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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ECHM_HUMAN




SWISS-2DPAGE:  ECHM_HUMAN


ECHM_HUMAN


General information about the entry
View entry in simple text format
Entry nameECHM_HUMAN
Primary accession numberP30084
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onDecember 1, 2000 (version 2)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Enoyl-CoA hydratase, mitochondrial; EC=4.2.1.17; AltName: Full=Enoyl-CoA hydratase 1; AltName: Full=Short-chain enoyl-CoA hydratase; Short=SCEH; Flags: Precursor;.
Gene nameName=ECHS1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=94147970; PubMed=8313871; [NCBI, ExPASy, EBI, Israel, Japan]
Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C., Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P., Hochstrasser D.F.
''''''Plasma and red blood cell protein maps: update 1993'';'';''
Electrophoresis 14(1):1223-1231(1993)
[3]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[4]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
Comments
  • SUBUNIT: HOMOHEXAMER
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=5.95; Mw=25821

MAPPING (identification):
MICROSEQUENCING [4].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=6.21; Mw=24544

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.92; Mw=26179
pI=5.57; Mw=26120

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=5.91; Mw=26093

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=5.93; Mw=26000

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=5.86; Mw=26133

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP30084; P30084.
DOSAC-COBS-2DPAGEP30084; ECHM_HUMAN.
REPRODUCTION-2DPAGEP30084; P30084.
REPRODUCTION-2DPAGEIPI00024993; IPI00024993.
Siena-2DPAGEP30084; ECHM_HUMAN.
UCD-2DPAGEP30084; ECHM_HUMAN.
UniProtKB/Swiss-ProtP30084; ECHM_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameECHM_HUMAN
Primary accession numberP30084
Secondary accession number(s) O00739 Q5VWY1 Q96H54
Sequence was last modified on April 26, 2005 (version 4)
Annotations were last modified on October 19, 2011 (version 127)
Name and origin of the protein
DescriptionRecName: Full=Enoyl-CoA hydratase, mitochondrial; EC=4.2.1.17; AltName: Full=Enoyl-CoA hydratase 1; AltName: Full=Short-chain enoyl-CoA hydratase; Short=SCEH; Flags: Precursor;
Gene nameName=ECHS1
Encoded onName=ECHS1
Keywords3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Lipid metabolism; Lyase; Mitochondrion; Polymorphism; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD13900; BAA03001.1; -; mRNA
EMBLX98126; CAA66808.1; -; Genomic_DNA
EMBLX98127; CAA66808.1; JOINED; Genomic_DNA
EMBLX98128; CAA66808.1; JOINED; Genomic_DNA
EMBLX98129; CAA66808.1; JOINED; Genomic_DNA
EMBLBT007123; AAP35787.1; -; mRNA
EMBLAL360181; CAH70286.1; -; Genomic_DNA
EMBLBC008906; AAH08906.1; -; mRNA
IPIIPI00024993; -; .
RefSeqNP_004083.3; NM_004092.3; .
UniGeneHs.76394; -; .
PDB2HW5; X-ray; 2.55 A; A/B/C/D/E/F=28-290
PDBsum2HW5; -; .
ProteinModelPortalP30084; -; .
SMRP30084; 31-290; .
IntActP30084; 6; .
MINTMINT-1401929; -; .
STRINGP30084; -; .
PhosphoSiteP30084; -; .
SWISS-2DPAGEP30084; -; .
DOSAC-COBS-2DPAGEP30084; -; .
REPRODUCTION-2DPAGEIPI00024993; -; .
REPRODUCTION-2DPAGEP30084; -; .
Siena-2DPAGEP30084; -; .
UCD-2DPAGEP30084; -; .
PeptideAtlasP30084; -; .
PRIDEP30084; -; .
EnsemblENST00000368547; ENSP00000357535; ENSG00000127884; .
GeneID1892; -; .
KEGGhsa:1892; -; .
NMPDRfig|9606.3.peg.4902; -; .
UCSCuc001lmu.1; human; .
CTD1892; -; .
GeneCardsGC10M128720; -; .
H-InvDBHIX0009333; -; .
HGNCHGNC:3151; ECHS1; .
HPACAB003783; -; .
HPAHPA021995; -; .
HPAHPA022476; -; .
MIM602292; gene; .
neXtProtNX_P30084; -; .
PharmGKBPA27597; -; .
eggNOGprNOG11719; -; .
GeneTreeENSGT00580000081296; -; .
HOGENOMHBG748731; -; .
HOVERGENHBG010157; -; .
InParanoidP30084; -; .
OMAYETTLTE; -; .
OrthoDBEOG4P2Q32; -; .
PhylomeDBP30084; -; .
ReactomeREACT_22258; Metabolism of lipids and lipoproteins; .
ArrayExpressP30084; -; .
BgeeP30084; -; .
CleanExHS_ECHS1; -; .
GenevestigatorP30084; -; .
GermOnlineENSG00000127884; Homo sapiens; .
GOGO:0005759; C:mitochondrial matrix; TAS:Reactome; .
GOGO:0004300; F:enoyl-CoA hydratase activity; TAS:Reactome; .
GOGO:0005515; F:protein binding; IPI:HGNC; .
GOGO:0006635; P:fatty acid beta-oxidation; TAS:Reactome; .
InterProIPR014748; Crontonase_C; .
InterProIPR001753; Crotonase_core; .
InterProIPR018376; Enoyl-CoA_hyd/isom_CS; .
Gene3DG3DSA:1.10.12.10; Crontonase_C; 1; .
PfamPF00378; ECH; 1; .
PROSITEPS00166; ENOYL_COA_HYDRATASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server