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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: HPT_HUMAN




SWISS-2DPAGE:  HPT_HUMAN


HPT_HUMAN


General information about the entry
View entry in simple text format
Entry nameHPT_HUMAN
Primary accession numberP00738
Secondary accession number(s) P00737
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 4)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=Haptoglobin; Contains: RecName: Full=Haptoglobin alpha chain; Contains: RecName: Full=Haptoglobin beta chain; Flags: Precursor;.
Gene nameName=HP
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   GENETIC VARIANTS
MEDLINE=83019292; PubMed=6751044; [NCBI, ExPASy, EBI, Israel, Japan]
Bowman B.H., Kurosky A.
''''''Haptoglobin: the evolutionary product of duplication, unequal crossing over, and point mutation'';'';''
Adv. Hum. Genet. 12(1):189-261(1982)
[3]   MAPPING ON GEL
MEDLINE=78094420; PubMed=271964; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Anderson N.G.
''''''High resolution two-dimensional electrophoresis of human plasma proteins'';'';''
Proc. Natl. Acad. Sci. U.S.A. 74(1):5421-5425(1977)
[4]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[5]   MAPPING ON GEL
MEDLINE=93092937; PubMed=1459097; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.-C., James R.W., Tissot J.-D., Bjellqvist B., Hochstrasser D.F.
''''''Plasma protein map: an update by microsequencing'';'';''
Electrophoresis 13(1):707-714(1992)
[6]   MAPPING ON GEL
MEDLINE=97397181; PubMed=9253242; [NCBI, ExPASy, EBI, Israel, Japan]
Eckerskorn C., Strupat K., Schleuder D., Hochstrasser D.F., Sanchez J.-C., Lottspeich F., Hillenkamp F.
''''''Analysis of proteins by direct-scanning infrared-MALDI mass spectrometry after 2D-PAGE separation and electroblotting'';'';''
Anal. Chem. 69(1):2888-2892(1997)
Comments
  • SUBUNIT: TETRAMER OF TWO ALPHA-1 AND TWO BETA CHAINS
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=4.68; Mw=47026
pI=4.80; Mw=45624
pI=4.69; Mw=45486
pI=4.92; Mw=44398
pI=4.81; Mw=43864
pI=5.07; Mw=43600
pI=5.20; Mw=42428
pI=5.36; Mw=42557
pI=5.46; Mw=42428
pI=4.93; Mw=42557
pI=5.07; Mw=42428
pI=5.36; Mw=40915
pI=5.43; Mw=41288
pI=5.20; Mw=40915
pI=5.52; Mw=40112
pI=5.44; Mw=40212
pI=5.40; Mw=17109
pI=5.67; Mw=17109
pI=5.42; Mw=9374
pI=5.71; Mw=9270

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [4].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=4.71; Mw=41812
pI=4.99; Mw=40598
pI=5.33; Mw=39290
pI=5.44; Mw=39162
pI=5.58; Mw=38400
pI=5.61; Mw=15926

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [4].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.94; Mw=40767
pI=5.05; Mw=40584
pI=5.05; Mw=40037
pI=5.19; Mw=39856
pI=5.19; Mw=39320
pI=5.30; Mw=39054
pI=6.30; Mw=15953
pI=6.72; Mw=15804
pI=5.19; Mw=12883

NORMAL POSITIONAL VARIANTS:
HP1-1; HP2-1; HP2-2; SOME RARE VARIANTS [2].

PATHOLOGICAL LEVEL:
DECREASED DURING LIVER DISEASE AND HAEMOLYTIC ANAEMIAS; INCREASED DURING ACUTE-PHASE.

MAPPING (identification):
MATCHING WITH A PLASMA GEL [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=4.90; Mw=41625
pI=4.90; Mw=40848
pI=5.05; Mw=40592
pI=5.06; Mw=39918
pI=5.21; Mw=39751
pI=5.22; Mw=39009
pI=5.34; Mw=38683

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=4.88; Mw=44344
pI=4.81; Mw=44464
pI=4.97; Mw=43391
pI=4.88; Mw=43156
pI=5.21; Mw=42229
pI=5.08; Mw=42114
pI=4.97; Mw=42114
pI=5.37; Mw=42000
pI=5.08; Mw=41412
pI=5.21; Mw=41329
pI=5.36; Mw=40832
pI=5.21; Mw=40668
pI=5.69; Mw=40342
pI=5.55; Mw=40423
pI=5.21; Mw=39141
pI=5.38; Mw=38749
pI=5.56; Mw=38516
pI=5.69; Mw=38206
pI=5.86; Mw=37294
pI=5.68; Mw=17069
pI=5.40; Mw=16882
pI=6.07; Mw=16882
pI=5.37; Mw=11990
pI=5.13; Mw=11861

NORMAL LEVEL:
1000-3000 MG/L.

PATHOLOGICAL LEVEL:
DECREASED DURING LIVER DISEASE AND HAEMOLYTIC ANAEMIAS; INCREASED DURING ACUTE-PHASE.

POSITIONAL VARIANTS:
HP1-1; HP2-1; HP2-2; SOME RARE VARIANTS [2].

MAPPING (identification):
SPOTS 2D-00*: IMMUNOBLOTTING [3]; SPOTS 2D-0005G*: MICROSEQUENCING [5] AND MASS spectrometry [6].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP00738; P00738.
DOSAC-COBS-2DPAGEP00738; HPT_HUMAN.
HSC-2DPAGEP00737; HUMAN.
Siena-2DPAGEP00738; HPT_HUMAN.
UCD-2DPAGEP00737; HPT_HUMAN.
UniProtKB/Swiss-ProtP00738; HPT_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameHPT_HUMAN
Primary accession numberP00738
Secondary accession number(s) B0AZL5 P00737 Q0VAC4 Q2PP15 Q3B7J0 Q6LBY9 Q9UC67
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on September 21, 2011 (version 132)
Name and origin of the protein
DescriptionRecName: Full=Haptoglobin; Contains: RecName: Full=Haptoglobin alpha chain; Contains: RecName: Full=Haptoglobin beta chain; Flags: Precursor;
Gene nameName=HP
Encoded onName=HP
KeywordsComplete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Hemoglobin-binding; Polymorphism; Reference proteome; Repeat; Secreted; Serine protease homolog; Signal; Sushi.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLK00422; AAA52687.1; -; mRNA
EMBLK01763; AAA52684.1; -; mRNA
EMBLL29394; AAA52685.1; -; mRNA
EMBLX00637; CAA25267.1; -; mRNA
EMBLX01793; CAA25926.1; -; Genomic_DNA
EMBLX01786; CAA25926.1; JOINED; Genomic_DNA
EMBLX02206; CAA25926.1; JOINED; Genomic_DNA
EMBLX01789; CAA25926.1; JOINED; Genomic_DNA
EMBLX01791; CAA25926.1; JOINED; Genomic_DNA
EMBLM10935; AAA88080.1; -; Genomic_DNA
EMBLM69197; AAA88078.1; -; Genomic_DNA
EMBLAK314700; BAF98793.1; -; mRNA
EMBLDQ314870; ABC40729.1; -; Genomic_DNA
EMBLBC107587; AAI07588.1; -; mRNA
EMBLBC121125; AAI21126.1; -; mRNA
EMBLM13192; -; NOT_ANNOTATED_CDS; mRNA
EMBLAC004682; AAC27432.1; -; Genomic_DNA
EMBLX00606; CAA25248.1; -; Genomic_DNA
IPIIPI00641737; -; .
PIRA92532; HPHU2; .
PIRA93521; HPHU1; .
RefSeqNP_001119574.1; NM_001126102.1; .
RefSeqNP_005134.1; NM_005143.3; .
UniGeneHs.513711; -; .
UniGeneHs.708058; -; .
ProteinModelPortalP00738; -; .
SMRP00738; 31-406; .
IntActP00738; 5; .
STRINGP00738; -; .
MEROPSS01.972; -; .
SWISS-2DPAGEP00738; -; .
Cornea-2DPAGEP00738; -; .
DOSAC-COBS-2DPAGEP00738; -; .
Siena-2DPAGEP00738; -; .
PRIDEP00738; -; .
EnsemblENST00000355906; ENSP00000348170; ENSG00000197711; .
GeneID3240; -; .
KEGGhsa:3240; -; .
UCSCuc002fbr.2; human; .
CTD3240; -; .
GeneCardsGC16P057856; -; .
H-InvDBHIX0013223; -; .
HGNCHGNC:5141; HP; .
HPACAB003787; -; .
MIM140100; gene; .
neXtProtNX_P00738; -; .
PharmGKBPA29415; -; .
eggNOGprNOG04645; -; .
HOVERGENHBG005989; -; .
InParanoidP00738; -; .
OMALNSEKQW; -; .
OrthoDBEOG4XPQG5; -; .
Pathway_Interaction_DBamb2_neutrophils_pathway; amb2 Integrin signaling; .
NextBio12895; -; .
ArrayExpressP00738; -; .
BgeeP00738; -; .
CleanExHS_HP; -; .
GenevestigatorP00738; -; .
GermOnlineENSG00000197711; Homo sapiens; .
GOGO:0005576; C:extracellular region; NAS:UniProtKB; .
GOGO:0031838; C:haptoglobin-hemoglobin complex; IDA:BHF-UCL; .
GOGO:0030492; F:hemoglobin binding; IDA:BHF-UCL; .
GOGO:0004252; F:serine-type endopeptidase activity; IEA:InterPro; .
GOGO:0006879; P:cellular iron ion homeostasis; NAS:UniProtKB; .
GOGO:0006952; P:defense response; TAS:ProtInc; .
GOGO:2000296; P:negative regulation of hydrogen peroxide catabolic process; IDA:BHF-UCL; .
GOGO:0051354; P:negative regulation of oxidoreductase activity; IDA:BHF-UCL; .
GOGO:0010942; P:positive regulation of cell death; IDA:BHF-UCL; .
GOGO:0006508; P:proteolysis; IEA:InterPro; .
GOGO:0042542; P:response to hydrogen peroxide; IDA:BHF-UCL; .
InterProIPR016060; Complement_control_module; .
InterProIPR009003; Pept_cys/ser_Trypsin-like; .
InterProIPR001254; Peptidase_S1_S6; .
InterProIPR001314; Peptidase_S1A; .
InterProIPR000436; Sushi_SCR_CCP; .
Gene3DG3DSA:2.10.70.10; Complement_control_module; 2; .
PfamPF00089; Trypsin; 1; .
PRINTSPR00722; CHYMOTRYPSIN; .
SMARTSM00032; CCP; 2; .
SMARTSM00020; Tryp_SPc; 1; .
SUPFAMSSF57535; Complement_control_module; 2; .
SUPFAMSSF50494; Pept_Ser_Cys; 1; .
PROSITEPS50923; SUSHI; 2; .
PROSITEPS50240; TRYPSIN_DOM; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server