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Sample Preparation and Post-separation Analysis

Searching in 'SWISS-2DPAGE' for entry matching: P06748



General information about the entry
View entry in simple text format
Entry nameNPM_HUMAN
Primary accession numberP06748
integrated into SWISS-2DPAGE on January 15, 1999 (release 9)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Nucleophosmin; Short=NPM; AltName: Full=Nucleolar phosphoprotein B23; AltName: Full=Nucleolar protein NO38; AltName: Full=Numatrin;.
Gene nameName=NPM1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
2D PAGE maps for identified proteins
How to interpret a protein

NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
  map experimental info
  protein estimated location

Mw=41199  [identification data]
Mw=40297  [identification data]
Mw=39648  [identification data]
Mw=38781  [identification data]
Mw=38269  [identification data]
Mw=37709  [identification data]
Mw=37156  [identification data]
Mw=35286  [identification data]
Mw=30626  [identification data]
Mw=24176  [identification data]
Mw=22406  [identification data]
Mw=20822  [identification data]
Mw=20347  [identification data]

MAPPING (identification):
Tandem mass spectrometry [2]; BANDS 1V91, 1V93: PEPTIDE MASS FINGERPRINTING [2].

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
  map experimental info
  protein estimated location

pI=4.77; Mw=35011  [identification data]
pI=4.82; Mw=34718  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [1].

NUCLEOLI_HELA_2D_HUMAN {2D-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
  map experimental info
  protein estimated location

pI=4.79; Mw=38894  [identification data]
pI=4.68; Mw=38388  [identification data]
pI=4.79; Mw=37314  [identification data]
pI=4.65; Mw=36910  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2];

This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See or send email from
DOSAC-COBS 2D-PAGEP06748; P06748.
UniProtKB/Swiss-ProtP06748; NPM_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameNPM_HUMAN
Primary accession numberP06748
Secondary accession number(s) A8K3N7 B5BU00 D3DQL6 P08693 Q12826 Q13440 Q13441 Q14115 Q5EU94 Q5EU95 Q5EU96 Q5EU97 Q5EU98 Q5EU99 Q6V962 Q8WTW5 Q96AT6 Q96DC4 Q96EA5 Q9UDJ7
Sequence was last modified on November 1, 1990 (version 2)
Annotations were last modified on October 19, 2011 (version 150)
Name and origin of the protein
DescriptionRecName: Full=Nucleophosmin; Short=NPM; AltName: Full=Nucleolar phosphoprotein B23; AltName: Full=Nucleolar protein NO38; AltName: Full=Numatrin;
Gene nameName=NPM1
Encoded onName=NPM1; Synonyms=NPM
Keywords3D-structure; Acetylation; ADP-ribosylation; Alternative splicing; Chaperone; Chromosomal rearrangement; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disulfide bond; Host-virus interaction; Isopeptide bond; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; RNA-binding; Ubl conjugation.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLM23613; AAA36380.1; -; mRNA
EMBLM28699; AAA58386.1; -; mRNA
EMBLM26697; AAA36385.1; -; mRNA
EMBLU89321; AAB94739.1; -; Genomic_DNA
EMBLU89309; AAB94739.1; JOINED; Genomic_DNA
EMBLU89310; AAB94739.1; JOINED; Genomic_DNA
EMBLU89311; AAB94739.1; JOINED; Genomic_DNA
EMBLU89313; AAB94739.1; JOINED; Genomic_DNA
EMBLU89314; AAB94739.1; JOINED; Genomic_DNA
EMBLU89317; AAB94739.1; JOINED; Genomic_DNA
EMBLU89319; AAB94739.1; JOINED; Genomic_DNA
EMBLAY740634; AAW67752.1; -; mRNA
EMBLAY740635; AAW67753.1; -; mRNA
EMBLAY740636; AAW67754.1; -; mRNA
EMBLAY740637; AAW67755.1; -; mRNA
EMBLAY740638; AAW67756.1; -; mRNA
EMBLAY740639; AAW67757.1; -; mRNA
EMBLAY740640; AAW67758.1; -; mRNA
EMBLDQ303464; ABC40399.1; -; mRNA
EMBLAY347529; AAQ24860.1; -; mRNA
EMBLBT007011; AAP35657.1; -; mRNA
EMBLAK290652; BAF83341.1; -; mRNA
EMBLAB451236; BAG70050.1; -; mRNA
EMBLAB451361; BAG70175.1; -; mRNA
EMBLCH471062; EAW61443.1; -; Genomic_DNA
EMBLCH471062; EAW61446.1; -; Genomic_DNA
EMBLBC002398; AAH02398.1; -; mRNA
EMBLBC008495; AAH08495.1; -; mRNA
EMBLBC009623; AAH09623.1; -; mRNA
EMBLBC012566; AAH12566.1; -; mRNA
EMBLBC014349; AAH14349.1; -; mRNA
EMBLBC016716; AAH16716.1; -; mRNA
EMBLBC016768; AAH16768.1; -; mRNA
EMBLBC016824; AAH16824.1; -; mRNA
EMBLBC021668; AAH21668.1; -; mRNA
EMBLBC021983; AAH21983.1; -; mRNA
EMBLBC050628; AAH50628.1; -; mRNA
EMBLU41742; AAB00112.1; ALT_TERM; mRNA
EMBLU41743; AAB00113.1; ALT_TERM; mRNA
EMBLU04946; AAA58698.1; ALT_TERM; mRNA
EMBLD45915; BAA08343.1; ALT_TERM; mRNA
EMBLX16934; CAA34809.1; -; mRNA
EMBLJ02590; AAA36473.1; -; mRNA
EMBLM31004; AAA36474.1; -; mRNA
IPIIPI00220740; -; .
IPIIPI00549248; -; .
PIRA33423; A32915; .
PIRI38491; I38491; .
RefSeqNP_002511.1; NM_002520.6; .
RefSeqNP_954654.1; NM_199185.3; .
UniGeneHs.557550; -; .
PDB2P1B; X-ray; 2.75 A; A/B/C/D/E/F/G/H/I/J=9-122
PDB2VXD; NMR; -; A=243-294
PDBsum2P1B; -; .
PDBsum2VXD; -; .
ProteinModelPortalP06748; -; .
SMRP06748; 15-118; 243-294; .
IntActP06748; 39; .
MINTMINT-4999760; -; .
STRINGP06748; -; .
PhosphoSiteP06748; -; .
SWISS-2DPAGEP06748; -; .
PRIDEP06748; -; .
EnsemblENST00000296930; ENSP00000296930; ENSG00000181163; .
GeneID4869; -; .
KEGGhsa:4869; -; .
UCSCuc003mbi.1; human; .
UCSCuc003mbj.1; human; .
CTD4869; -; .
GeneCardsGC05P165912; -; .
H-InvDBHIX0005411; -; .
HGNCHGNC:7910; NPM1; .
HPACAB012983; -; .
HPAHPA011384; -; .
MIM164040; gene; .
neXtProtNX_P06748; -; .
Orphanet520; Acute promyelocytic leukemia; .
PharmGKBPA31712; -; .
eggNOGprNOG07913; -; .
HOVERGENHBG001860; -; .
InParanoidP06748; -; .
OrthoDBEOG4W3SNZ; -; .
PhylomeDBP06748; -; .
Pathway_Interaction_DBaurora_b_pathway; Aurora B signaling; .
Pathway_Interaction_DBbard1pathway; BARD1 signaling events; .
Pathway_Interaction_DBhif1_tfpathway; HIF-1-alpha transcription factor network; .
ReactomeREACT_22172; Chromosome Maintenance; .
ReactomeREACT_6185; HIV Infection; .
NextBio18752; -; .
PMAP-CutDBP06748; -; .
ArrayExpressP06748; -; .
BgeeP06748; -; .
GenevestigatorP06748; -; .
GermOnlineENSG00000181163; Homo sapiens; .
GOGO:0005730; C:nucleolus; IDA:UniProtKB; .
GOGO:0005654; C:nucleoplasm; IDA:UniProtKB; .
GOGO:0030529; C:ribonucleoprotein complex; IDA:MGI; .
GOGO:0031616; C:spindle pole centrosome; IDA:UniProtKB; .
GOGO:0042393; F:histone binding; IDA:UniProtKB; .
GOGO:0051059; F:NF-kappaB binding; IDA:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0046982; F:protein heterodimerization activity; IMP:UniProtKB; .
GOGO:0042803; F:protein homodimerization activity; IDA:UniProtKB; .
GOGO:0043023; F:ribosomal large subunit binding; IDA:MGI; .
GOGO:0043024; F:ribosomal small subunit binding; IDA:MGI; .
GOGO:0003723; F:RNA binding; IDA:UniProtKB; .
GOGO:0030957; F:Tat protein binding; IDA:UniProtKB; .
GOGO:0003713; F:transcription coactivator activity; IDA:UniProtKB; .
GOGO:0051082; F:unfolded protein binding; IDA:UniProtKB; .
GOGO:0006916; P:anti-apoptosis; NAS:UniProtKB; .
GOGO:0007569; P:cell aging; IMP:UniProtKB; .
GOGO:0034080; P:CenH3-containing nucleosome assembly at centromere; TAS:Reactome; .
GOGO:0007098; P:centrosome cycle; IMP:UniProtKB; .
GOGO:0006281; P:DNA repair; IDA:UniProtKB; .
GOGO:0044419; P:interspecies interaction between organisms; IEA:UniProtKB-KW; .
GOGO:0006886; P:intracellular protein transport; TAS:UniProtKB; .
GOGO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB; .
GOGO:0010826; P:negative regulation of centrosome duplication; IMP:UniProtKB; .
GOGO:0006913; P:nucleocytoplasmic transport; IDA:UniProtKB; .
GOGO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IMP:UniProtKB; .
GOGO:0051259; P:protein oligomerization; IDA:MGI; .
GOGO:0032071; P:regulation of endodeoxyribonuclease activity; IDA:UniProtKB; .
GOGO:0060699; P:regulation of endoribonuclease activity; IDA:UniProtKB; .
GOGO:0042255; P:ribosome assembly; TAS:UniProtKB; .
GOGO:0007165; P:signal transduction; NAS:UniProtKB; .
InterProIPR004301; Nucleoplasmin; .
InterProIPR024057; Nucleoplasmin_core; .
Gene3DG3DSA:; G3DSA:; 1; .
PANTHERPTHR22747; Nucleoplasmin; 1; .
PfamPF03066; Nucleoplasmin; 1; .
SUPFAMSSF69203; Nucleoplasmin; 1; .


SWISS-2DPAGE (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server