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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P0AE18 SWISS-2DPAGE:  P0AE18 P0AE18 General information about the entry View entry in simple text format Entry name AMPM_ECOLI Primary accession number P0AE18 Secondary accession number(s) P07906 integrated into SWISS-2DPAGE on  August 1, 1995 (release 2) 2D Annotations were last modified on May 15, 2003 (version 3) General Annotations were last modified on  May 19, 2011 (version 10) Name and origin of the protein Description RecName: Full=Methionine aminopeptidase; Short=MAP; EC=3.4.11.18; AltName: Full=Peptidase M;. Gene name Name=map OrderedLocusNames=b0168, JW0163 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996) [2]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996) [3]   MAPPING ON GEL PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan] Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C. ''''''New perspectives in the Escherichia coli proteome investigation'';'';'' Proteomics 1(1):409-423(2001) Comments SUBUNIT: MONOMER 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000L7N: pI=5.66; Mw=33904 MAPPING (identification): GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2]. ECOLI5.5-6.7 {Escherichia coli(5.5-6.7)} Escherichia coli   map experimental info   protein estimated location   ECOLI5.5-6.7 MAP LOCATIONS: SPOT 2D-001UCG: pI=5.81; Mw=37872 MAPPING (identification): GEL MATCHING WITH ECOLI5-6 [3]. ECOLI5-6 {Escherichia coli(5-6)} Escherichia coli   map experimental info   protein estimated location   ECOLI5-6 MAP LOCATIONS: SPOT 2D-001LBR: pI=5.71; Mw=38478   [identification data] MAPPING (identification): Peptide mass fingerprinting [3]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references ECO2DBASE F029.7; 6TH EDITION. UniProtKB/Swiss-Prot P0AE18; AMPM_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name AMPM_ECOLI Primary accession number P0AE18 Secondary accession number(s) P07906 Sequence was last modified on  August 1, 1988 (version 1) Annotations were last modified on  October 19, 2011 (version 64) Name and origin of the protein Description RecName: Full=Methionine aminopeptidase; Short=MAP; EC=3.4.11.18; AltName: Full=Peptidase M; Gene name Name=map OrderedLocusNames=b0168, JW0163 Encoded on Name=map; OrderedLocusNames=b0168, JW0163 Keywords 3D-structure; Aminopeptidase; Cobalt; Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Protease; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M15106; AAA24112.1; -; Genomic_DNA EMBL U70214; AAB08597.1; -; Genomic_DNA EMBL U00096; AAC73279.1; -; Genomic_DNA EMBL AP009048; BAB96743.1; -; Genomic_DNA PIR A27761; DPECM; . RefSeq NP_414710.1; NC_000913.2; . PDB 1C21; X-ray; 1.80 A; A=2-264 PDB 1C22; X-ray; 1.75 A; A=2-264 PDB 1C23; X-ray; 2.00 A; A=2-264 PDB 1C24; X-ray; 1.70 A; A=2-264 PDB 1C27; X-ray; 1.95 A; A=2-264 PDB 1MAT; X-ray; 2.40 A; A=1-264 PDB 1XNZ; X-ray; 1.52 A; A=1-264 PDB 1YVM; X-ray; 1.60 A; A=1-264 PDB 2BB7; X-ray; 1.70 A; A=1-264 PDB 2EVC; X-ray; 1.60 A; A=1-264 PDB 2EVM; X-ray; 1.70 A; A=1-264 PDB 2EVO; X-ray; 1.70 A; A/B=1-264 PDB 2GG0; X-ray; 1.28 A; A=2-264 PDB 2GG2; X-ray; 1.00 A; A=2-264 PDB 2GG3; X-ray; 1.45 A; A=2-264 PDB 2GG5; X-ray; 2.12 A; A=2-264 PDB 2GG7; X-ray; 1.12 A; A=2-264 PDB 2GG8; X-ray; 1.80 A; A=2-264 PDB 2GG9; X-ray; 1.05 A; A=2-264 PDB 2GGB; X-ray; 2.13 A; A=2-264 PDB 2GGC; X-ray; 1.00 A; A=2-264 PDB 2GTX; X-ray; 2.00 A; A/B=4-264 PDB 2GU4; X-ray; 1.80 A; A/B=4-264 PDB 2GU5; X-ray; 1.60 A; A/B=4-264 PDB 2GU6; X-ray; 1.70 A; A/B=4-264 PDB 2GU7; X-ray; 2.00 A; A/B=4-264 PDB 2MAT; X-ray; 1.90 A; A=1-264 PDB 2P98; X-ray; 1.70 A; A=2-263 PDB 2P99; X-ray; 1.80 A; A=2-262 PDB 2P9A; X-ray; 1.60 A; A=2-263 PDB 2Q92; X-ray; 1.90 A; A=2-263 PDB 2Q93; X-ray; 1.60 A; A=2-264 PDB 2Q94; X-ray; 1.63 A; A=2-263 PDB 2Q95; X-ray; 1.70 A; A=2-264 PDB 2Q96; X-ray; 1.60 A; A=2-264 PDB 3D27; X-ray; 2.20 A; A=4-264 PDB 3MAT; X-ray; 2.00 A; A=1-264 PDB 4MAT; X-ray; 2.00 A; A=1-264 PDBsum 1C21; -; . PDBsum 1C22; -; . PDBsum 1C23; -; . PDBsum 1C24; -; . PDBsum 1C27; -; . PDBsum 1MAT; -; . PDBsum 1XNZ; -; . PDBsum 1YVM; -; . PDBsum 2BB7; -; . PDBsum 2EVC; -; . PDBsum 2EVM; -; . PDBsum 2EVO; -; . PDBsum 2GG0; -; . PDBsum 2GG2; -; . PDBsum 2GG3; -; . PDBsum 2GG5; -; . PDBsum 2GG7; -; . PDBsum 2GG8; -; . PDBsum 2GG9; -; . PDBsum 2GGB; -; . PDBsum 2GGC; -; . PDBsum 2GTX; -; . PDBsum 2GU4; -; . PDBsum 2GU5; -; . PDBsum 2GU6; -; . PDBsum 2GU7; -; . PDBsum 2MAT; -; . PDBsum 2P98; -; . PDBsum 2P99; -; . PDBsum 2P9A; -; . PDBsum 2Q92; -; . PDBsum 2Q93; -; . PDBsum 2Q94; -; . PDBsum 2Q95; -; . PDBsum 2Q96; -; . PDBsum 3D27; -; . PDBsum 3MAT; -; . PDBsum 4MAT; -; . ProteinModelPortal P0AE18; -; . SMR P0AE18; 2-264; . DIP DIP-48040N; -; . IntAct P0AE18; 25; . MINT MINT-1219572; -; . MEROPS M24.001; -; . SWISS-2DPAGE P0AE18; -; . ECO2DBASE F029.7; 6TH EDITION; . EnsemblBacteria EBESCT00000000850; EBESCP00000000850; EBESCG00000000708; . EnsemblBacteria EBESCT00000015484; EBESCP00000014775; EBESCG00000014544; . GeneID 947882; -; . GenomeReviews AP009048_GR; JW0163; . GenomeReviews U00096_GR; b0168; . KEGG ecj:JW0163; -; . KEGG eco:b0168; -; . EchoBASE EB0565; -; . EcoGene EG10570; map; . eggNOG COG0024; -; . GeneTree EBGT00050000010664; -; . HOGENOM HBG299384; -; . OMA ENGCEIM; -; . ProtClustDB PRK05716; -; . BioCyc EcoCyc:EG10570-MONOMER; -; . BioCyc MetaCyc:EG10570-MONOMER; -; . BindingDB P0AE18; -; . Genevestigator P0AE18; -; . GO GO:0008198; F:ferrous iron binding; IDA:EcoCyc; . GO GO:0070006; F:metalloaminopeptidase activity; IDA:EcoCyc; . GO GO:0070084; P:protein initiator methionine removal; IDA:EcoCyc; . GO GO:0006508; P:proteolysis; IEA:InterPro; . InterPro IPR001714; Pept_M24_MAP; . InterPro IPR000994; Pept_M24_structural-domain; . InterPro IPR002467; Pept_M24A_MAP1; . Gene3D G3DSA:3.90.230.10; Peptidase_M24_cat_core; 1; . Pfam PF00557; Peptidase_M24; 1; . PRINTS PR00599; MAPEPTIDASE; . SUPFAM SSF55920; Peptidase_M24_cat_core; 1; . TIGRFAMs TIGR00500; Met_pdase_I; 1; . PROSITE PS00680; MAP_1; 1; . SWISS-2DPAGE (search AC)