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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: P09158 |
SWISS-2DPAGE: P09158
P09158:
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ID SPEE_ECOLI; STANDARD; 2DG.
AC P09158;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 7.
DE RecName: Full=Spermidine synthase; EC=2.5.1.16; AltName: Full=Putrescine
DE aminopropyltransferase; Short=PAPT; AltName: Full=SPDSY;.
GN Name=speE; OrderedLocusNames=b0121, JW0117;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WRB=5.30/32000;
2D -!- PEPTIDE MASSES: SPOT 2D-001WRB: 1167.6; 1408.74; 1418.75; 1746.77;
2D 1846.93; 2021.96; 2850.44; 3059.5; 3187.64; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WRB: (K)TDHQDLIIFENAAFGR(V),29-44; (R)CLNPGGIFVAQNGVCFLQQEEAIDSHR,180-206.
2D -!- EXPRESSION: decrease after benzoic acid treatment [1].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P09158; SPEE_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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