Due to maintenance work, this service will not be available Thursday August 21st between 7am and 8am CEST.
World-2DPAGE Home


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: A1AT_HUMAN




SWISS-2DPAGE:  A1AT_HUMAN


A1AT_HUMAN


General information about the entry
View entry in simple text format
Entry nameA1AT_HUMAN
Primary accession numberP01009
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 18)
Name and origin of the protein
DescriptionRecName: Full=Alpha-1-antitrypsin; AltName: Full=Alpha-1 protease inhibitor; AltName: Full=Alpha-1-antiproteinase; AltName: Full=Serpin A1; Contains: RecName: Full=Short peptide from AAT; Short=SPAAT; Flags: Precursor;.
Gene nameName=SERPINA1
Synonyms=AAT, PI
ORFNames=PRO0684, PRO2209
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   PATHOLOGICAL LEVEL
Allen R.C., Bienvenu J., Laurent P., Suskind R.M.
(IN) Robert C. Allen, et al. (eds.)Marker proteins in inflammation: proceedings of the symposium, Lyon, France, April 1981, pp.0-0, W. de Gruyter, Berlin (1982)
[3]   PATHOLOGICAL VARIANTS
Sharp H.L.
(IN) McKusick V.A., Clairbone R. (eds.)Medical genetics, pp.131-140, H. P. Publ. Co. Inc., New York (1973)
[4]   GENETIC VARIANTS
MEDLINE=80181048; PubMed=6102963; [NCBI, ExPASy, EBI, Israel, Japan]
Cox D.W., Johnson A.M., Fagerhol M.K.
''''''Report of Nomenclature Meeting for alpha 1-antitrypsin, INSERM, Rouen/Bois-Guillaume-1978'';'';''
Hum. Genet. 53(1):429-433(1980)
[5]   PATHOLOGICAL VARIANTS
MEDLINE=83297520; PubMed=6604220; [NCBI, ExPASy, EBI, Israel, Japan]
Owen M.C., Brennan S.O., Lewis J.H., Carrell R.W.
''''''Mutation of antitrypsin to antithrombin. alpha 1-antitrypsin Pittsburgh (358 Met leads to Arg), a fatal bleeding disorder'';'';''
New Engl. J. Med. 309(1):694-698(1983)
[6]   MAPPING ON GEL
MEDLINE=93092937; PubMed=1459097; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.-C., James R.W., Tissot J.-D., Bjellqvist B., Hochstrasser D.F.
''''''Plasma protein map: an update by microsequencing'';'';''
Electrophoresis 13(1):707-714(1992)
[7]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
Comments
  • SUBUNIT: SINGLE CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=4.94; Mw=132121
pI=5.00; Mw=132770
pI=5.06; Mw=130832
pI=4.78; Mw=60493
pI=4.83; Mw=59122
pI=4.90; Mw=57782
pI=4.79; Mw=58523
pI=4.96; Mw=57051
pI=5.04; Mw=56473
pI=5.08; Mw=56043
pI=5.12; Mw=57051
pI=5.15; Mw=55193

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [7].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=4.62; Mw=57821
pI=4.69; Mw=55230
pI=4.74; Mw=54511
pI=4.80; Mw=53979
pI=4.87; Mw=52240
pI=4.95; Mw=51391
pI=5.04; Mw=51223
pI=5.12; Mw=51223

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [7].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.92; Mw=56382
pI=4.97; Mw=55609
pI=5.01; Mw=54544
pI=5.08; Mw=53944

DISEASE POSITIONAL VARIANTS:
ALPHA-1-ANTITRYPSIN PITTSBURGH [5].

NORMAL POSITIONAL VARIANTS:
30 GENETIC VARIANTS KNOWN AS PI ALLELES [4].

PATHOLOGICAL LEVEL:
DECREASED DURING HEPATIC CIRRHOSIS IN CHILDREN [3] AND EMPHYSEMA; INCREASED DURING THE ACUTE-PHASE REACTION [2], LIVER DAMAGE AND PREGNANCY.

MAPPING (identification):
MATCHING WITH A PLASMA GEL [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=4.87; Mw=55663
pI=4.92; Mw=54763
pI=5.00; Mw=53878
pI=5.08; Mw=53503

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [7].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=5.01; Mw=108882
pI=5.05; Mw=106590
pI=5.11; Mw=69340
pI=4.91; Mw=57706
pI=4.87; Mw=57549
pI=4.95; Mw=56773
pI=5.00; Mw=56313
pI=4.91; Mw=56466
pI=4.87; Mw=56313
pI=5.05; Mw=55856
pI=4.91; Mw=55252
pI=4.95; Mw=54953
pI=5.00; Mw=54953
pI=5.05; Mw=54507
pI=4.95; Mw=54065
pI=4.99; Mw=53626
pI=5.10; Mw=53626
pI=5.04; Mw=53336
pI=4.87; Mw=50380
pI=4.91; Mw=49297
pI=4.95; Mw=49431
pI=4.99; Mw=48369

NORMAL LEVEL:
1900-3500 MG/L.

PATHOLOGICAL LEVEL:
DECREASED DURING HEPATIC CIRRHOSIS IN CHILDREN [3] AND EMPHYSEMA; INCREASED DURING THE ACUTE-PHASE REACTION [2], LIVER DAMAGE AND PREGNANCY.

POSITIONAL VARIANTS:
30 GENETIC VARIANTS KNOWN AS PI ALLELES [4].

MAPPING (identification):
MICROSEQUENCING [6].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP01009; P01009.
DOSAC-COBS-2DPAGEP01009; A1AT_HUMAN.
HSC-2DPAGEP01009; HUMAN.
OGP-WWWP01009.
REPRODUCTION-2DPAGEP01009; P01009.
REPRODUCTION-2DPAGEIPI00553177; IPI00553177.
Siena-2DPAGEP01009; A1AT_HUMAN.
UCD-2DPAGEP01009; A1AT_HUMAN.
UniProtKB/Swiss-ProtP01009; A1AT_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameA1AT_HUMAN
Primary accession numberP01009
Secondary accession number(s) A6PX14 B2RDQ8 Q0PVP5 Q13672 Q53XB8 Q5U0M1 Q7M4R2 Q86U18 Q86U19 Q96BF9 Q96ES1 Q9P1P0 Q9UCE6 Q9UCM3
Sequence was last modified on October 1, 1996 (version 3)
Annotations were last modified on October 19, 2011 (version 177)
Name and origin of the protein
DescriptionRecName: Full=Alpha-1-antitrypsin; AltName: Full=Alpha-1 protease inhibitor; AltName: Full=Alpha-1-antiproteinase; AltName: Full=Serpin A1; Contains: RecName: Full=Short peptide from AAT; Short=SPAAT; Flags: Precursor;
Gene nameName=SERPINA1
Synonyms=AAT, PI
ORFNames=PRO0684, PRO2209
Encoded onName=SERPINA1; Synonyms=AAT, PI; ORFNames=PRO0684, PRO2209
Keywords3D-structure; Acute phase; Alternative splicing; Blood coagulation; Complete proteome; Direct protein sequencing; Extracellular matrix; Glycoprotein; Polymorphism; Protease inhibitor; Reference proteome; Secreted; Serine protease inhibitor; Signal.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK01396; AAB59375.1; -; mRNA
EMBLK02212; AAB59495.1; -; Genomic_DNA
EMBLX01683; CAA25838.1; -; mRNA
EMBLM11465; AAA51546.1; -; mRNA
EMBLJ02619; AAA51547.1; -; Genomic_DNA
EMBLDQ682455; ABG73380.1; -; mRNA
EMBLAM048838; CAJ15161.1; -; Genomic_DNA
EMBLAF113676; AAF29581.1; -; mRNA
EMBLAF130068; AAG35496.1; -; mRNA
EMBLBX161449; CAD61914.1; -; mRNA
EMBLBX247968; CAD62306.1; -; mRNA
EMBLBX248002; CAD62334.1; ALT_INIT; mRNA
EMBLBX248257; CAD62585.1; ALT_INIT; mRNA
EMBLAK315637; BAG38005.1; -; mRNA
EMBLBT019455; AAV38262.1; -; mRNA
EMBLBC011991; AAH11991.1; -; mRNA
EMBLBC015642; AAH15642.1; -; mRNA
EMBLJ00064; AAB59369.1; -; Genomic_DNA
EMBLJ00066; AAB59370.1; -; Genomic_DNA
EMBLJ00065; AAB59370.1; JOINED; Genomic_DNA
EMBLJ00067; AAB59371.1; -; Genomic_DNA
EMBLX02920; CAA26677.1; -; mRNA
EMBLV00496; CAA23755.1; -; mRNA
EMBLM26123; AAA51545.1; -; mRNA
IPIIPI00553177; -; .
IPIIPI00790784; -; .
IPIIPI00869004; -; .
PIRA21853; ITHU; .
PIRA61391; A61391; .
RefSeqNP_000286.3; NM_000295.4; .
RefSeqNP_001002235.1; NM_001002235.2; .
RefSeqNP_001002236.1; NM_001002236.2; .
RefSeqNP_001121172.1; NM_001127700.1; .
RefSeqNP_001121173.1; NM_001127701.1; .
RefSeqNP_001121174.1; NM_001127702.1; .
RefSeqNP_001121175.1; NM_001127703.1; .
RefSeqNP_001121176.1; NM_001127704.1; .
RefSeqNP_001121177.1; NM_001127705.1; .
RefSeqNP_001121178.1; NM_001127706.1; .
RefSeqNP_001121179.1; NM_001127707.1; .
UniGeneHs.525557; -; .
PDB1ATU; X-ray; 2.70 A; A=45-418
PDB1D5S; X-ray; 3.00 A; A=44-377
B=378-418PDB; 1EZX; X-ray; 2.60 A
A=48-382B=383-418; PDB; 1HP7; X-ray
2.10 AA=25-418; PDB; 1IZ2; X-ray
2.20 AA=25-418; PDB; 1KCT; X-ray
3.46 AA=25-418; PDB; 1OO8; X-ray
2.65 AA=26-418; PDB; 1OPH; X-ray
2.30 AA=26-418; PDB; 1PSI; X-ray
2.92 AA=26-418; PDB; 1QLP; X-ray
2.00 AA=26-418; PDB; 1QMB; X-ray
2.60 AA=49-376; B=377-418; PDB; 2D26
X-ray3.30 A; A=26-382; B=383-418; PDB
2QUGX-ray; 2.00 A; A=25-418; PDB
3CWLX-ray; 2.44 A; A=25-418; PDB
3CWMX-ray; 2.51 A; A=25-418; PDB
3DRMX-ray; 2.20 A; A=26-418; PDB
3DRUX-ray; 3.20 A; A/B/C=26-418; PDB
3NDDX-ray; 1.50 A; A=46-372; B=383-418
PDB3NDF; X-ray; 2.70 A; A=46-381
B=383-418PDB; 3T1P; X-ray; 3.90 A
A=48-418PDB; 7API; X-ray; 3.00 A
A=36-382B=383-418; PDB; 8API; X-ray
3.10 AA=36-382; B=383-418; PDB; 9API
X-ray3.00 A; A=36-382; B=383-418; PDBsum
1ATU-; .
PDBsum1D5S; -; .
PDBsum1EZX; -; .
PDBsum1HP7; -; .
PDBsum1IZ2; -; .
PDBsum1KCT; -; .
PDBsum1OO8; -; .
PDBsum1OPH; -; .
PDBsum1PSI; -; .
PDBsum1QLP; -; .
PDBsum1QMB; -; .
PDBsum2D26; -; .
PDBsum2QUG; -; .
PDBsum3CWL; -; .
PDBsum3CWM; -; .
PDBsum3DRM; -; .
PDBsum3DRU; -; .
PDBsum3NDD; -; .
PDBsum3NDF; -; .
PDBsum3T1P; -; .
PDBsum7API; -; .
PDBsum8API; -; .
PDBsum9API; -; .
ProteinModelPortalP01009; -; .
SMRP01009; 47-418; .
IntActP01009; 11; .
MINTMINT-365327; -; .
STRINGP01009; -; .
MEROPSI04.001; -; .
GlycoSuiteDBP01009; -; .
PhosphoSiteP01009; -; .
SWISS-2DPAGEP01009; -; .
Cornea-2DPAGEP01009; -; .
DOSAC-COBS-2DPAGEP01009; -; .
OGPP01009; -; .
REPRODUCTION-2DPAGEIPI00553177; -; .
REPRODUCTION-2DPAGEP01009; -; .
Siena-2DPAGEP01009; -; .
UCD-2DPAGEP01009; -; .
PRIDEP01009; -; .
EnsemblENST00000355814; ENSP00000348068; ENSG00000197249; .
EnsemblENST00000393087; ENSP00000376802; ENSG00000197249; .
EnsemblENST00000393088; ENSP00000376803; ENSG00000197249; .
EnsemblENST00000404814; ENSP00000385960; ENSG00000197249; .
EnsemblENST00000426226; ENSP00000415603; ENSG00000197249; .
EnsemblENST00000437074; ENSP00000408878; ENSG00000197249; .
EnsemblENST00000437397; ENSP00000408474; ENSG00000197249; .
EnsemblENST00000440909; ENSP00000390299; ENSG00000197249; .
EnsemblENST00000448140; ENSP00000416462; ENSG00000197249; .
EnsemblENST00000448921; ENSP00000416066; ENSG00000197249; .
EnsemblENST00000449399; ENSP00000416354; ENSG00000197249; .
GeneID5265; -; .
KEGGhsa:5265; -; .
UCSCuc001ycx.2; human; .
UCSCuc001yda.1; human; .
CTD5265; -; .
GeneCardsGC14M075022; -; .
H-InvDBHIX0011929; -; .
HGNCHGNC:8941; SERPINA1; .
HPACAB013211; -; .
HPACAB016648; -; .
HPAHPA000927; -; .
HPAHPA001292; -; .
MIM107400; gene; .
MIM613490; phenotype; .
neXtProtNX_P01009; -; .
Orphanet60; Alpha-1 antitrypsin deficiency; .
Orphanet178396; Hemorrhagic disease due to alpha-1 antitrypsin Pittsburgh mutation; .
PharmGKBPA35509; -; .
eggNOGprNOG09018; -; .
HOVERGENHBG005957; -; .
InParanoidP01009; -; .
OMAPMMNRLG; -; .
OrthoDBEOG4FR0S4; -; .
PhylomeDBP01009; -; .
Pathway_Interaction_DBhnf3apathway; FOXA1 transcription factor network; .
ReactomeREACT_604; Hemostasis; .
DrugBankDB00058; Alpha-1-proteinase inhibitor; .
NextBio20336; -; .
PMAP-CutDBP01009; -; .
ArrayExpressP01009; -; .
BgeeP01009; -; .
GenevestigatorP01009; -; .
GermOnlineENSG00000197249; Homo sapiens; .
GOGO:0005615; C:extracellular space; IMP:UniProtKB; .
GOGO:0031093; C:platelet alpha granule lumen; TAS:Reactome; .
GOGO:0005578; C:proteinaceous extracellular matrix; IEA:UniProtKB-SubCell; .
GOGO:0002020; F:protease binding; IPI:UniProtKB; .
GOGO:0004867; F:serine-type endopeptidase inhibitor activity; NAS:UniProtKB; .
GOGO:0006953; P:acute-phase response; IEA:UniProtKB-KW; .
GOGO:0030168; P:platelet activation; TAS:Reactome; .
GOGO:0002576; P:platelet degranulation; TAS:Reactome; .
GOGO:0030162; P:regulation of proteolysis; IBA:RefGenome; .
InterProIPR000215; Protease_inhib_I4_serpin; .
InterProIPR023795; Protease_inhib_I4_serpin_CS; .
InterProIPR023796; Sepin_dom; .
PANTHERPTHR11461; Prot_inh_serpin; 1; .
PfamPF00079; Serpin; 1; .
SMARTSM00093; SERPIN; 1; .
SUPFAMSSF56574; Prot_inh_serpin; 1; .
PROSITEPS00284; SERPIN; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server