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Searching in 'SWISS-2DPAGE' for entry matching: ACTB_HUMAN




SWISS-2DPAGE:  ACTB_HUMAN


ACTB_HUMAN


General information about the entry
View entry in simple text format
Entry nameACTB_HUMAN
Primary accession numberP60709
Secondary accession number(s) P02570
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 6)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed;.
Gene nameName=ACTB
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=79188222; PubMed=109131; [NCBI, ExPASy, EBI, Israel, Japan]
Edwards J., Anderson N.G., Nance S.L., Anderson N.L.
''''''Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins'';'';''
Blood 53(1):1121-1132(1979)
[4]   MAPPING ON GEL
MEDLINE=93120196; PubMed=1477117; [NCBI, ExPASy, EBI, Israel, Japan]
Golaz O., Walzer C., Hochstrasser D.F., Bjellqvist B., Turler H., Balant L.P.
''''''Red blood cell protein map: a comparison between carrier-ampholyte pH gradient and immobilized pH gradient, and identification of four red blood cell enzymes'';'';''
Appl. Theor. Electrophor. 3(1):77-82(1992)
[5]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''''''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis'';'';''
Electrophoresis 16(1):1152-1159(1995)
[6]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[7]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[8]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[9]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[10]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[11]   MAPPING ON GEL
PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
[12]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
Comments
  • SUBUNIT: SINGLE CHAIN WHICH CAN BIND UP TO 4 OTHER CHAINS
2D PAGE maps for identified proteins
How to interpret a protein

NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_1D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_1D_HUMAN

MAP LOCATIONS:
Mw=73806  [identification data]

MAPPING (identification):
Tandem mass spectrometry [11].



CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=5.05; Mw=41700
pI=5.11; Mw=41785

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [9].



CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=5.23; Mw=44803

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=5.15; Mw=41700  [identification data]
pI=5.18; Mw=41700  [identification data]
pI=5.09; Mw=41539  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [10].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=5.22; Mw=41432
pI=5.13; Mw=41564

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=5.15; Mw=41700
pI=5.09; Mw=41700
pI=5.23; Mw=41233

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=5.28; Mw=38400
pI=5.16; Mw=38311
pI=5.22; Mw=38135
pI=5.12; Mw=38135

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=5.26; Mw=41605
pI=5.16; Mw=41070
pI=5.08; Mw=41177

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [7][8].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=5.19; Mw=41311
pI=5.26; Mw=41600
pI=5.14; Mw=41503
pI=5.12; Mw=41407

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL AND IMMUNODETECTION [6].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.26; Mw=41836
pI=5.19; Mw=41836
pI=5.22; Mw=41605

MAPPING (identification):
MATCHING WITH A PLASMA GEL [1].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=5.29; Mw=41600

MAPPING (identification):
GEL MATCHING WITH THE LYMPHOMA MASTER GEL [12].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=5.26; Mw=41800
pI=5.16; Mw=42093

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



NUCLEOLI_HELA_2D_HUMAN {2D-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_2D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_2D_HUMAN

MAP LOCATIONS:
pI=5.47; Mw=44313  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [11].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=5.28; Mw=43509
pI=5.24; Mw=43156

NORMAL LEVEL:
PLATELET CONTAMINATION.

MAPPING (identification):
IMMUNOBLOTTING [3].



PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=5.26; Mw=41400
pI=5.16; Mw=41061
pI=5.05; Mw=41061
pI=5.30; Mw=41061

MAPPING (identification):
MATCHING WITH RBC AND LIVER MASTERS [5].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=5.20; Mw=42000
pI=5.14; Mw=42106
pI=5.27; Mw=42000

MAPPING (identification):
IMMUNOBLOTTING [3] AND MATCHING [4].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=5.22; Mw=41605

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP60709; P60709.
DOSAC-COBS-2DPAGEP60709; ACTB_HUMAN.
Lens-2DPAGEP02570.
OGP-WWWP02570.
REPRODUCTION-2DPAGEP60709; P60709.
Siena-2DPAGEP02570; ACTB_HUMAN.
UCD-2DPAGEP60709; ACTB_HUMAN.
UniProtKB/Swiss-ProtP60709; ACTB_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameACTB_HUMAN
Primary accession numberP60709
Secondary accession number(s) P02570 P70514 P99021 Q11211 Q64316 Q75MN2 Q96B34 Q96HG5
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on October 19, 2011 (version 99)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed;
Gene nameName=ACTB
Encoded onName=ACTB
Keywords3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Deafness; Direct protein sequencing; Disease mutation; Dystonia; Methylation; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX00351; CAA25099.1; -; mRNA
EMBLM10277; AAA51567.1; -; Genomic_DNA
EMBLX63432; CAA45026.1; -; mRNA
EMBLAY582799; AAS79319.1; -; Genomic_DNA
EMBLAC006483; AAP22343.1; -; Genomic_DNA
EMBLBC001301; AAH01301.1; -; mRNA
EMBLBC002409; AAH02409.1; -; mRNA
EMBLBC004251; AAH04251.1; -; mRNA
EMBLBC008633; AAH08633.1; -; mRNA
EMBLBC012854; AAH12854.1; -; mRNA
EMBLBC013380; AAH13380.1; -; mRNA
EMBLBC014861; AAH14861.1; -; mRNA
EMBLBC016045; AAH16045.1; -; mRNA
EMBLV00478; CAA23745.1; -; mRNA
IPIIPI00021439; -; .
PIRA25168; ATHUB; .
RefSeqNP_001092.1; NM_001101.3; .
UniGeneHs.520640; -; .
UniGeneHs.708120; -; .
UniGeneHs.727576; -; .
PDB3BYH; EM; 12.00 A; A=2-375
PDB3D2U; X-ray; 2.21 A; C/G=170-178
PDB3LUE; EM; -; A/B/C/D/E/F/G/H/I/J=2-375
PDBsum3BYH; -; .
PDBsum3D2U; -; .
PDBsum3LUE; -; .
ProteinModelPortalP60709; -; .
SMRP60709; 2-375; .
DIPDIP-29686N; -; .
IntActP60709; 115; .
MINTMINT-220312; -; .
STRINGP60709; -; .
PhosphoSiteP60709; -; .
SWISS-2DPAGEP60709; -; .
Aarhus/Ghent-2DPAGE7316; IEF; .
DOSAC-COBS-2DPAGEP60709; -; .
DOSAC-COBS-2DPAGEP60709_OR_P63261; -; .
REPRODUCTION-2DPAGEP60709; -; .
Siena-2DPAGEP60709; -; .
UCD-2DPAGEP60709; -; .
PeptideAtlasP60709; -; .
PRIDEP60709; -; .
EnsemblENST00000331789; ENSP00000349960; ENSG00000075624; .
GeneID60; -; .
KEGGhsa:60; -; .
UCSCuc003sos.2; human; .
CTD60; -; .
GeneCardsGC07M005479; -; .
H-InvDBHIX0006454; -; .
HGNCHGNC:132; ACTB; .
HPACAB002621; -; .
MIM102630; gene; .
MIM607371; phenotype; .
neXtProtNX_P60709; -; .
Orphanet79107; Developmental malformations - deafness - dystonia; .
PharmGKBPA24457; -; .
eggNOGprNOG18221; -; .
HOVERGENHBG003771; -; .
InParanoidP60709; -; .
OMAIKNLMER; -; .
OrthoDBEOG41JZC9; -; .
PhylomeDBP60709; -; .
ReactomeREACT_11123; Membrane Trafficking; .
ReactomeREACT_17015; Metabolism of proteins; .
ReactomeREACT_18266; Axon guidance; .
ReactomeREACT_20676; Cell junction organization; .
ReactomeREACT_604; Hemostasis; .
NextBio253; -; .
ArrayExpressP60709; -; .
BgeeP60709; -; .
CleanExHS_ACTB; -; .
GenevestigatorP60709; -; .
GermOnlineENSG00000075624; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0070688; C:MLL5-L complex; IDA:UniProtKB; .
GOGO:0035267; C:NuA4 histone acetyltransferase complex; IDA:UniProtKB; .
GOGO:0030529; C:ribonucleoprotein complex; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0019894; F:kinesin binding; IPI:UniProtKB; .
GOGO:0050998; F:nitric-oxide synthase binding; IPI:BHF-UCL; .
GOGO:0005200; F:structural constituent of cytoskeleton; TAS:UniProtKB; .
GOGO:0030957; F:Tat protein binding; IPI:BHF-UCL; .
GOGO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome; .
GOGO:0034332; P:adherens junction organization; TAS:Reactome; .
GOGO:0007411; P:axon guidance; TAS:Reactome; .
GOGO:0007596; P:blood coagulation; TAS:Reactome; .
GOGO:0034329; P:cell junction assembly; TAS:Reactome; .
GOGO:0006928; P:cellular component movement; TAS:UniProtKB; .
InterProIPR004000; Actin-like; .
InterProIPR020902; Actin/actin-like_CS; .
InterProIPR004001; Actin_CS; .
PANTHERPTHR11937; Actin_like; 1; .
PfamPF00022; Actin; 1; .
PRINTSPR00190; ACTIN; .
SMARTSM00268; ACTIN; 1; .
PROSITEPS00406; ACTINS_1; 1; .
PROSITEPS00432; ACTINS_2; 1; .
PROSITEPS01132; ACTINS_ACT_LIKE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server