINT	MINT-4998686; -; .
STRING	P63261; -; .
PhosphoSite	P63261; -; .
SWISS-2DPAGE	P63261; -; .
DOSAC-COBS-2DPAGE	P60709_OR_P63261; -; .
DOSAC-COBS-2DPAGE	P63261; -; .
OGP	P63261; -; .
PMMA-2DPAGE	P63261; -; .
REPRODUCTION-2DPAGE	P63261; -; .
PRIDE	P63261; -; .
Ensembl	ENST00000331925; ENSP00000331514; ENSG00000184009; .
GeneID	71; -; .
KEGG	hsa:71; -; .
UCSC	uc002kaj.1; human; .
CTD	71; -; .
GeneCards	GC17M074925; -; .
H-InvDB	HIX0020804; -; .
HGNC	HGNC:144; ACTG1; .
HPA	CAB013531; -; .
MIM	102560; gene; .
MIM	604717; phenotype; .
neXtProt	NX_P63261; -; .
Orphanet	90635; Autosomal dominant nonsyndromic sensorineural deafness type DFNA; .
PharmGKB	PA24468; -; .
HOVERGEN	HBG003771; -; .
InParanoid	P63261; -; .
OMA	IAMEEEI; -; .
OrthoDB	EOG41JZC9; -; .
PhylomeDB	P63261; -; .
Reactome	REACT_111045; Developmental Biology; .
Reactome	REACT_111155; Cell-Cell communication; .
Reactome	REACT_11123; Membrane Trafficking; .
Reactome	REACT_604; Hemostasis; .
NextBio	279; -; .
PMAP-CutDB	P63261; -; .
ArrayExpress	P63261; -; .
Bgee	P63261; -; .
CleanEx	HS_ACTB; -; .
CleanEx	HS_ACTG1; -; .
Genevestigator	P63261; -; .
GermOnline	ENSG00000184009; Homo sapiens; .
GO	GO:0005829; C:cytosol; TAS:Reactome; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0042802; F:identical protein binding; IPI:IntAct; .
GO	GO:0005200; F:structural constituent of cytoskeleton; IC:UniProtKB; .
GO	GO:0034332; P:adherens junction organization; TAS:Reactome; .
GO	GO:0007411; P:axon guidance; TAS:Reactome; .
GO	GO:0007596; P:blood coagulation; TAS:Reactome; .
GO	GO:0034329; P:cell junction assembly; TAS:Reactome; .
GO	GO:0006928; P:cellular component movement; TAS:UniProtKB; .
InterPro	IPR004000; Actin-like; .
InterPro	IPR020902; Actin/actin-like_CS; .
InterPro	IPR004001; Actin_CS; .
KO	K05692; -; .
PANTHER	PTHR11937; Actin_like; 1; .
Pfam	PF00022; Actin; 1; .
PRINTS	PR00190; ACTIN; .
SMART	SM00268; ACTIN; 1; .
PROSITE	PS00406; ACTINS_1; 1; .
PROSITE	PS00432; ACTINS_2; 1; .
PROSITE	PS01132; ACTINS_ACT_LIKE; 1; .

Searching in 'World-2DPAGE Repository [0001]' for entry matching: P63261

World-2DPAGE Repository (0001): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0001)

Searching in 'World-2DPAGE Repository [0002]' for entry matching: P63261

World-2DPAGE Repository (0002): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0002)

Searching in 'World-2DPAGE Repository [0003]' for entry matching: P63261

World-2DPAGE Repository (0003): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0003)

Searching in 'World-2DPAGE Repository [0004]' for entry matching: P63261

World-2DPAGE Repository (0004): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0004)

Searching in 'World-2DPAGE Repository [0005]' for entry matching: P63261

World-2DPAGE Repository (0005): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0005)

Searching in 'World-2DPAGE Repository [0006]' for entry matching: P63261

World-2DPAGE Repository (0006): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0006)

Searching in 'World-2DPAGE Repository [0007]' for entry matching: P63261

World-2DPAGE Repository (0007): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0007)

Searching in 'World-2DPAGE Repository [0008]' for entry matching: P63261

World-2DPAGE Repository (0008): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0008)

Searching in 'World-2DPAGE Repository [0009]' for entry matching: P63261

World-2DPAGE Repository (0009): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0009)

Searching in 'World-2DPAGE Repository [0010]' for entry matching: P63261

World-2DPAGE Repository (0010): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0010)

Searching in 'World-2DPAGE Repository [0011]' for entry matching: P63261

World-2DPAGE Repository (0011): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0011)

Searching in 'World-2DPAGE Repository [0012]' for entry matching: P63261

World-2DPAGE Repository (0012): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0012)

Searching in 'World-2DPAGE Repository [0014]' for entry matching: P63261

World-2DPAGE Repository (0014): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0014)

Searching in 'World-2DPAGE Repository [0017]' for entry matching: P63261

World-2DPAGE Repository (0017): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0017)

Searching in 'World-2DPAGE Repository [0018]' for entry matching: P63261

World-2DPAGE Repository (0018): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0018)

Searching in 'World-2DPAGE Repository [0020]' for entry matching: P63261

World-2DPAGE Repository (0020): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0020)

Searching in 'World-2DPAGE Repository [0021]' for entry matching: P63261

World-2DPAGE Repository (0021): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0021)

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P63261

World-2DPAGE Repository (0022): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0022)

Searching in 'World-2DPAGE Repository [0023]' for entry matching: P63261

World-2DPAGE Repository (0023): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0023)

Searching in 'World-2DPAGE Repository [0024]' for entry matching: P63261

World-2DPAGE Repository (0024): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0024)

Searching in 'World-2DPAGE Repository [0027]' for entry matching: P63261

World-2DPAGE Repository (0027): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0027)

Searching in 'World-2DPAGE Repository [0029]' for entry matching: P63261

World-2DPAGE Repository (0029): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0029)

Searching in 'World-2DPAGE Repository [0030]' for entry matching: P63261

World-2DPAGE Repository (0030): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0030)

Searching in 'World-2DPAGE Repository [0031]' for entry matching: P63261

World-2DPAGE Repository (0031): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0031)

Searching in 'World-2DPAGE Repository [0032]' for entry matching: P63261

World-2DPAGE Repository (0032): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0032)

Searching in 'World-2DPAGE Repository [0034]' for entry matching: P63261

World-2DPAGE Repository (0034): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0034)

Searching in 'World-2DPAGE Repository [0035]' for entry matching: P63261

World-2DPAGE Repository (0035): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0035)

Searching in 'World-2DPAGE Repository [0038]' for entry matching: P63261

World-2DPAGE Repository (0038): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0038)

Searching in 'World-2DPAGE Repository [0043]' for entry matching: P63261

World-2DPAGE Repository (0043): P63261

This protein does not exist in the current release of World-2DPAGE Repository (0043)

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server

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