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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: CAH3_MOUSE




SWISS-2DPAGE:  CAH3_MOUSE


CAH3_MOUSE


General information about the entry
View entry in simple text format
Entry nameCAH3_MOUSE
Primary accession numberP16015
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onOctober 1, 2001 (version 5)
General Annotations were last modified on May 19, 2011 (version 8)
Name and origin of the protein
DescriptionRecName: Full=Carbonic anhydrase 3; EC=4.2.1.1; AltName: Full=Carbonate dehydratase III; AltName: Full=Carbonic anhydrase III; Short=CA-III;.
Gene nameName=Ca3
Synonyms=Car3
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96154592; PubMed=8586073; [NCBI, Expasy, EBI, Israel, Japan]
Anderson N.L., Esquer-Blasco R., Hoffmann J.-P., Meheus L., Raymackers J., Steiner S., Witzmann F., Anderson N.G.
''''''An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies'';'';''
Electrophoresis 16(1):1977-1981(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, Expasy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
[3]   MAPPING ON GEL
PubMed=11503206; [NCBI, Expasy, EBI, Israel, Japan]
Lanne B., Potthast F., Hoglund A., Brockenhuus Von Lowenhielm H., Nystrom A.-C., Nilsson F., Dahllof B.
''''''Thiourea enhances mapping of the proteome from murine white adipose tissue'';'';''
Proteomics 1(1):819-828(2001)
[4]   MAPPING ON GEL
Tonella L., Hoogland C., Sanchez J.-C.
Submitted (OCT-2001) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

BAT_MOUSE {Brown adipose tissue}
Mus musculus (Mouse)
Tissue: Brown adipose tissue
BAT_MOUSE
  map experimental info
  protein estimated location
 
BAT_MOUSE

MAP LOCATIONS:
pI=7.01; Mw=30184
pI=6.86; Mw=29526

MAPPING (identification):
GEL MATCHING WITH WAT_MOUSE [2].



LIVER_MOUSE {Liver}
Mus musculus (Mouse)
Tissue: Liver
LIVER_MOUSE
  map experimental info
  protein estimated location
 
LIVER_MOUSE

MAP LOCATIONS:
pI=6.98; Mw=28122
pI=6.84; Mw=27831
pI=6.74; Mw=27889

MAPPING (identification):
MATCHING [2] WITH ANDERSON MOUSE AND RAT LIVER 2-DE MAPS [1].



MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=6.88; Mw=27220  [identification data]
pI=6.79; Mw=27163  [identification data]

MAPPING (identification):
MATCHING [2] WITH THE MOUSE LIVER MASTER GEL [2], AND MASS FINGERPRINTING [2].



WAT_MOUSE {White adipose tissue}
Mus musculus (Mouse)
Tissue: White adipose tissue
WAT_MOUSE
  map experimental info
  protein estimated location
 
WAT_MOUSE

MAP LOCATIONS:
pI=6.97; Mw=32082
pI=6.83; Mw=31514
pI=6.70; Mw=31374
pI=7.75; Mw=14353

MAPPING (identification):
SPOTS 1B*: MATCHING WITH THE MOUSE LIVER MASTER GEL [2];
SPOT 2D-001CD8: GEL MATCHING [3] WITH WAT_MOUSE 2-DE MAP FROM LANNE ET AL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
UniProtKB/Swiss-ProtP16015; CAH3_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCAH3_MOUSE
Primary accession numberP16015
Secondary accession number(s) Q9ERN8
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on September 21, 2011 (version 97)
Name and origin of the protein
DescriptionRecName: Full=Carbonic anhydrase 3; EC=4.2.1.1; AltName: Full=Carbonate dehydratase III; AltName: Full=Carbonic anhydrase III; Short=CA-III;
Gene nameName=Ca3
Synonyms=Car3
Encoded onName=Ca3; Synonyms=Car3
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; Glutathionylation; Lyase; Metal-binding; Reference proteome; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM27796; AAA37355.1; -; mRNA
EMBLAF294988; AAG22029.1; -; Genomic_DNA
EMBLAF294983; AAG22029.1; JOINED; Genomic_DNA
EMBLAF294984; AAG22029.1; JOINED; Genomic_DNA
EMBLAF294985; AAG22029.1; JOINED; Genomic_DNA
EMBLAF294986; AAG22029.1; JOINED; Genomic_DNA
EMBLAF294987; AAG22029.1; JOINED; Genomic_DNA
EMBLBC011129; AAH11129.1; -; mRNA
IPIIPI00221890; -; .
PIRA43641; A43641; .
RefSeqNP_031632.2; NM_007606.3; .
UniGeneMm.300; -; .
ProteinModelPortalP16015; -; .
SMRP16015; 2-260; .
IntActP16015; 1; .
STRINGP16015; -; .
PhosphoSiteP16015; -; .
SWISS-2DPAGEP16015; -; .
PRIDEP16015; -; .
EnsemblENSMUST00000029076; ENSMUSP00000029076; ENSMUSG00000027559; .
GeneID12350; -; .
KEGGmmu:12350; -; .
UCSCuc008oqs.1; mouse; .
CTD12350; -; .
MGIMGI:88270; Car3; .
eggNOGroNOG15075; -; .
GeneTreeENSGT00560000076828; -; .
HOGENOMHBG717384; -; .
HOVERGENHBG002837; -; .
InParanoidP16015; -; .
OMALQPWSVS; -; .
OrthoDBEOG4001JV; -; .
PhylomeDBP16015; -; .
NextBio281000; -; .
ArrayExpressP16015; -; .
BgeeP16015; -; .
CleanExMM_CAR3; -; .
GenevestigatorP16015; -; .
GermOnlineENSMUSG00000027559; Mus musculus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0004089; F:carbonate dehydratase activity; IEA:EC; .
GOGO:0016151; F:nickel ion binding; IDA:MGI; .
GOGO:0008270; F:zinc ion binding; IEA:InterPro; .
GOGO:0006730; P:one-carbon metabolic process; IEA:InterPro; .
InterProIPR023561; Carbonic_anhydrase_a-class; .
InterProIPR001148; Carbonic_anhydrase_a-class_cat; .
InterProIPR018338; Carbonic_anhydrase_a-class_CS; .
InterProIPR018441; Carbonic_anhydrase_CA3; .
Gene3DG3DSA:3.10.200.10; Euk_COanhd; 1; .
PANTHERPTHR18952:SF29; Carbonic_anhydrase_CA3; 1; .
PANTHERPTHR18952; Euk_COanhd; 1; .
PfamPF00194; Carb_anhydrase; 1; .
SUPFAMSSF51069; Euk_COanhd; 1; .
PROSITEPS00162; ALPHA_CA_1; 1; .
PROSITEPS51144; ALPHA_CA_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server