World-2DPAGE Home


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P02647




SWISS-2DPAGE:  P02647


P02647


General information about the entry
View entry in simple text format
Entry nameAPOA1_HUMAN
Primary accession numberP02647
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 16)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein A-I; Short=Apo-AI; Short=ApoA-I; AltName: Full=Apolipoprotein A1; Contains: RecName: Full=Apolipoprotein A-I(1-242); Flags: Precursor;.
Gene nameName=APOA1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=78094420; PubMed=271964; [NCBI, ExPASy, EBI, Israel, Japan]
Anderson N.L., Anderson N.G.
''''''High resolution two-dimensional electrophoresis of human plasma proteins'';'';''
Proc. Natl. Acad. Sci. U.S.A. 74(1):5421-5425(1977)
[4]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
Comments
  • SUBUNIT: SINGLE CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CSF_HUMAN {Cerebrospinal Fluid}
Homo sapiens (Human)
Tissue: Cerebrospinal fluid
CSF_HUMAN
  map experimental info
  protein estimated location
 
CSF_HUMAN

MAP LOCATIONS:
pI=5.47; Mw=23322
pI=5.05; Mw=23264
pI=5.12; Mw=23206
pI=5.22; Mw=23149

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=5.15; Mw=22770

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [4].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=5.22; Mw=23900

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2SP_HUMAN {HepG2 Secreted Proteins}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2SP_HUMAN
  map experimental info
  protein estimated location
 
HEPG2SP_HUMAN

MAP LOCATIONS:
pI=5.42; Mw=21307
pI=5.34; Mw=21307
pI=5.24; Mw=21062
pI=5.10; Mw=21062

MAPPING (identification):
MATCHING WITH THE PLASMA MASTER GEL [2].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.21; Mw=23862

POSITIONAL VARIANTS:
MILANO, MARBURG, FUKUOKA, NORWAY, GIESSEN, MUNSTER AND IOWA A-1 VARIANTS.

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=5.28; Mw=23247

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



PLASMA_HUMAN {Plasma}
Homo sapiens (Human)
Tissue: Plasma
PLASMA_HUMAN
  map experimental info
  protein estimated location
 
PLASMA_HUMAN

MAP LOCATIONS:
pI=5.22; Mw=23000
pI=5.48; Mw=23453
pI=5.12; Mw=22873
pI=5.05; Mw=22937
pI=4.99; Mw=22873
pI=4.79; Mw=9451
pI=4.86; Mw=9198
pI=6.98; Mw=7695
pI=7.27; Mw=7490

NORMAL LEVEL:
900-2100 MG/L.

PATHOLOGICAL LEVEL:
DECREASED DURING TANGIER DISEASE AND ARTERIOSCLEROSIS; INCREASED DURING LIVER DISEASE.

POSITIONAL VARIANTS:
MILANO, MARBURG, FUKUOKA, NORWAY, GIESSEN, MUNSTER AND IOWA A-1 VARIANTS.

MAPPING (identification):
IMMUNOBLOTING [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP02647; P02647.
DOSAC-COBS-2DPAGEP02647; APOA1_HUMAN.
HSC-2DPAGEP02647; HUMAN.
OGP-WWWP02647.
PMMA-2DPAGEP02647.
REPRODUCTION-2DPAGEP02647; P02647.
REPRODUCTION-2DPAGEIPI00021841; IPI00021841.
Siena-2DPAGEP02647; APOA1_HUMAN.
UCD-2DPAGEP02647; APOA1_HUMAN.
UniProtKB/Swiss-ProtP02647; APOA1_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameAPOA1_HUMAN
Primary accession numberP02647
Secondary accession number(s) A8K866 Q6LDN9 Q6Q785 Q9UCS8 Q9UCT8
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 164)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein A-I; Short=Apo-AI; Short=ApoA-I; AltName: Full=Apolipoprotein A1; Contains: RecName: Full=Apolipoprotein A-I(1-242); Flags: Precursor;
Gene nameName=APOA1
Encoded onName=APOA1
Keywords3D-structure; Amyloid; Amyloidosis; Atherosclerosis; Cholesterol metabolism; Complete proteome; Direct protein sequencing; Disease mutation; Glycation; Glycoprotein; HDL; Lipid metabolism; Lipid transport; Lipoprotein; Neuropathy; Palmitate; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Secreted; Signal; Steroid metabolism; Transport.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ00098; AAB59514.1; -; Genomic_DNA
EMBLX01038; CAA25519.1; -; Genomic_DNA
EMBLX02162; CAA26097.1; -; mRNA
EMBLX00566; CAA25232.1; -; mRNA
EMBLM11791; AAA35545.1; -; mRNA
EMBLX07496; CAA30377.1; -; Genomic_DNA
EMBLM27875; AAA62829.1; -; mRNA
EMBLM29068; AAA51747.1; -; mRNA
EMBLAY422952; AAQ91811.1; -; Genomic_DNA
EMBLAY555191; AAS68227.1; -; Genomic_DNA
EMBLA14829; CAA01198.1; -; mRNA
EMBLAK292231; BAF84920.1; -; mRNA
EMBLEF444948; ACA05932.1; -; Genomic_DNA
EMBLEF444948; ACA05933.1; -; Genomic_DNA
EMBLEF444948; ACA05934.1; -; Genomic_DNA
EMBLEF444948; ACA05935.1; -; Genomic_DNA
EMBLEF444948; ACA05936.1; -; Genomic_DNA
EMBLCH471065; EAW67274.1; -; Genomic_DNA
EMBLBC005380; AAH05380.1; -; mRNA
EMBLBC110286; AAI10287.1; -; mRNA
IPIIPI00021841; -; .
PIRA90947; LPHUA1; .
RefSeqNP_000030.1; NM_000039.1; .
UniGeneHs.93194; -; .
PDB1AV1; X-ray; 4.00 A; A/B/C/D=68-267
PDB1GW3; NMR; -; A=166-211
PDB1GW4; NMR; -; A=166-211
PDB1ODP; NMR; -; A=190-209
PDB1ODQ; NMR; -; A=190-209
PDB1ODR; NMR; -; A=190-209
PDB2A01; X-ray; 2.40 A; A/B/C=25-267
PDB3J00; EM; -; 0/1=68-267
PDB3K2S; X-ray; -; A/B=25-267
PDBsum1AV1; -; .
PDBsum1GW3; -; .
PDBsum1GW4; -; .
PDBsum1ODP; -; .
PDBsum1ODQ; -; .
PDBsum1ODR; -; .
PDBsum2A01; -; .
PDBsum3J00; -; .
PDBsum3K2S; -; .
ProteinModelPortalP02647; -; .
SMRP02647; 68-267; .
DisProtDP00386; -; .
DIPDIP-29619N; -; .
IntActP02647; 49; .
MINTMINT-5000866; -; .
STRINGP02647; -; .
PhosphoSiteP02647; -; .
SWISS-2DPAGEP02647; -; .
Cornea-2DPAGEP02647; -; .
DOSAC-COBS-2DPAGEP02647; -; .
OGPP02647; -; .
PMMA-2DPAGEP02647; -; .
REPRODUCTION-2DPAGEIPI00021841; -; .
REPRODUCTION-2DPAGEP02647; -; .
Siena-2DPAGEP02647; -; .
UCD-2DPAGEP02647; -; .
PeptideAtlasP02647; -; .
PRIDEP02647; -; .
EnsemblENST00000236850; ENSP00000236850; ENSG00000118137; .
EnsemblENST00000359492; ENSP00000352471; ENSG00000118137; .
EnsemblENST00000375320; ENSP00000364469; ENSG00000118137; .
EnsemblENST00000375323; ENSP00000364472; ENSG00000118137; .
GeneID335; -; .
KEGGhsa:335; -; .
UCSCuc001ppu.1; human; .
CTD335; -; .
GeneCardsGC11M112638; -; .
HGNCHGNC:600; APOA1; .
HPACAB016778; -; .
MIM105200; phenotype; .
MIM107680; gene; .
MIM205400; phenotype; .
MIM604091; phenotype; .
neXtProtNX_P02647; -; .
Orphanet425; Apolipoprotein A-I deficiency; .
Orphanet93560; Familial renal amyloidosis due to Apolipoprotein AI variant; .
Orphanet85443; Primary amyloidosis; .
PharmGKBPA49; -; .
eggNOGprNOG20905; -; .
HOGENOMHBG280675; -; .
HOVERGENHBG105708; -; .
InParanoidP02647; -; .
OMAYRQKVAP; -; .
OrthoDBEOG44J2JV; -; .
PhylomeDBP02647; -; .
Pathway_Interaction_DBhnf3bpathway; FOXA2 and FOXA3 transcription factor networks; .
ReactomeREACT_15518; Transmembrane transport of small molecules; .
ReactomeREACT_22258; Metabolism of lipids and lipoproteins; .
ReactomeREACT_604; Hemostasis; .
ReactomeREACT_75925; Amyloids; .
NextBio1387; -; .
PMAP-CutDBP02647; -; .
ArrayExpressP02647; -; .
BgeeP02647; -; .
CleanExHS_APOA1; -; .
GenevestigatorP02647; -; .
GermOnlineENSG00000118137; Homo sapiens; .
GOGO:0030139; C:endocytic vesicle; IDA:BHF-UCL; .
GOGO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome; .
GOGO:0005886; C:plasma membrane; TAS:Reactome; .
GOGO:0034366; C:spherical high-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0030141; C:stored secretory granule; TAS:Reactome; .
GOGO:0034361; C:very-low-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0034191; F:apolipoprotein A-I receptor binding; IPI:BHF-UCL; .
GOGO:0001540; F:beta-amyloid binding; IDA:BHF-UCL; .
GOGO:0015485; F:cholesterol binding; IDA:BHF-UCL; .
GOGO:0017127; F:cholesterol transporter activity; IMP:BHF-UCL; .
GOGO:0019899; F:enzyme binding; IPI:BHF-UCL; .
GOGO:0070653; F:high-density lipoprotein particle receptor binding; IPI:BHF-UCL; .
GOGO:0042802; F:identical protein binding; IPI:BHF-UCL; .
GOGO:0060228; F:phosphatidylcholine-sterol O-acyltransferase activator activity; IDA:BHF-UCL; .
GOGO:0005543; F:phospholipid binding; IDA:BHF-UCL; .
GOGO:0032488; P:Cdc42 protein signal transduction; IDA:BHF-UCL; .
GOGO:0033344; P:cholesterol efflux; IDA:BHF-UCL; .
GOGO:0042632; P:cholesterol homeostasis; IMP:BHF-UCL; .
GOGO:0070508; P:cholesterol import; IMP:BHF-UCL; .
GOGO:0008203; P:cholesterol metabolic process; IMP:BHF-UCL; .
GOGO:0007186; P:G-protein coupled receptor protein signaling pathway; IDA:BHF-UCL; .
GOGO:0034380; P:high-density lipoprotein particle assembly; IDA:BHF-UCL; .
GOGO:0034384; P:high-density lipoprotein particle clearance; IC:BHF-UCL; .
GOGO:0034375; P:high-density lipoprotein particle remodeling; IC:BHF-UCL; .
GOGO:0042157; P:lipoprotein metabolic process; TAS:Reactome; .
GOGO:0002740; P:negative regulation of cytokine secretion involved in immune response; IDA:BHF-UCL; .
GOGO:0050713; P:negative regulation of interleukin-1 beta secretion; IDA:BHF-UCL; .
GOGO:0010903; P:negative regulation of very-low-density lipoprotein particle remodeling; IDA:BHF-UCL; .
GOGO:0006656; P:phosphatidylcholine biosynthetic process; IDA:BHF-UCL; .
GOGO:0033700; P:phospholipid efflux; IDA:BHF-UCL; .
GOGO:0030168; P:platelet activation; TAS:Reactome; .
GOGO:0002576; P:platelet degranulation; TAS:Reactome; .
GOGO:0010873; P:positive regulation of cholesterol esterification; IDA:BHF-UCL; .
GOGO:0051345; P:positive regulation of hydrolase activity; IDA:BHF-UCL; .
GOGO:0050821; P:protein stabilization; IDA:BHF-UCL; .
GOGO:0043691; P:reverse cholesterol transport; IMP:BHF-UCL; .
InterProIPR013326; ApoA/E_ApoLp; .
InterProIPR000074; ApoA1_A4_E; .
Gene3DG3DSA:1.20.120.20; ApoA/E_ApoLp; 1; .
PfamPF01442; Apolipoprotein; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server