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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A910




SWISS-2DPAGE:  P0A910


P0A910


General information about the entry
View entry in simple text format
Entry nameOMPA_ECOLI
Primary accession numberP0A910
Secondary accession number(s) P02934
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Outer membrane protein A; AltName: Full=Outer membrane protein II*; Flags: Precursor;.
Gene nameName=ompA
Synonyms=con, tolG, tut
OrderedLocusNames=b0957, JW0940
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[4]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: MONOMER (PROBABLE)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.55; Mw=34492
pI=5.36; Mw=32758
pI=5.34; Mw=28591

MAPPING (identification):
GEL MATCHING [1] OR MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.23; Mw=14346  [identification data]
pI=5.25; Mw=13108  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [3].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.50; Mw=33875  [identification data]
pI=5.38; Mw=33875  [identification data]

EXPRESSION:
increase after benzoic acid treatment [4].

MAPPING (identification):
SPOT 2D-001WPP: Peptide mass fingerprinting [4];
SPOT 2D-001WPU: Tandem mass spectrometry [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP0A910; OMPA_ECOLI.
ECO2DBASEF033.1; 6TH EDITION.
ECO2DBASEF024.5; 6TH EDITION.
ECO2DBASEF028.0; 6TH EDITION.
ECO2DBASEF033.0; 6TH EDITION.
UniProtKB/Swiss-ProtP0A910; OMPA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameOMPA_ECOLI
Primary accession numberP0A910
Secondary accession number(s) P02934
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 69)
Name and origin of the protein
DescriptionRecName: Full=Outer membrane protein A; AltName: Full=Outer membrane protein II*; Flags: Precursor;
Gene nameName=ompA
Synonyms=con, tolG, tut
OrderedLocusNames=b0957, JW0940
Encoded onName=ompA; Synonyms=con, tolG, tut; OrderedLocusNames=b0957, JW0940
Keywords3D-structure; Cell membrane; Cell outer membrane; Complete proteome; Conjugation; Direct protein sequencing; Disulfide bond; Ion transport; Membrane; Porin; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane beta strand; Transport.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00307; CAA23588.1; -; Genomic_DNA
EMBLU00096; AAC74043.1; -; Genomic_DNA
EMBLAP009048; BAA35715.1; -; Genomic_DNA
PIRA93707; MMECA; .
RefSeqNP_415477.1; NC_000913.2; .
PDB1BXW; X-ray; 2.50 A; A=22-192
PDB1G90; NMR; -; A=22-197
PDB1QJP; X-ray; 1.65 A; A=22-192
PDB2GE4; NMR; -; A=22-197
PDB2JMM; NMR; -; A=23-197
PDB3NB3; EM; -; A/B/C=1-346
PDBsum1BXW; -; .
PDBsum1G90; -; .
PDBsum1QJP; -; .
PDBsum2GE4; -; .
PDBsum2JMM; -; .
PDBsum3NB3; -; .
ProteinModelPortalP0A910; -; .
SMRP0A910; 22-192; 208-337; .
DIPDIP-31879N; -; .
IntActP0A910; 10; .
MINTMINT-1308131; -; .
TCDB1.B.6.1.1; OmpA-OmpF porin (OOP) family; .
SWISS-2DPAGEP0A910; -; .
2DBase-EcoliP0A910; -; .
ECO2DBASEF024.5; 6TH EDITION; .
ECO2DBASEF028.0; 6TH EDITION; .
ECO2DBASEF033.0; 6TH EDITION; .
ECO2DBASEF033.1; 6TH EDITION; .
PRIDEP0A910; -; .
EnsemblBacteriaEBESCT00000002560; EBESCP00000002560; EBESCG00000002090; .
EnsemblBacteriaEBESCT00000002561; EBESCP00000002561; EBESCG00000002090; .
EnsemblBacteriaEBESCT00000014564; EBESCP00000013855; EBESCG00000013625; .
GeneID945571; -; .
GenomeReviewsAP009048_GR; JW0940; .
GenomeReviewsU00096_GR; b0957; .
KEGGecj:JW0940; -; .
KEGGeco:b0957; -; .
EchoBASEEB0663; -; .
EcoGeneEG10669; ompA; .
eggNOGCOG2885; -; .
GeneTreeEBGT00050000009918; -; .
HOGENOMHBG741096; -; .
OMADDNEAQK; -; .
ProtClustDBPRK10808; -; .
BioCycEcoCyc:EG10669-MONOMER; -; .
GenevestigatorP0A910; -; .
GOGO:0009279; C:cell outer membrane; IDA:EcoliWiki; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-KW; .
GOGO:0046930; C:pore complex; IEA:UniProtKB-KW; .
GOGO:0015288; F:porin activity; IDA:EcoCyc; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0005198; F:structural molecule activity; IEA:InterPro; .
GOGO:0000746; P:conjugation; IMP:EcoliWiki; .
GOGO:0009597; P:detection of virus; IMP:EcoliWiki; .
GOGO:0046718; P:entry of virus into host cell; IMP:EcoliWiki; .
GOGO:0006811; P:ion transport; IDA:EcoliWiki; .
InterProIPR011250; OMP_b-brl; .
InterProIPR006664; OMP_bac; .
InterProIPR002368; OmpA; .
InterProIPR006690; OMPA-like_CS; .
InterProIPR000498; OmpA-like_TM_dom; .
InterProIPR006665; OmpA/MotB_C; .
Gene3DG3DSA:2.40.160.20; OMP_b-brl; 1; .
Gene3DG3DSA:3.30.1330.60; OmpA/MotB_C; 1; .
PfamPF00691; OmpA; 1; .
PfamPF01389; OmpA_membrane; 1; .
PRINTSPR01021; OMPADOMAIN; .
PRINTSPR01022; OUTRMMBRANEA; .
SUPFAMSSF103088; OmpA/MotB_C; 1; .
PROSITEPS01068; OMPA_1; 1; .
PROSITEPS51123; OMPA_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server