Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P0A6A6:

ID   LEUC_ECOLI; STANDARD; 2DG.
AC   P0A6A6; P30127;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   31-MAR-2004, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 12.
DE   RecName: Full=3-isopropylmalate dehydratase large subunit; EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase; Short=IPMI; AltName:
DE   Full=Isopropylmalate isomerase;.
GN   Name=leuC; OrderedLocusNames=b0072, JW0071;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [2]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KRI=5.95/51735;
2D   -!-   PI/MW: SPOT 2D-000KRM=5.77/51532;
2D   -!-   PI/MW: SPOT 2D-000KX2=5.42/44527;
2D   -!-   AMINO ACID COMPOSITION: SPOT 2D-000KRM: B=9.2, Z=11.1, S=5.9,
2D         H=1.4, G=13.8, T=6.6, A=12, P=4.9, Y=1.4, R=5.3, V=6.6, M=1.9, I=5.2,
2D         L=7.3, F=3.9, K=3.4;
2D   -!-   MAPPING: AMINO ACID COMPOSITION AND SEQUENCE TAG (AKTL) [1].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WIX=5.93/50340;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WIX: 1018.59; 1031.55; 1049.57; 1140.61;
2D         1574.99; 1670.77; 1703.08; 1805.98; 1838.02; 2116.16; TRYPSIN.
2D   -!-   EXPRESSION: decrease after benzoic acid treatment [2].
2D   -!-   MAPPING: Peptide mass fingerprinting [2].
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DR   UniProtKB/Swiss-Prot; P0A6A6; LEUC_ECOLI.
//

The following 2-D maps are available for this protein:

	Escherichia coli	Escherichia coli DIGE (4.5-6.5)

SWISS-2DPAGE (search AC)