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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P0AGJ9 SWISS-2DPAGE:  P0AGJ9 P0AGJ9 General information about the entry View entry in simple text format Entry name SYY_ECOLI Primary accession number P0AGJ9 Secondary accession number(s) P00951 integrated into SWISS-2DPAGE on  August 1, 1995 (release 2) 2D Annotations were last modified on November 9, 2001 (version 1) General Annotations were last modified on  May 19, 2011 (version 12) Name and origin of the protein Description RecName: Full=Tyrosyl-tRNA synthetase; EC=6.1.1.1; AltName: Full=Tyrosine--tRNA ligase; Short=TyrRS;. Gene name Name=tyrS OrderedLocusNames=b1637, JW1629 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996) [2]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996) [3]   MAPPING ON GEL MEDLINE=81069671; PubMed=7002901; [NCBI, Expasy, EBI, Israel, Japan] Phillips T.A., Bloch P.L., Neidhardt F.C. ''''''Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins'';'';'' J. Bacteriol. 144(1):1024-1033(1980) Comments SUBUNIT: HOMODIMER 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000KX3: pI=5.59; Mw=44615 MAPPING (identification): GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND PHILLIPS [3]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references ECO2DBASE G044.0; 6TH EDITION. UniProtKB/Swiss-Prot P0AGJ9; SYY_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name SYY_ECOLI Primary accession number P0AGJ9 Secondary accession number(s) P00951 Sequence was last modified on  January 23, 2007 (version 2) Annotations were last modified on  October 19, 2011 (version 62) Name and origin of the protein Description RecName: Full=Tyrosyl-tRNA synthetase; EC=6.1.1.1; AltName: Full=Tyrosine--tRNA ligase; Short=TyrRS; Gene name Name=tyrS OrderedLocusNames=b1637, JW1629 Encoded on Name=tyrS; OrderedLocusNames=b1637, JW1629 Keywords 3D-structure; Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL J01719; AAA24707.1; -; Genomic_DNA EMBL U00096; AAC74709.1; -; Genomic_DNA EMBL AP009048; BAA15398.1; -; Genomic_DNA EMBL M92351; AAA24710.1; -; Genomic_DNA PIR A01178; SYECYT; . RefSeq NP_416154.1; NC_000913.2; . PDB 1VBM; X-ray; 2.70 A; A/B=5-322 PDB 1VBN; X-ray; 2.70 A; A/B=5-322 PDB 1WQ3; X-ray; 2.00 A; A=1-322 PDB 1WQ4; X-ray; 2.00 A; A=2-322 PDB 1X8X; X-ray; 2.00 A; A=1-322 PDB 2YXN; X-ray; 1.80 A; A=1-322 PDBsum 1VBM; -; . PDBsum 1VBN; -; . PDBsum 1WQ3; -; . PDBsum 1WQ4; -; . PDBsum 1X8X; -; . PDBsum 2YXN; -; . ProteinModelPortal P0AGJ9; -; . SMR P0AGJ9; 1-423; . DIP DIP-36227N; -; . IntAct P0AGJ9; 8; . MINT MINT-1224558; -; . SWISS-2DPAGE P0AGJ9; -; . ECO2DBASE G044.0; 6TH EDITION; . EnsemblBacteria EBESCT00000001106; EBESCP00000001106; EBESCG00000000919; . EnsemblBacteria EBESCT00000001107; EBESCP00000001107; EBESCG00000000919; . EnsemblBacteria EBESCT00000016356; EBESCP00000015647; EBESCG00000015416; . GeneID 948855; -; . GenomeReviews AP009048_GR; JW1629; . GenomeReviews U00096_GR; b1637; . KEGG ecj:JW1629; -; . KEGG eco:b1637; -; . EchoBASE EB1036; -; . EcoGene EG11043; tyrS; . eggNOG COG0162; -; . GeneTree EBGT00050000010726; -; . HOGENOM HBG288125; -; . OMA TFYIGFD; -; . ProtClustDB PRK05912; -; . BioCyc EcoCyc:TYRS-MONOMER; -; . BioCyc MetaCyc:TYRS-MONOMER; -; . Genevestigator P0AGJ9; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0016020; C:membrane; IDA:UniProtKB; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0003723; F:RNA binding; IEA:UniProtKB-KW; . GO GO:0004831; F:tyrosine-tRNA ligase activity; IEA:EC; . GO GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro; . HAMAP MF_02006; Tyr_tRNA_synth_type1; 1; - InterPro IPR001412; aa-tRNA-synth_I_CS; . InterPro IPR002305; aa-tRNA-synth_Ic; . InterPro IPR014729; Rossmann-like_a/b/a_fold; . InterPro IPR002942; S4_RNA-bd; . InterPro IPR002307; Tyr-tRNA-synth; . InterPro IPR024088; Tyr-tRNA-synth_bac-type; . InterPro IPR024107; Tyr-tRNA-synth_bac_1; . Gene3D G3DSA:3.10.290.10; G3DSA:3.10.290.10; 1; . Gene3D G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1; . PANTHER PTHR11766; Tyr_tRNA-synt_1b; 1; . Pfam PF01479; S4; 1; . Pfam PF00579; tRNA-synt_1b; 1; . PRINTS PR01040; TRNASYNTHTYR; . SMART SM00363; S4; 1; . TIGRFAMs TIGR00234; TyrS; 1; . PROSITE PS00178; AA_TRNA_LIGASE_I; 1; . PROSITE PS50889; S4; 1; . SWISS-2DPAGE (search AC)