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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00924




SWISS-2DPAGE:  P00924


P00924


General information about the entry
View entry in simple text format
Entry nameENO1_YEAST
Primary accession numberP00924
integrated into SWISS-2DPAGE on February 1, 1995 (release 1)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Enolase 1; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase 1; AltName: Full=2-phosphoglycerate dehydratase 1;.
Gene nameName=ENO1
Synonyms=ENOA, HSP48
OrderedLocusNames=YGR254W
ORFNames=G9160
Annotated speciesSaccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]
TaxonomyEukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
References
[1]   MAPPING ON GEL
MEDLINE=96314060; PubMed=8740180; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F.
''''''The yeast SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):556-565(1996)
[2]   MAPPING ON GEL
MEDLINE=82271855; PubMed=7050667; [NCBI, Expasy, EBI, Israel, Japan]
Ludwig J.R., Foy J.J., Elliott S.G., McLaughlin C.S.
''''''Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae'';'';''
Mol. Cell. Biol. 2(1):117-126(1982)
[3]   MAPPING ON GEL
MEDLINE=89073918; PubMed=3332961; [NCBI, Expasy, EBI, Israel, Japan]
Bataille N., Peypouquet M.-F., Boucherie H.
''''''Identification of polypeptides of the carbon metabolism machinery on the two-dimensional protein map of Saccharomyces cerevisiae. Location of 23 additional polypeptides'';'';''
Yeast 3(1):11-21(1987)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

YEAST {Saccharomyces cerevisiae}
Saccharomyces cerevisiae (Baker's yeast)
YEAST
  map experimental info
  protein estimated location
 
YEAST

MAP LOCATIONS:
pI=6.50; Mw=46545
pI=6.61; Mw=46284

MAPPING (identification):
AMINO ACID COMPOSITION ANALYSIS, MICROSEQUENCING [1] AND IDENTIFIED ON 2-D GELS BY LUDWIG ET AL [2] AND BATAILLE ET AL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP00924; ENO1_YEAST.
UCD-2DPAGEP00924; ENO1_YEAST.
UniProtKB/Swiss-ProtP00924; ENO1_YEAST.
YEPD4488.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameENO1_YEAST
Primary accession numberP00924
Secondary accession number(s) D6VV34 P99013
Sequence was last modified on October 5, 2010 (version 3)
Annotations were last modified on October 19, 2011 (version 141)
Name and origin of the protein
DescriptionRecName: Full=Enolase 1; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase 1; AltName: Full=2-phosphoglycerate dehydratase 1;
Gene nameName=ENO1
Synonyms=ENOA, HSP48
OrderedLocusNames=YGR254W
ORFNames=G9160
Encoded onName=ENO1; Synonyms=ENOA, HSP48; OrderedLocusNames=YGR254W; ORFNames=G9160
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01322; AAA88712.1; -; Genomic_DNA
EMBLX99228; CAA67616.1; -; Genomic_DNA
EMBLZ73039; CAA97283.1; -; Genomic_DNA
EMBLBK006941; DAA08345.1; -; Genomic_DNA
PIRS64586; NOBY; .
RefSeqNP_011770.1; NM_001181383.1; .
PDB1EBG; X-ray; 2.10 A; A/B=2-436
PDB1EBH; X-ray; 1.90 A; A/B=2-436
PDB1ELS; X-ray; 2.40 A; A=2-436
PDB1L8P; X-ray; 2.10 A; A/B/C/D=2-437
PDB1NEL; X-ray; 2.60 A; A=2-436
PDB1ONE; X-ray; 1.80 A; A/B=2-437
PDB1P43; X-ray; 1.80 A; A/B=2-437
PDB1P48; X-ray; 2.00 A; A/B=2-437
PDB2AL1; X-ray; 1.50 A; A/B=2-436
PDB2AL2; X-ray; 1.85 A; A/B=2-436
PDB2ONE; X-ray; 2.00 A; A/B=2-437
PDB2XGZ; X-ray; 1.80 A; A/B=2-437
PDB2XH0; X-ray; 1.70 A; A/B/C/D=2-437
PDB2XH2; X-ray; 1.80 A; A/B/C/D=2-437
PDB2XH4; X-ray; 1.70 A; A/B/C/D=2-437
PDB2XH7; X-ray; 1.80 A; A/B=2-437
PDB3ENL; X-ray; 2.25 A; A=2-437
PDB4ENL; X-ray; 1.90 A; A=2-436
PDB5ENL; X-ray; 2.20 A; A=2-436
PDB6ENL; X-ray; 2.20 A; A=2-436
PDB7ENL; X-ray; 2.20 A; A=2-436
PDBsum1EBG; -; .
PDBsum1EBH; -; .
PDBsum1ELS; -; .
PDBsum1L8P; -; .
PDBsum1NEL; -; .
PDBsum1ONE; -; .
PDBsum1P43; -; .
PDBsum1P48; -; .
PDBsum2AL1; -; .
PDBsum2AL2; -; .
PDBsum2ONE; -; .
PDBsum2XGZ; -; .
PDBsum2XH0; -; .
PDBsum2XH2; -; .
PDBsum2XH4; -; .
PDBsum2XH7; -; .
PDBsum3ENL; -; .
PDBsum4ENL; -; .
PDBsum5ENL; -; .
PDBsum6ENL; -; .
PDBsum7ENL; -; .
ProteinModelPortalP00924; -; .
SMRP00924; 2-437; .
DIPDIP-5561N; -; .
IntActP00924; 16; .
MINTMINT-574648; -; .
STRINGP00924; -; .
Allergome786; Sac c Enolase; .
SWISS-2DPAGEP00924; -; .
COMPLUYEAST-2DPAGEP00924; -; .
UCD-2DPAGEP00924; -; .
PeptideAtlasP00924; -; .
PRIDEP00924; -; .
EnsemblFungiYGR254W; YGR254W; YGR254W; .
GeneID853169; -; .
KEGGsce:YGR254W; -; .
SGDS000003486; ENO1; .
eggNOGfuNOG04478; -; .
GeneTreeEFGT00050000005340; -; .
HOGENOMHBG726599; -; .
OMAKVEIGMD; -; .
OrthoDBEOG48WG9D; -; .
PhylomeDBP00924; -; .
BRENDA4.2.1.11; 984; .
ArrayExpressP00924; -; .
GenevestigatorP00924; -; .
GermOnlineYGR254W; Saccharomyces cerevisiae; .
GOGO:0000324; C:fungal-type vacuole; IDA:SGD; .
GOGO:0005739; C:mitochondrion; IDA:SGD; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IDA:SGD; .
GOGO:0001950; C:plasma membrane enriched fraction; IDA:SGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IMP:SGD; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0006094; P:gluconeogenesis; IEP:SGD; .
GOGO:0006096; P:glycolysis; IMP:SGD; .
GOGO:0032889; P:regulation of vacuole fusion; non-autophagic; IMP:SGD
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
PRINTSPR00148; ENOLASE; .
TIGRFAMsTIGR01060; Eno; 1; .
PROSITEPS00164; ENOLASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server