resource logo

SWISS-2DPAGE

Attention: World-2DPAGE is no longer maintained.


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P09103




SWISS-2DPAGE:  P09103


P09103


General information about the entry
View entry in simple text format
Entry namePDIA1_MOUSE
Primary accession numberP09103
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Endoplasmic reticulum resident protein 59; Short=ER protein 59; Short=ERp59; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor;.
Gene nameName=P4hb
Synonyms=Pdia1
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, Expasy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

ISLETS_MOUSE {Pancreatic islet cells}
Mus musculus (Mouse)
Tissue: Pancreatic islet
ISLETS_MOUSE
  map experimental info
  protein estimated location
 
ISLETS_MOUSE

MAP LOCATIONS:
pI=4.96; Mw=62422  [identification data]
pI=4.91; Mw=57522
pI=4.92; Mw=57048
pI=4.94; Mw=57048  [identification data]

MAPPING (identification):
SPOTS 2D-0018EK:, 2D-0018IS: Peptide mass fingerprinting [2]; SPOTS 2D-001*: MATCHING WITH THE MOUSE LIVER MASTER GEL [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=4.86; Mw=55474  [identification data]
pI=4.82; Mw=55784  [identification data]
pI=4.88; Mw=55474  [identification data]

MAPPING (identification):
MATCHING WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1], MICROSEQUENCING AND MASS FINGERPRINTING [2].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=4.77; Mw=57681
pI=4.83; Mw=57205

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=4.91; Mw=55685
pI=4.93; Mw=55510

MAPPING (identification):
MATCHING WITH THE MOUSE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
COMPLUYEAST-2DPAGEP09103; PDIA1_MOUSE.
REPRODUCTION-2DPAGEP09103; P09103.
UniProtKB/Swiss-ProtP09103; PDIA1_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePDIA1_MOUSE
Primary accession numberP09103
Secondary accession number(s) Q922C8
Sequence was last modified on July 27, 2011 (version 2)
Annotations were last modified on September 21, 2011 (version 120)
Name and origin of the protein
DescriptionRecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Endoplasmic reticulum resident protein 59; Short=ER protein 59; Short=ERp59; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor;
Gene nameName=P4hb
Synonyms=Pdia1
Encoded onName=P4hb; Synonyms=Pdia1
KeywordsCell membrane; Chaperone; Complete proteome; Disulfide bond; Endoplasmic reticulum; Isomerase; Membrane; Redox-active center; Reference proteome; Repeat; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX06453; CAA29759.1; -; mRNA
EMBLJ05185; AAA39906.1; -; mRNA
EMBLAK150002; BAE29230.1; -; mRNA
EMBLAK150422; BAE29545.1; -; mRNA
EMBLAK150805; BAE29868.1; -; mRNA
EMBLAK152141; BAE30979.1; -; mRNA
EMBLAK152217; BAE31045.1; -; mRNA
EMBLAK159606; BAE35225.1; -; mRNA
EMBLAK159965; BAE35520.1; -; mRNA
EMBLAK168330; BAE40268.1; -; mRNA
EMBLAK168893; BAE40710.1; -; mRNA
EMBLAK170603; BAE41907.1; -; mRNA
EMBLAL663030; CAM27084.1; -; Genomic_DNA
EMBLBC008549; AAH08549.1; -; mRNA
EMBLBC093512; AAH93512.1; -; mRNA
IPIIPI00133522; -; .
PIRA34930; ISMSSS; .
RefSeqNP_035162.1; NM_011032.2; .
UniGeneMm.16660; -; .
UniGeneMm.392196; -; .
HSSPP07237; 1MEK; .
ProteinModelPortalP09103; -; .
IntActP09103; 4; .
MINTMINT-1177082; -; .
STRINGP09103; -; .
PhosphoSiteP09103; -; .
SWISS-2DPAGEP09103; -; .
COMPLUYEAST-2DPAGEP09103; -; .
REPRODUCTION-2DPAGEP09103; -; .
PRIDEP09103; -; .
EnsemblENSMUST00000026122; ENSMUSP00000026122; ENSMUSG00000025130; .
GeneID18453; -; .
KEGGmmu:18453; -; .
CTD5034; -; .
MGIMGI:97464; P4hb; .
eggNOGroNOG08939; -; .
HOGENOMHBG627841; -; .
HOVERGENHBG005920; -; .
InParanoidP09103; -; .
OrthoDBEOG4JWVDB; -; .
PhylomeDBP09103; -; .
ArrayExpressP09103; -; .
BgeeP09103; -; .
CleanExMM_P4HB; -; .
GenevestigatorP09103; -; .
GermOnlineENSMUSG00000025130; Mus musculus; .
GOGO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0005792; C:microsome; IDA:MGI; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0009055; F:electron carrier activity; IEA:InterPro; .
GOGO:0003756; F:protein disulfide isomerase activity; IEA:EC; .
GOGO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro; .
GOGO:0045454; P:cell redox homeostasis; IEA:InterPro; .
GOGO:0006662; P:glycerol ether metabolic process; IEA:InterPro; .
InterProIPR005788; Disulphide_isomerase; .
InterProIPR005792; Prot_disulphide_isomerase; .
InterProIPR005746; Thioredoxin; .
InterProIPR012336; Thioredoxin-like_fold; .
InterProIPR017937; Thioredoxin_CS; .
InterProIPR013766; Thioredoxin_domain; .
Gene3DG3DSA:3.40.30.10; Thioredoxin_fold; 3; .
PfamPF00085; Thioredoxin; 2; .
PRINTSPR00421; THIOREDOXIN; .
SUPFAMSSF52833; Thiordxn-like_fd; 4; .
TIGRFAMsTIGR01130; ER_PDI_fam; 1; .
TIGRFAMsTIGR01126; Pdi_dom; 2; .
PROSITEPS00014; ER_TARGET; 1; .
PROSITEPS00194; THIOREDOXIN_1; 2; .
PROSITEPS51352; THIOREDOXIN_2; 2; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server