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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6A6




SWISS-2DPAGE:  P0A6A6


P0A6A6


General information about the entry
View entry in simple text format
Entry nameLEUC_ECOLI
Primary accession numberP0A6A6
Secondary accession number(s) P30127
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onMarch 31, 2004 (version 3)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=3-isopropylmalate dehydratase large subunit; EC=4.2.1.33; AltName: Full=Alpha-IPM isomerase; Short=IPMI; AltName: Full=Isopropylmalate isomerase;.
Gene nameName=leuC
OrderedLocusNames=b0072, JW0071
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[2]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.95; Mw=51735
pI=5.77; Mw=51532  [identification data]
pI=5.42; Mw=44527

MAPPING (identification):
AMINO ACID COMPOSITION AND SEQUENCE TAG (AKTL) [1].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.93; Mw=50340  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [2].

MAPPING (identification):
Peptide mass fingerprinting [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP0A6A6; LEUC_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameLEUC_ECOLI
Primary accession numberP0A6A6
Secondary accession number(s) P30127 P78042 Q8FL77
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 58)
Name and origin of the protein
DescriptionRecName: Full=3-isopropylmalate dehydratase large subunit; EC=4.2.1.33; AltName: Full=Alpha-IPM isomerase; Short=IPMI; AltName: Full=Isopropylmalate isomerase;
Gene nameName=leuC
OrderedLocusNames=b0072, JW0071
Encoded onName=leuC; OrderedLocusNames=b0072, JW0071
Keywords4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU00096; AAC73183.1; -; Genomic_DNA
EMBLAP009048; BAB96641.2; -; Genomic_DNA
EMBLD17631; BAA21004.1; -; Genomic_DNA
EMBLD17632; BAA21005.1; -; Genomic_DNA
PIRH64728; H64728; .
RefSeqNP_414614.1; NC_000913.2; .
ProteinModelPortalP0A6A6; -; .
SMRP0A6A6; 2-462; .
DIPDIP-35834N; -; .
IntActP0A6A6; 2; .
SWISS-2DPAGEP0A6A6; -; .
EnsemblBacteriaEBESCT00000002539; EBESCP00000002539; EBESCG00000002073; .
EnsemblBacteriaEBESCT00000017453; EBESCP00000016744; EBESCG00000016509; .
GeneID945076; -; .
GenomeReviewsAP009048_GR; JW0071; .
GenomeReviewsU00096_GR; b0072; .
KEGGecj:JW0071; -; .
KEGGeco:b0072; -; .
EchoBASEEB1536; -; .
EcoGeneEG11576; leuC; .
eggNOGCOG0065; -; .
GeneTreeEBGT00050000008227; -; .
HOGENOMHBG330745; -; .
OMADIRQGIV; -; .
ProtClustDBPRK05478; -; .
BioCycEcoCyc:LEUC-MONOMER; -; .
BioCycMetaCyc:LEUC-MONOMER; -; .
GenevestigatorP0A6A6; -; .
GOGO:0003861; F:3-isopropylmalate dehydratase activity; IEA:EC; .
GOGO:0051539; F:4 iron; 4 sulfur cluster binding; IEA:UniProtKB-KW
GOGO:0016866; F:intramolecular transferase activity; IDA:EcoCyc; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0009098; P:leucine biosynthetic process; IDA:EcoCyc; .
HAMAPMF_01026; LeuC_type1; 1; -
InterProIPR004430; 3-IsopropMal_deHydase_lsu; .
InterProIPR015931; Acnase/IPM_dHydase_lsu_aba_1/3; .
InterProIPR015937; Acoase/IPM_deHydtase; .
InterProIPR001030; Acoase/IPM_deHydtase_lsu_aba; .
InterProIPR015932; Aconitase/IPMdHydase_lsu_aba_2; .
InterProIPR018136; Aconitase_4Fe-4S_BS; .
InterProIPR015936; Homoacnase/3IPM_deHydtase_su; .
Gene3DG3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 2; .
Gene3DG3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1; .
PANTHERPTHR11670; Aconitase-like_core; 1; .
PANTHERPTHR11670:SF6; Homoacnase/3IPM_dehydase_s/lsu; 1; .
PfamPF00330; Aconitase; 1; .
PRINTSPR00415; ACONITASE; .
SUPFAMSSF53732; Aconitase_N; 1; .
TIGRFAMsTIGR00170; LeuC; 1; .
PROSITEPS00450; ACONITASE_1; 1; .
PROSITEPS01244; ACONITASE_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server