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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: EF1B_HUMAN




SWISS-2DPAGE:  EF1B_HUMAN


EF1B_HUMAN


General information about the entry
View entry in simple text format
Entry nameEF1B_HUMAN
Primary accession numberP24534
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMay 15, 2003 (version 4)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 1-beta; Short=EF-1-beta;.
Gene nameName=EEF1B2
Synonyms=EEF1B, EF1B
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[4]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[5]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
Comments
  • SUBUNIT: ALPHA, BETA, DELTA, AND GAMMA
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=4.54; Mw=30421

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=4.50; Mw=28086

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [5].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=4.55; Mw=28063

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=4.51; Mw=28602

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=4.57; Mw=28635

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.53; Mw=28206

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=4.54; Mw=28196

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=4.47; Mw=29497

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP24534; P24534.
DOSAC-COBS-2DPAGEP24534; EF1B_HUMAN.
OGP-WWWP24534.
PHCI-2DPAGEP24534; EF1B_HUMAN.
Siena-2DPAGEP24534; EF1B_HUMAN.
UniProtKB/Swiss-ProtP24534; EF1B_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameEF1B_HUMAN
Primary accession numberP24534
Secondary accession number(s) A8K795 Q6IBH9
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 138)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor 1-beta; Short=EF-1-beta;
Gene nameName=EEF1B2
Synonyms=EEF1B, EF1B
Encoded onName=EEF1B2; Synonyms=EEF1B, EF1B
Keywords3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Elongation factor; Phosphoprotein; Protein biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX60489; CAA43019.1; -; mRNA
EMBLX60656; CAA43063.1; -; mRNA
EMBLBT007079; AAP35742.1; -; mRNA
EMBLCR456825; CAG33106.1; -; mRNA
EMBLAK291910; BAF84599.1; -; mRNA
EMBLAC007383; AAY15062.1; -; Genomic_DNA
EMBLCH471063; EAW70381.1; -; Genomic_DNA
EMBLBC000211; AAH00211.1; -; mRNA
EMBLBC004931; AAH04931.1; -; mRNA
EMBLBC067787; AAH67787.1; -; mRNA
IPIIPI00178440; -; .
PIRS25432; S25432; .
RefSeqNP_001032752.1; NM_001037663.1; .
RefSeqNP_001950.1; NM_001959.3; .
RefSeqNP_066944.1; NM_021121.3; .
UniGeneHs.421608; -; .
PDB1B64; NMR; -; A=136-224
PDBsum1B64; -; .
ProteinModelPortalP24534; -; .
SMRP24534; 10-72; 136-225; .
IntActP24534; 17; .
MINTMINT-4999956; -; .
STRINGP24534; -; .
PhosphoSiteP24534; -; .
SWISS-2DPAGEP24534; -; .
Aarhus/Ghent-2DPAGE9206; IEF; .
Aarhus/Ghent-2DPAGE9207; IEF; .
Aarhus/Ghent-2DPAGE9209; IEF; .
DOSAC-COBS-2DPAGEP24534; -; .
OGPP24534; -; .
PHCI-2DPAGEP24534; -; .
Siena-2DPAGEP24534; -; .
PeptideAtlasP24534; -; .
PRIDEP24534; -; .
EnsemblENST00000236957; ENSP00000236957; ENSG00000114942; .
EnsemblENST00000392221; ENSP00000376055; ENSG00000114942; .
EnsemblENST00000392222; ENSP00000376056; ENSG00000114942; .
GeneID1933; -; .
KEGGhsa:1933; -; .
UCSCuc002vbf.1; human; .
CTD1933; -; .
GeneCardsGC02P207024; -; .
GeneCardsGC02P207027; -; .
H-InvDBHIX0002770; -; .
HGNCHGNC:3208; EEF1B2; .
HPACAB012477; -; .
MIM600655; gene; .
neXtProtNX_P24534; -; .
PharmGKBPA27644; -; .
GeneTreeENSGT00390000011747; -; .
HOGENOMHBG714747; -; .
HOVERGENHBG000787; -; .
InParanoidP24534; -; .
OMAFSRWFNH; -; .
OrthoDBEOG4Z8XXH; -; .
PhylomeDBP24534; -; .
ReactomeREACT_17015; Metabolism of proteins; .
ReactomeREACT_71; Gene Expression; .
NextBio7829; -; .
PMAP-CutDBP24534; -; .
BgeeP24534; -; .
CleanExHS_EEF1B2; -; .
GenevestigatorP24534; -; .
GermOnlineENSG00000114942; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005853; C:eukaryotic translation elongation factor 1 complex; NAS:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0003746; F:translation elongation factor activity; NAS:UniProtKB; .
InterProIPR018940; EF-1_beta_acid_region_euk; .
InterProIPR010987; Glutathione-S-Trfase_C-like; .
InterProIPR014717; Transl_elong_EF1B/ribosomal_S6; .
InterProIPR001326; Transl_elong_EF1B_B/D_CS; .
InterProIPR014038; Transl_elong_fac_EF1B_bsu/dsu; .
Gene3DG3DSA:1.20.1050.10; GST_C_like; 1; .
Gene3DG3DSA:3.30.70.60; Transl_elong_EF1B/rib_con; 1; .
PfamPF10587; EF-1_beta_acid; 1; .
PfamPF00736; EF1_GNE; 1; .
SMARTSM00888; EF1_GNE; 1; .
SUPFAMSSF47616; GST_C_like; 1; .
SUPFAMSSF54984; Transl_elong_EF1B_B/D_G_exch; 1; .
PROSITEPS00824; EF1BD_1; 1; .
PROSITEPS00825; EF1BD_2; 1; .
PROSITEPS50405; GST_CTER; FALSE_NEG; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server