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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P04406




SWISS-2DPAGE:  P04406


P04406


General information about the entry
View entry in simple text format
Entry nameG3P_HUMAN
Primary accession numberP04406
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 16)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;.
Gene nameName=GAPDH
Synonyms=GAPD
ORFNames=CDABP0047, OK/SW-cl.12
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.
''''''Human liver protein map: update 1993'';'';''
Electrophoresis 14(1):1216-1222(1993)
[2]   MAPPING ON GEL
MEDLINE=93120196; PubMed=1477117; [NCBI, ExPASy, EBI, Israel, Japan]
Golaz O., Walzer C., Hochstrasser D.F., Bjellqvist B., Turler H., Balant L.P.
''''''Red blood cell protein map: a comparison between carrier-ampholyte pH gradient and immobilized pH gradient, and identification of four red blood cell enzymes'';'';''
Appl. Theor. Electrophor. 3(1):77-82(1992)
[3]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[4]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''''''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis'';'';''
Electrophoresis 16(1):1152-1159(1995)
Comments
  • SUBUNIT: HOMOTETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=8.59; Mw=35598
pI=8.49; Mw=35518
pI=8.22; Mw=35198

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=9.24; Mw=35278
pI=8.99; Mw=35057
pI=8.48; Mw=34984
pI=8.28; Mw=34984
pI=8.74; Mw=34837

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=8.73; Mw=35173
pI=8.10; Mw=35000
pI=8.42; Mw=35115
pI=7.83; Mw=34841
pI=7.61; Mw=34762

MAPPING (identification):
MATCHING WITH RBC AND LIVER MASTERS [4].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=9.65; Mw=34926
pI=9.41; Mw=34783
pI=9.15; Mw=34569
pI=8.96; Mw=34428
pI=8.70; Mw=34287
pI=8.45; Mw=34147

MAPPING (identification):
COMIGRATION [2].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=8.95; Mw=35643
pI=8.69; Mw=35414
pI=7.91; Mw=35300
pI=8.24; Mw=35243

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP04406; P04406.
DOSAC-COBS-2DPAGEP04406; G3P_HUMAN.
HSC-2DPAGEP04406; HUMAN.
OGP-WWWP04406.
REPRODUCTION-2DPAGEP04406; P04406.
REPRODUCTION-2DPAGEIPI00219018; IPI00219018.
Siena-2DPAGEP04406; G3P_HUMAN.
UCD-2DPAGEP04406; G3P_HUMAN.
UniProtKB/Swiss-ProtP04406; G3P_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameG3P_HUMAN
Primary accession numberP04406
Secondary accession number(s) P00354 Q53X65
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on October 19, 2011 (version 163)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;
Gene nameName=GAPDH
Synonyms=GAPD
ORFNames=CDABP0047, OK/SW-cl.12
Encoded onName=GAPDH; Synonyms=GAPD; ORFNames=CDABP0047, OK/SW-cl.12
Keywords3D-structure; Acetylation; ADP-ribosylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Membrane; Methylation; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; S-nitrosylation; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX01677; CAA25833.1; -; mRNA
EMBLM17851; AAA86283.1; -; mRNA
EMBLM33197; AAA52518.1; -; mRNA
EMBLJ02642; AAA52496.1; -; mRNA
EMBLJ04038; AAA53191.1; -; Genomic_DNA
EMBLX53778; CAA37794.1; -; mRNA
EMBLAF261085; AAF99678.1; -; mRNA
EMBLAY007133; AAG01996.1; -; mRNA
EMBLAB062273; BAB93466.1; -; mRNA
EMBLBT006893; AAP35539.1; -; mRNA
EMBLAY340484; AAP88932.1; -; Genomic_DNA
EMBLCR407671; CAG28599.1; -; mRNA
EMBLAC006064; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLCH471116; EAW88787.1; -; Genomic_DNA
EMBLBC001601; AAH01601.1; -; mRNA
EMBLBC004109; AAH04109.1; -; mRNA
EMBLBC009081; AAH09081.1; -; mRNA
EMBLBC013310; AAH13310.1; -; mRNA
EMBLBC023632; AAH23632.1; -; mRNA
EMBLBC025925; AAH25925.1; -; mRNA
EMBLBC026907; AAH26907.1; -; mRNA
EMBLBC029618; AAH29618.1; -; mRNA
EMBLBC083511; AAH83511.1; -; mRNA
IPIIPI00219018; -; .
PIRA31988; DEHUG3; .
RefSeqNP_002037.2; NM_002046.3; .
UniGeneHs.544577; -; .
UniGeneHs.592355; -; .
UniGeneHs.598320; -; .
PDB1U8F; X-ray; 1.75 A; O/P/Q/R=1-335
PDB1ZNQ; X-ray; 2.50 A; O/P/Q/R=1-335
PDB2FEH; Model; -; O/P/Q/R=1-335
PDB3GPD; X-ray; 3.50 A; G/R=2-335
PDBsum1U8F; -; .
PDBsum1ZNQ; -; .
PDBsum2FEH; -; .
PDBsum3GPD; -; .
ProteinModelPortalP04406; -; .
SMRP04406; 2-335; .
IntActP04406; 32; .
MINTMINT-1150338; -; .
STRINGP04406; -; .
PhosphoSiteP04406; -; .
SWISS-2DPAGEP04406; -; .
Aarhus/Ghent-2DPAGE1206; NEPHGE; .
Cornea-2DPAGEP04406; -; .
DOSAC-COBS-2DPAGEP04406; -; .
OGPP04406; -; .
REPRODUCTION-2DPAGEIPI00219018; -; .
REPRODUCTION-2DPAGEP04406; -; .
Siena-2DPAGEP04406; -; .
UCD-2DPAGEP04406; -; .
PRIDEP04406; -; .
EnsemblENST00000229239; ENSP00000229239; ENSG00000111640; .
EnsemblENST00000396859; ENSP00000380068; ENSG00000111640; .
EnsemblENST00000396861; ENSP00000380070; ENSG00000111640; .
EnsemblENST00000406765; ENSP00000384819; ENSG00000111640; .
GeneID2597; -; .
KEGGhsa:2597; -; .
UCSCuc001qop.1; human; .
CTD2597; -; .
GeneCardsGC12P006514; -; .
H-InvDBHIX0010367; -; .
HGNCHGNC:4141; GAPDH; .
HPACAB005197; -; .
HPACAB016392; -; .
MIM138400; gene; .
neXtProtNX_P04406; -; .
PharmGKBPA28554; -; .
eggNOGprNOG07879; -; .
HOGENOMHBG571736; -; .
HOVERGENHBG000227; -; .
InParanoidP04406; -; .
OMAKEICTEV; -; .
OrthoDBEOG4Q84XS; -; .
PhylomeDBP04406; -; .
BRENDA1.2.1.12; 2681; .
ReactomeREACT_474; Metabolism of carbohydrates; .
DrugBankDB00157; NADH; .
NextBio10271; -; .
ArrayExpressP04406; -; .
BgeeP04406; -; .
CleanExHS_GAPDH; -; .
GenevestigatorP04406; -; .
GermOnlineENSG00000111640; Homo sapiens; .
GOGO:0005829; C:cytosol; ISS:UniProtKB; .
GOGO:0016020; C:membrane; IEA:UniProtKB-SubCell; .
GOGO:0005634; C:nucleus; ISS:UniProtKB; .
GOGO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; ISS:UniProtKB; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0035605; F:peptidyl-cysteine S-nitrosylase activity; ISS:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:UniProtKB; .
GOGO:0006094; P:gluconeogenesis; TAS:Reactome; .
GOGO:0006096; P:glycolysis; NAS:UniProtKB; .
GOGO:0051402; P:neuron apoptosis; ISS:UniProtKB; .
GOGO:0035606; P:peptidyl-cysteine S-trans-nitrosylation; ISS:UniProtKB; .
GOGO:0050821; P:protein stabilization; ISS:UniProtKB; .
InterProIPR020831; GlycerAld/Erythrose_P_DH; .
InterProIPR020830; GlycerAld_3-P_DH_AS; .
InterProIPR020829; GlycerAld_3-P_DH_cat; .
InterProIPR020828; GlycerAld_3-P_DH_NAD(P)-bd; .
InterProIPR006424; Glyceraldehyde-3-P_DH_1; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PANTHERPTHR10836; GAP_DH; 1; .
PfamPF02800; Gp_dh_C; 1; .
PfamPF00044; Gp_dh_N; 1; .
PIRSFPIRSF000149; GAP_DH; 1; .
PRINTSPR00078; G3PDHDRGNASE; .
SMARTSM00846; Gp_dh_N; 1; .
TIGRFAMsTIGR01534; GAPDH-I; 1; .
PROSITEPS00071; GAPDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server