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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00330




SWISS-2DPAGE:  P00330


P00330


General information about the entry
View entry in simple text format
Entry nameADH1_YEAST
Primary accession numberP00330
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Alcohol dehydrogenase 1; EC=1.1.1.1; AltName: Full=Alcohol dehydrogenase I; AltName: Full=YADH-1;.
Gene nameName=ADH1
Synonyms=ADC1
OrderedLocusNames=YOL086C
ORFNames=O0947
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=82271855; PubMed=7050667; [NCBI, Expasy, EBI, Israel, Japan]
Ludwig J.R., Foy J.J., Elliott S.G., McLaughlin C.S.
''''''Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae'';'';''
Mol. Cell. Biol. 2(1):117-126(1982)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=6.60; Mw=41097
pI=6.45; Mw=41184
pI=6.69; Mw=40924

MAPPING (identification):
AMINO ACID COMPOSITION ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY LUDWIG ET AL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP00330; ADH1_YEAST.
UniProtKB/Swiss-ProtP00330; ADH1_YEAST.
YEPD4398.
YEPD5340.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameADH1_YEAST
Primary accession numberP00330
Secondary accession number(s) D6W1Y3
Sequence was last modified on October 5, 2010 (version 5)
Annotations were last modified on October 19, 2011 (version 134)
Name and origin of the protein
DescriptionRecName: Full=Alcohol dehydrogenase 1; EC=1.1.1.1; AltName: Full=Alcohol dehydrogenase I; AltName: Full=YADH-1;
Gene nameName=ADH1
Synonyms=ADC1
OrderedLocusNames=YOL086C
ORFNames=O0947
Encoded onName=ADH1; Synonyms=ADC1; OrderedLocusNames=YOL086C; ORFNames=O0947
Keywords3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV01292; CAA24601.1; -; Genomic_DNA
EMBLM38456; AAA34410.1; -; Genomic_DNA
EMBLX83121; CAA58193.1; -; Genomic_DNA
EMBLZ74828; CAA99098.1; -; Genomic_DNA
EMBLV01291; CAA24600.1; -; Genomic_DNA
EMBLBK006948; DAA10699.1; -; Genomic_DNA
PIRS57383; DEBYA; .
RefSeqNP_014555.1; NM_001183340.1; .
PDB2HCY; X-ray; 2.44 A; A/B/C/D=2-347
PDBsum2HCY; -; .
ProteinModelPortalP00330; -; .
SMRP00330; 2-348; .
DIPDIP-1143N; -; .
IntActP00330; 198; .
MINTMINT-642403; -; .
STRINGP00330; -; .
SWISS-2DPAGEP00330; -; .
COMPLUYEAST-2DPAGEP00330; -; .
PeptideAtlasP00330; -; .
PRIDEP00330; -; .
EnsemblFungiYOL086C; YOL086C; YOL086C; .
GeneID854068; -; .
KEGGsce:YOL086C; -; .
SGDS000005446; ADH1; .
eggNOGfuNOG04605; -; .
GeneTreeEFGT00050000001793; -; .
HOGENOMHBG753318; -; .
OMAKSNESNC; -; .
OrthoDBEOG44QX8R; -; .
PhylomeDBP00330; -; .
BioCycMetaCyc:MONOMER-11724; -; .
ArrayExpressP00330; -; .
GenevestigatorP00330; -; .
GermOnlineYOL086C; Saccharomyces cerevisiae; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0001950; C:plasma membrane enriched fraction; IDA:SGD; .
GOGO:0004022; F:alcohol dehydrogenase (NAD) activity; IDA:SGD; .
GOGO:0019170; F:methylglyoxal reductase (NADH-dependent) activity; IDA:SGD; .
GOGO:0008270; F:zinc ion binding; IEA:InterPro; .
GOGO:0043458; P:ethanol biosynthetic process involved in glucose fermentation to ethanol; IMP:SGD; .
GOGO:0006116; P:NADH oxidation; IDA:SGD; .
InterProIPR013149; ADH_C; .
InterProIPR013154; ADH_GroES-like; .
InterProIPR002085; ADH_SF_Zn-type; .
InterProIPR002328; ADH_Zn_CS; .
InterProIPR011032; GroES-like; .
PANTHERPTHR11695; ADH_Sf_Zn; 1; .
PfamPF08240; ADH_N; 1; .
PfamPF00107; ADH_zinc_N; 1; .
SUPFAMSSF50129; GroES_like; 1; .
PROSITEPS00059; ADH_ZINC; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server