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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P0A6H5 SWISS-2DPAGE:  P0A6H5 P0A6H5 General information about the entry View entry in simple text format Entry name HSLU_ECOLI Primary accession number P0A6H5 Secondary accession number(s) P32168 integrated into SWISS-2DPAGE on  August 1, 1995 (release 2) 2D Annotations were last modified on May 15, 2003 (version 1) General Annotations were last modified on  May 19, 2011 (version 13) Name and origin of the protein Description RecName: Full=ATP-dependent protease ATPase subunit HslU; AltName: Full=Heat shock protein HslU; AltName: Full=Unfoldase HslU;. Gene name Name=hslU Synonyms=htpI OrderedLocusNames=b3931, JW3902 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996) [2]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996) [3]   PROTEIN EXPRESSION MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan] Gage D.J., Neidhardt F.C. ''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';'' J. Bacteriol. 175(1):7105-7108(1993) 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000KTK: pI=5.29; Mw=48676 EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [3]. MAPPING (identification): GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references ECO2DBASE D048.5; 6TH EDITION. UniProtKB/Swiss-Prot P0A6H5; HSLU_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name HSLU_ECOLI Primary accession number P0A6H5 Secondary accession number(s) P32168 Q2M8M7 Sequence was last modified on  March 29, 2005 (version 1) Annotations were last modified on  October 19, 2011 (version 68) Name and origin of the protein Description RecName: Full=ATP-dependent protease ATPase subunit HslU; AltName: Full=Heat shock protein HslU; AltName: Full=Unfoldase HslU; Gene name Name=hslU Synonyms=htpI OrderedLocusNames=b3931, JW3902 Encoded on Name=hslU; Synonyms=htpI; OrderedLocusNames=b3931, JW3902 Keywords 3D-structure; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Nucleotide-binding; Reference proteome; Stress response. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL L19201; AAB03063.1; -; Genomic_DNA EMBL U00096; AAC76913.1; -; Genomic_DNA EMBL AP009048; BAE77379.1; -; Genomic_DNA PIR JT0761; JT0761; . RefSeq NP_418366.1; NC_000913.2; . PDB 1DO0; X-ray; 3.00 A; A/B/C/D/E/F=2-443 PDB 1DO2; X-ray; 4.00 A; A/B/C/D=2-443 PDB 1E94; X-ray; 2.80 A; E/F=2-443 PDB 1G4A; X-ray; 3.00 A; E/F=1-443 PDB 1G4B; X-ray; 7.00 A; E/F/K/L=1-443 PDB 1HQY; X-ray; 2.80 A; E/F=2-443 PDB 1HT1; X-ray; 2.80 A; E/F/G/I=2-443 PDB 1HT2; X-ray; 2.80 A; E/F/G/H=2-443 PDB 1YYF; X-ray; 4.16 A; A/B=1-443 PDBsum 1DO0; -; . PDBsum 1DO2; -; . PDBsum 1E94; -; . PDBsum 1G4A; -; . PDBsum 1G4B; -; . PDBsum 1HQY; -; . PDBsum 1HT1; -; . PDBsum 1HT2; -; . PDBsum 1YYF; -; . ProteinModelPortal P0A6H5; -; . SMR P0A6H5; 1-443; . DIP DIP-31855N; -; . IntAct P0A6H5; 48; . SWISS-2DPAGE P0A6H5; -; . ECO2DBASE D048.5; 6TH EDITION; . EnsemblBacteria EBESCT00000003987; EBESCP00000003987; EBESCG00000003262; . EnsemblBacteria EBESCT00000018172; EBESCP00000017463; EBESCG00000017227; . GeneID 948430; -; . GenomeReviews AP009048_GR; JW3902; . GenomeReviews U00096_GR; b3931; . KEGG ecj:JW3902; -; . KEGG eco:b3931; -; . EchoBASE EB1827; -; . EcoGene EG11881; hslU; . eggNOG COG1220; -; . GeneTree EBGT00050000010205; -; . HOGENOM HBG745965; -; . OMA EASKFTE; -; . ProtClustDB PRK05201; -; . BioCyc EcoCyc:EG11881-MONOMER; -; . Genevestigator P0A6H5; -; . GO GO:0009376; C:HslUV protease complex; IEA:InterPro; . GO GO:0016020; C:membrane; IDA:UniProtKB; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0016887; F:ATPase activity; IEA:InterPro; . GO GO:0070011; F:peptidase activity; acting on L-amino acid peptides; IEA:InterPro GO GO:0005515; F:protein binding; IPI:IntAct; . GO GO:0009408; P:response to heat; IEP:EcoliWiki; . HAMAP MF_00249; HslU; 1; - InterPro IPR003593; ATPase_AAA+_core; . InterPro IPR013093; ATPase_AAA-2; . InterPro IPR003959; ATPase_AAA_core; . InterPro IPR019489; Clp_ATPase_C; . InterPro IPR004491; HslU; . PANTHER PTHR11262:SF3; HslU; 1; . Pfam PF00004; AAA; 1; . Pfam PF07724; AAA_2; 1; . Pfam PF10431; ClpB_D2-small; 1; . SMART SM00382; AAA; 1; . TIGRFAMs TIGR00390; HslU; 1; . SWISS-2DPAGE (search AC)