Attention: World-2DPAGE is no longer maintained.

SWISS-2DPAGE  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Select Remote Interfaces [All Interfaces] World-2DPAGE Portal World-2DPAGE Repository Exclude local DBs has only effect if a remote interface is selected

Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P02931 SWISS-2DPAGE:  P02931 P02931 General information about the entry View entry in simple text format Entry name OMPF_ECOLI Primary accession number P02931 integrated into SWISS-2DPAGE on  August 1, 1995 (release 2) 2D Annotations were last modified on May 15, 2003 (version 2) General Annotations were last modified on  May 19, 2011 (version 11) Name and origin of the protein Description RecName: Full=Outer membrane protein F; AltName: Full=Outer membrane protein 1A; AltName: Full=Outer membrane protein B; AltName: Full=Outer membrane protein IA; AltName: Full=Porin OmpF; Flags: Precursor;. Gene name Name=ompF Synonyms=cmlB, coa, cry, tolF OrderedLocusNames=b0929, JW0912 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996) [2]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996) [3]   MAPPING ON GEL PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002) Comments SUBUNIT: HOMOTRIMER 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000L58: pI=4.60; Mw=36242 MAPPING (identification): GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]. ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WNW: pI=4.56; Mw=37316   [identification data] SPOT 2D-001WNX: pI=4.64; Mw=37000   [identification data] SPOT 2D-001WO6: pI=4.71; Mw=37000   [identification data] EXPRESSION: decrease after benzoic acid treatment [3]. MAPPING (identification): Tandem mass spectrometry [3]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references 2DBase-Ecoli P02931; OMPF_ECOLI. ECO2DBASE B036.0; 6TH EDITION. UniProtKB/Swiss-Prot P02931; OMPF_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name OMPF_ECOLI Primary accession number P02931 Sequence was last modified on  July 21, 1986 (version 1) Annotations were last modified on  October 19, 2011 (version 135) Name and origin of the protein Description RecName: Full=Outer membrane protein F; AltName: Full=Outer membrane protein 1A; AltName: Full=Outer membrane protein B; AltName: Full=Outer membrane protein IA; AltName: Full=Porin OmpF; Flags: Precursor; Gene name Name=ompF Synonyms=cmlB, coa, cry, tolF OrderedLocusNames=b0929, JW0912 Encoded on Name=ompF; Synonyms=cmlB, coa, cry, tolF; OrderedLocusNames=b0929, JW0912 Keywords 3D-structure; Cell membrane; Cell outer membrane; Complete proteome; Direct protein sequencing; Ion transport; Membrane; Porin; Reference proteome; Signal; Transmembrane; Transmembrane beta strand; Transport. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL J01655; AAA24244.1; -; Genomic_DNA EMBL U00096; AAC74015.1; -; Genomic_DNA EMBL AP009048; BAA35675.1; -; Genomic_DNA PIR A93449; MMECF; . RefSeq NP_415449.1; NC_000913.2; . PDB 1BT9; X-ray; 3.00 A; A=23-362 PDB 1GFM; X-ray; 3.50 A; A=23-362 PDB 1GFN; X-ray; 3.10 A; A=23-362 PDB 1GFO; X-ray; 3.30 A; A=23-362 PDB 1GFP; X-ray; 2.70 A; A=23-362 PDB 1GFQ; X-ray; 2.80 A; A=23-362 PDB 1HXT; X-ray; 2.40 A; A=23-362 PDB 1HXU; X-ray; 3.00 A; A=23-362 PDB 1HXX; X-ray; 2.20 A; A=23-362 PDB 1MPF; X-ray; 3.00 A; A=23-362 PDB 1OPF; X-ray; 3.20 A; A/B/C/D/E/F=23-362 PDB 2OMF; X-ray; 2.40 A; A=23-362 PDB 2ZFG; X-ray; 1.59 A; A=23-362 PDB 2ZLD; X-ray; 3.00 A; A/B=23-362 PDB 3FYX; X-ray; 3.40 A; A=23-362 PDB 3HW9; X-ray; 2.61 A; A/B=1-362 PDB 3HWB; X-ray; 3.00 A; A/B=1-362 PDB 3K19; X-ray; 3.79 A; A/B/C/D/E/F/G/H/I/J/K/L=23-362 PDB 3K1B; X-ray; 4.39 A; A/B/C/D=23-362 PDBsum 1BT9; -; . PDBsum 1GFM; -; . PDBsum 1GFN; -; . PDBsum 1GFO; -; . PDBsum 1GFP; -; . PDBsum 1GFQ; -; . PDBsum 1HXT; -; . PDBsum 1HXU; -; . PDBsum 1HXX; -; . PDBsum 1MPF; -; . PDBsum 1OPF; -; . PDBsum 2OMF; -; . PDBsum 2ZFG; -; . PDBsum 2ZLD; -; . PDBsum 3FYX; -; . PDBsum 3HW9; -; . PDBsum 3HWB; -; . PDBsum 3K19; -; . PDBsum 3K1B; -; . ProteinModelPortal P02931; -; . SMR P02931; 23-362; . DIP DIP-10398N; -; . IntAct P02931; 2; . TCDB 1.B.1.1.1; general bacterial porin (GBP) family; . SWISS-2DPAGE P02931; -; . 2DBase-Ecoli P02931; -; . ECO2DBASE B036.0; 6TH EDITION; . PRIDE P02931; -; . EnsemblBacteria EBESCT00000004263; EBESCP00000004263; EBESCG00000003475; . EnsemblBacteria EBESCT00000016555; EBESCP00000015846; EBESCG00000015615; . GeneID 945554; -; . GenomeReviews AP009048_GR; JW0912; . GenomeReviews U00096_GR; b0929; . KEGG ecj:JW0912; -; . KEGG eco:b0929; -; . EchoBASE EB0665; -; . EcoGene EG10671; ompF; . eggNOG COG3203; -; . GeneTree EBGT00050000008697; -; . HOGENOM HBG673855; -; . OMA CIALVLP; -; . ProtClustDB PRK10002; -; . BioCyc EcoCyc:EG10671-MONOMER; -; . Genevestigator P02931; -; . GO GO:0009279; C:cell outer membrane; IDA:EcoCyc; . GO GO:0005886; C:plasma membrane; IEA:UniProtKB-KW; . GO GO:0046930; C:pore complex; IDA:EcoCyc; . GO GO:0042912; F:colicin transmembrane transporter activity; IMP:EcoCyc; . GO GO:0015238; F:drug transmembrane transporter activity; IMP:EcoCyc; . GO GO:0015075; F:ion transmembrane transporter activity; TAS:EcoCyc; . GO GO:0015288; F:porin activity; IMP:EcoliWiki; . InterPro IPR023614; Porin_dom; . InterPro IPR001897; Porin_gammaproteobac; . InterPro IPR001702; Porin_Gram-ve; . InterPro IPR013793; Porin_Gram-ve_CS; . Gene3D G3DSA:2.40.160.10; Porin_Gram-ve; 1; . Pfam PF00267; Porin_1; 1; . PRINTS PR00183; ECOLIPORIN; . PRINTS PR00182; ECOLNEIPORIN; . PROSITE PS00576; GRAM_NEG_PORIN; 1; . SWISS-2DPAGE (search AC)