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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: C1TC_HUMAN




SWISS-2DPAGE:  C1TC_HUMAN


C1TC_HUMAN


General information about the entry
View entry in simple text format
Entry nameC1TC_HUMAN
Primary accession numberP11586
integrated into SWISS-2DPAGE on January 15, 1999 (release 9)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=C-1-tetrahydrofolate synthase, cytoplasmic; Short=C1-THF synthase; Includes: RecName: Full=Methylenetetrahydrofolate dehydrogenase; EC=1.5.1.5; Includes: RecName: Full=Methenyltetrahydrofolate cyclohydrolase; EC=3.5.4.9; Includes: RecName: Full=Formyltetrahydrofolate synthetase; EC=6.3.4.3;.
Gene nameName=MTHFD1
Synonyms=MTHFC, MTHFD
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=6.79; Mw=125156  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
REPRODUCTION-2DPAGEIPI00218342; IPI00218342.
UniProtKB/Swiss-ProtP11586; C1TC_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameC1TC_HUMAN
Primary accession numberP11586
Secondary accession number(s) B2R5Y2 Q86VC9 Q9BVP5
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 140)
Name and origin of the protein
DescriptionRecName: Full=C-1-tetrahydrofolate synthase, cytoplasmic; Short=C1-THF synthase; Includes: RecName: Full=Methylenetetrahydrofolate dehydrogenase; EC=1.5.1.5; Includes: RecName: Full=Methenyltetrahydrofolate cyclohydrolase; EC=3.5.4.9; Includes: RecName: Full=Formyltetrahydrofolate synthetase; EC=6.3.4.3;
Gene nameName=MTHFD1
Synonyms=MTHFC, MTHFD
Encoded onName=MTHFD1; Synonyms=MTHFC, MTHFD
Keywords3D-structure; Acetylation; Amino-acid biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Histidine biosynthesis; Hydrolase; Ligase; Methionine biosynthesis; Multifunctional enzyme; NADP; Nucleotide-binding; One-carbon metabolism; Oxidoreductase; Phosphoprotein; Polymorphism; Purine biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ04031; AAA59574.1; -; mRNA
EMBLAK312361; BAG35279.1; -; mRNA
EMBLCH471061; EAW80857.1; -; Genomic_DNA
EMBLBC001014; AAH01014.2; -; mRNA
EMBLBC009806; AAH09806.1; -; mRNA
EMBLBC050420; AAH50420.1; -; mRNA
IPIIPI00218342; -; .
PIRA31903; A31903; .
RefSeqNP_005947.3; NM_005956.3; .
UniGeneHs.652308; -; .
PDB1A4I; X-ray; 1.50 A; A/B=1-301
PDB1DIA; X-ray; 2.20 A; A/B=1-306
PDB1DIB; X-ray; 2.70 A; A/B=1-306
PDB1DIG; X-ray; 2.20 A; A/B=1-306
PDBsum1A4I; -; .
PDBsum1DIA; -; .
PDBsum1DIB; -; .
PDBsum1DIG; -; .
ProteinModelPortalP11586; -; .
SMRP11586; 2-296; 319-933; .
IntActP11586; 6; .
MINTMINT-5000922; -; .
STRINGP11586; -; .
PhosphoSiteP11586; -; .
SWISS-2DPAGEP11586; -; .
REPRODUCTION-2DPAGEIPI00218342; -; .
PRIDEP11586; -; .
EnsemblENST00000216605; ENSP00000216605; ENSG00000100714; .
GeneID4522; -; .
KEGGhsa:4522; -; .
UCSCuc001xhb.1; human; .
CTD4522; -; .
GeneCardsGC14P045027; -; .
H-InvDBHIX0011731; -; .
HGNCHGNC:7432; MTHFD1; .
HPAHPA000704; -; .
HPAHPA001290; -; .
HPAHPA015006; -; .
MIM114500; phenotype; .
MIM172460; gene+phenotype; .
MIM601634; phenotype; .
neXtProtNX_P11586; -; .
Orphanet3388; Neural tube defect; .
PharmGKBPA31236; -; .
eggNOGprNOG05153; -; .
GeneTreeENSGT00600000084426; -; .
HOVERGENHBG004916; -; .
InParanoidP11586; -; .
OrthoDBEOG4GB75D; -; .
PhylomeDBP11586; -; .
BioCycMetaCyc:MONOMER-11654; -; .
BRENDA6.3.4.3; 2681; .
ReactomeREACT_11193; Metabolism of vitamins and cofactors; .
DrugBankDB00157; NADH; .
DrugBankDB00116; Tetrahydrofolic acid; .
NextBio17468; -; .
ArrayExpressP11586; -; .
BgeeP11586; -; .
CleanExHS_MTHFD1; -; .
GenevestigatorP11586; -; .
GermOnlineENSG00000100714; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005739; C:mitochondrion; TAS:ProtInc; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004329; F:formate-tetrahydrofolate ligase activity; TAS:Reactome; .
GOGO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; TAS:Reactome; .
GOGO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:EC; .
GOGO:0004486; F:methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity; TAS:Reactome; .
GOGO:0046655; P:folic acid metabolic process; TAS:Reactome; .
GOGO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro; .
GOGO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW; .
GOGO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW; .
InterProIPR000559; Formate_THF_ligase; .
InterProIPR020628; Formate_THF_ligase_CS; .
InterProIPR016040; NAD(P)-bd_dom; .
InterProIPR000672; THF_DH/CycHdrlase; .
InterProIPR020630; THF_DH/CycHdrlase_cat_dom; .
InterProIPR020867; THF_DH/CycHdrlase_CS; .
InterProIPR020631; THF_DH/CycHdrlase_NAD-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PfamPF01268; FTHFS; 1; .
PfamPF00763; THF_DHG_CYH; 1; .
PfamPF02882; THF_DHG_CYH_C; 1; .
PRINTSPR00085; THFDHDRGNASE; .
PROSITEPS00721; FTHFS_1; 1; .
PROSITEPS00722; FTHFS_2; 1; .
PROSITEPS00766; THF_DHG_CYH_1; 1; .
PROSITEPS00767; THF_DHG_CYH_2; 1; .



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