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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6P1




SWISS-2DPAGE:  P0A6P1


P0A6P1


General information about the entry
View entry in simple text format
Entry nameEFTS_ECOLI
Primary accession numberP0A6P1
Secondary accession number(s) P02997
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 6)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor Ts; Short=EF-Ts;.
Gene nameName=tsf
OrderedLocusNames=b0170, JW0165
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
[4]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[5]   PROTEIN EXPRESSION
MEDLINE=79216190; PubMed=156716; [NCBI, ExPASy, EBI, Israel, Japan]
Herendeen S.L., Vanbogelen R.A., Neidhardt F.C.
''''''Levels of major proteins of Escherichia coli during growth at different temperatures'';'';''
J. Bacteriol. 139(1):185-194(1979)
[6]   PROTEIN EXPRESSION
MEDLINE=78212912; PubMed=352533; [NCBI, ExPASy, EBI, Israel, Japan]
Pedersen S., Bloch P.L., Reeh S., Neidhardt F.C.
''''''Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates'';'';''
Cell 14(1):179-190(1978)
[7]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[8]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.16; Mw=33686  [identification data]
pI=5.06; Mw=33758  [identification data]

EXPRESSION:
CHANGED VERY LITTLE THROUGHOUT THE NORMAL ARRHENIUS RANGE (23 TO 37°C) [5]; INCREASED IN LEVEL WITH INCREASING GROWTH RATE [6].

MAPPING (identification):
SPOT 2D-000L7K: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3];
SPOT 2D-000L7M: AMINO ACID COMPOSITION AND SEQUENCE TAG (AE) [4].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.22; Mw=32533  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [7].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.14; Mw=34596  [identification data]
pI=5.08; Mw=34633  [identification data]
pI=5.20; Mw=34415  [identification data]

EXPRESSION:
increase after benzoic acid treatment [8].

MAPPING (identification):
SPOTS 1WPB, 1WPG: PEPTIDE MASS FINGERPRINTING [8]; Tandem mass spectrometry [8].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP0A6P1; EFTS_ECOLI.
ECO2DBASEC030.7; 6TH EDITION.
ECO2DBASEC031.6; 6TH EDITION.
UniProtKB/Swiss-ProtP0A6P1; EFTS_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameEFTS_ECOLI
Primary accession numberP0A6P1
Secondary accession number(s) P02997
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 72)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor Ts; Short=EF-Ts;
Gene nameName=tsf
OrderedLocusNames=b0170, JW0165
Encoded onName=tsf; OrderedLocusNames=b0170, JW0165
Keywords3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Protein biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00343; CAA23632.1; -; Genomic_DNA
EMBLD13334; BAA02597.1; -; Genomic_DNA
EMBLU00096; AAC73281.1; -; Genomic_DNA
EMBLAP009048; BAB96746.1; -; Genomic_DNA
EMBLU70214; AAB08599.1; -; Genomic_DNA
PIRA03525; EFECS; .
RefSeqNP_414712.1; NC_000913.2; .
PDB1EFU; X-ray; 2.50 A; B/D=2-283
PDB3MMP; X-ray; 2.50 A; A/C=1-283
PDBsum1EFU; -; .
PDBsum3MMP; -; .
ProteinModelPortalP0A6P1; -; .
SMRP0A6P1; 2-283; .
DIPDIP-31835N; -; .
IntActP0A6P1; 10; .
MINTMINT-1273899; -; .
PhosSiteP0A6P1; -; .
SWISS-2DPAGEP0A6P1; -; .
2DBase-EcoliP0A6P1; -; .
ECO2DBASEC030.7; 6TH EDITION; .
ECO2DBASEC031.6; 6TH EDITION; .
EnsemblBacteriaEBESCT00000002436; EBESCP00000002436; EBESCG00000001985; .
EnsemblBacteriaEBESCT00000016027; EBESCP00000015318; EBESCG00000015087; .
GeneID944866; -; .
GenomeReviewsAP009048_GR; JW0165; .
GenomeReviewsU00096_GR; b0170; .
KEGGecj:JW0165; -; .
KEGGeco:b0170; -; .
EchoBASEEB1026; -; .
EcoGeneEG11033; tsf; .
eggNOGCOG0264; -; .
GeneTreeEBGT00050000009998; -; .
HOGENOMHBG713289; -; .
OMAYLHGTRI; -; .
ProtClustDBPRK09377; -; .
BioCycEcoCyc:EG11033-MONOMER; -; .
GenevestigatorP0A6P1; -; .
GOGO:0005737; C:cytoplasm; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW; .
GOGO:0008270; F:zinc ion binding; IDA:EcoliWiki; .
HAMAPMF_00050; EF_Ts; 1; -
InterProIPR001816; Transl_elong_EFTs/EF1B; .
InterProIPR014039; Transl_elong_EFTs/EF1B_dimer; .
InterProIPR018101; Transl_elong_Ts_CS; .
InterProIPR009060; UBA-like; .
InterProIPR000449; UBA/transl_elong_EF1B_N; .
Gene3DG3DSA:3.30.479.20; Transl_elong_EFTs/EF1B_dimer; 3; .
PANTHERPTHR11741; Transl_elong_EFTs/EF1B; 1; .
PfamPF00889; EF_TS; 1; .
PfamPF00627; UBA; 1; .
SUPFAMSSF54713; EF_TS; 2; .
SUPFAMSSF46934; UBA_like; 1; .
TIGRFAMsTIGR00116; Tsf; 1; .
PROSITEPS01126; EF_TS_1; 1; .
PROSITEPS01127; EF_TS_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server