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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P18859




SWISS-2DPAGE:  P18859


P18859


General information about the entry
View entry in simple text format
Entry nameATP5J_HUMAN
Primary accession numberP18859
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onOctober 1, 2001 (version 3)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase-coupling factor 6, mitochondrial; Short=ATPase subunit F6; Flags: Precursor;.
Gene nameName=ATP5J
Synonyms=ATP5A, ATPM
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, Expasy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[4]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
Comments
  • SUBUNIT: ONE OF THE CHAINS OF THE NONENZYMATIC COMPONENT OF THE MITOCHONDRIAL ATPASE COMPLEX
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=5.31; Mw=11038

MAPPING (identification):
MICROSEQUENCING [3].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=5.35; Mw=9218

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [4].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=5.37; Mw=9810

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.33; Mw=10200

MAPPING (identification):
MICROSEQUENCING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP18859; ATP5J_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameATP5J_HUMAN
Primary accession numberP18859
Sequence was last modified on November 1, 1990 (version 1)
Annotations were last modified on October 19, 2011 (version 120)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase-coupling factor 6, mitochondrial; Short=ATPase subunit F6; Flags: Precursor;
Gene nameName=ATP5J
Synonyms=ATP5A, ATPM
Encoded onName=ATP5J; Synonyms=ATP5A, ATPM
KeywordsAcetylation; CF(0); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transit peptide; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM37104; AAA51807.1; -; mRNA
EMBLM73031; AAA58630.1; -; mRNA
EMBLAL110183; CAB53667.1; -; mRNA
EMBLBT007244; AAP35908.1; -; mRNA
EMBLAP001694; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLBC001178; AAH01178.1; -; mRNA
IPIIPI00002521; -; .
PIRJT0563; JT0563; .
RefSeqNP_001003696.1; NM_001003696.1; .
RefSeqNP_001003697.1; NM_001003697.1; .
RefSeqNP_001003701.1; NM_001003701.1; .
RefSeqNP_001003703.1; NM_001003703.1; .
RefSeqNP_001676.2; NM_001685.4; .
UniGeneHs.246310; -; .
ProteinModelPortalP18859; -; .
SMRP18859; 33-108; .
IntActP18859; 2; .
STRINGP18859; -; .
PhosphoSiteP18859; -; .
SWISS-2DPAGEP18859; -; .
PRIDEP18859; -; .
EnsemblENST00000284971; ENSP00000284971; ENSG00000154723; .
EnsemblENST00000400087; ENSP00000382959; ENSG00000154723; .
EnsemblENST00000400089; ENSP00000382961; ENSG00000154723; .
EnsemblENST00000400090; ENSP00000382962; ENSG00000154723; .
EnsemblENST00000400093; ENSP00000382965; ENSG00000154723; .
EnsemblENST00000400094; ENSP00000382966; ENSG00000154723; .
EnsemblENST00000457143; ENSP00000389649; ENSG00000154723; .
GeneID522; -; .
KEGGhsa:522; -; .
UCSCuc002yls.1; human; .
CTD522; -; .
GeneCardsGC21M012499; -; .
H-InvDBHIX0016040; -; .
HGNCHGNC:847; ATP5J; .
HPAHPA031069; -; .
MIM603152; gene; .
neXtProtNX_P18859; -; .
PharmGKBPA25137; -; .
eggNOGprNOG20632; -; .
HOVERGENHBG062261; -; .
PhylomeDBP18859; -; .
Pathway_Interaction_DBhnf3apathway; FOXA1 transcription factor network; .
ReactomeREACT_6305; Respiratory electron transport; ATP synthesis by chemiosmotic coupling; and heat production by uncoupling proteins
NextBio2163; -; .
ArrayExpressP18859; -; .
BgeeP18859; -; .
CleanExHS_ATP5J; -; .
GenevestigatorP18859; -; .
GermOnlineENSG00000154723; Homo sapiens; .
GOGO:0015078; F:hydrogen ion transmembrane transporter activity; IEA:InterPro; .
GOGO:0006200; P:ATP catabolic process; IDA:GOC; .
GOGO:0042776; P:mitochondrial ATP synthesis coupled proton transport; IC:UniProtKB; .
GOGO:0022904; P:respiratory electron transport chain; TAS:Reactome; .
InterProIPR008387; ATPase_F0-cplx_f6su_mt; .
InterProIPR016349; ATPase_F0-cplx_f6su_mt_subgr; .
PANTHERPTHR12441; ATPase_F0_F6_mt; 1; .
PfamPF05511; ATP-synt_F6; 1; .
PIRSFPIRSF002455; ATP_synthase_coupling_factor_6; 1; .
SUPFAMSSF111357; ATPase_F0_F6_mt; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server