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SWISS-2DPAGE

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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AD96




SWISS-2DPAGE:  P0AD96


P0AD96


General information about the entry
View entry in simple text format
Entry nameLIVJ_ECOLI
Primary accession numberP0AD96
Secondary accession number(s) P02917
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 6)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Leu/Ile/Val-binding protein; Short=LIV-BP; Flags: Precursor;.
Gene nameName=livJ
OrderedLocusNames=b3460, JW3425
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, Expasy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
[3]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[4]   PROTEIN EXPRESSION
MEDLINE=92138602; PubMed=1346534; [NCBI, Expasy, EBI, Israel, Japan]
Ernsting B.R., Atkinson M.R., Ninfa A.J., Matthews R.G.
''''''Characterization of the regulon controlled by the leucine-responsive regulatory protein in Escherichia coli'';'';''
J. Bacteriol. 174(1):1109-1118(1992)
[5]   PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
[6]   PROTEIN EXPRESSION
MEDLINE=93352420; PubMed=8349554; [NCBI, Expasy, EBI, Israel, Japan]
Sankar P., Hutton M.E., Vanbogelen R.A., Clark R.L., Neidhardt F.C.
''''''Expression analysis of cloned chromosomal segments of Escherichia coli'';'';''
J. Bacteriol. 175(1):5145-5152(1993)
[7]   MAPPING ON GEL
PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[8]   MAPPING ON GEL
PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.28; Mw=42043

EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [5]; IS REDUCED IN A LRP+ STRAIN WHEN LEUCINE IS ADDED TO THE MEDIUM [4]; WAS ENCODED AND EXPRESSED FROM PLASMID PFNK613L [6].

MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY BLOCH [2] AND VANBOGELEN [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.31; Mw=40301  [identification data]
pI=5.21; Mw=16972

MAPPING (identification):
Peptide mass fingerprinting and microsequencing (EDIKV) [7].



ECOLI5.5-6.7 {Escherichia coli(5.5-6.7)}
Escherichia coli
ECOLI5.5-6.7
  map experimental info
  protein estimated location
 
ECOLI5.5-6.7

MAP LOCATIONS:
pI=5.73; Mw=30106

MAPPING (identification):
GEL MATCHING WITH ECOLI5-6 [7].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.09; Mw=50544  [identification data]
pI=5.23; Mw=50424  [identification data]
pI=5.67; Mw=30753  [identification data]
pI=5.33; Mw=23392  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [7].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.18; Mw=39439  [identification data]
pI=5.30; Mw=38773  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [8].

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [8].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP0AD96; LIVJ_ECOLI.
ECO2DBASED040.7; 6TH EDITION.
UniProtKB/Swiss-ProtP0AD96; LIVJ_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameLIVJ_ECOLI
Primary accession numberP0AD96
Secondary accession number(s) P02917 P76698 Q2M7C3 Q8CVL7 Q8X6S2
Sequence was last modified on December 6, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 52)
Name and origin of the protein
DescriptionRecName: Full=Leu/Ile/Val-binding protein; Short=LIV-BP; Flags: Precursor;
Gene nameName=livJ
OrderedLocusNames=b3460, JW3425
Encoded onName=livJ; OrderedLocusNames=b3460, JW3425
Keywords3D-structure; Amino-acid transport; Complete proteome; Direct protein sequencing; Disulfide bond; Periplasm; Reference proteome; Signal; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ05516; AAA83881.1; -; Genomic_DNA
EMBLM29377; AAA24075.1; -; Genomic_DNA
EMBLU00039; AAB18435.1; ALT_INIT; Genomic_DNA
EMBLU00096; AAT48185.1; -; Genomic_DNA
EMBLAP009048; BAE77833.1; -; Genomic_DNA
PIRG65142; BLEC; .
RefSeqYP_026223.1; NC_000913.2; .
PDB1Z15; X-ray; 1.70 A; A=24-367
PDB1Z16; X-ray; 1.72 A; A=24-367
PDB1Z17; X-ray; 1.96 A; A=24-367
PDB1Z18; X-ray; 2.10 A; A=24-367
PDB2LIV; X-ray; 2.40 A; A=24-367
PDBsum1Z15; -; .
PDBsum1Z16; -; .
PDBsum1Z17; -; .
PDBsum1Z18; -; .
PDBsum2LIV; -; .
ProteinModelPortalP0AD96; -; .
SMRP0AD96; 24-367; .
IntActP0AD96; 4; .
TCDB3.A.1.4.1; ATP-binding cassette (ABC) superfamily; .
SWISS-2DPAGEP0AD96; -; .
2DBase-EcoliP0AD96; -; .
ECO2DBASED040.7; 6TH EDITION; .
EnsemblBacteriaEBESCT00000002981; EBESCP00000002981; EBESCG00000002441; .
EnsemblBacteriaEBESCT00000016320; EBESCP00000015611; EBESCG00000015380; .
GeneID947971; -; .
GenomeReviewsAP009048_GR; JW3425; .
GenomeReviewsU00096_GR; b3460; .
KEGGecj:JW3425; -; .
KEGGeco:b3460; -; .
EchoBASEEB0534; -; .
EcoGeneEG10539; livJ; .
eggNOGCOG0683; -; .
GeneTreeEBGT00050000010005; -; .
HOGENOMHBG695960; -; .
OMAMGILRMN; -; .
ProtClustDBPRK15404; -; .
BioCycEcoCyc:LIVJ-MONOMER; -; .
GenevestigatorP0AD96; -; .
GOGO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro; .
GOGO:0006865; P:amino acid transport; IEA:UniProtKB-KW; .
InterProIPR001828; ANF_lig-bd_rcpt; .
InterProIPR000709; Leu_Ile_Val-bd; .
PfamPF01094; ANF_receptor; 1; .
PRINTSPR00337; LEUILEVALBP; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server