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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0AFM2




SWISS-2DPAGE:  P0AFM2


P0AFM2


General information about the entry
View entry in simple text format
Entry namePROX_ECOLI
Primary accession numberP0AFM2
Secondary accession number(s) P14177
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=Glycine betaine-binding periplasmic protein; Flags: Precursor;.
Gene nameName=proX
Synonyms=proU
OrderedLocusNames=b2679, JW2654
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.65; Mw=33203  [identification data]
pI=5.44; Mw=33098  [identification data]

MAPPING (identification):
Tandem mass spectrometry [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP0AFM2; PROX_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePROX_ECOLI
Primary accession numberP0AFM2
Secondary accession number(s) P14177
Sequence was last modified on December 20, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 48)
Name and origin of the protein
DescriptionRecName: Full=Glycine betaine-binding periplasmic protein; Flags: Precursor;
Gene nameName=proX
Synonyms=proU
OrderedLocusNames=b2679, JW2654
Encoded onName=proX; Synonyms=proU; OrderedLocusNames=b2679, JW2654
Keywords3D-structure; Amino-acid transport; Complete proteome; Direct protein sequencing; Disulfide bond; Periplasm; Reference proteome; Signal; Transport.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM24856; AAA24429.1; -; Genomic_DNA
EMBLU00096; AAC75726.1; -; Genomic_DNA
EMBLAP009048; BAA16544.1; -; Genomic_DNA
PIRJS0130; BLECGP; .
RefSeqNP_417165.1; NC_000913.2; .
PDB1R9L; X-ray; 1.59 A; A=22-330
PDB1R9Q; X-ray; 2.05 A; A=22-330
PDBsum1R9L; -; .
PDBsum1R9Q; -; .
ProteinModelPortalP0AFM2; -; .
SMRP0AFM2; 22-330; .
IntActP0AFM2; 1; .
TCDB3.A.1.12.1; ATP-binding cassette (ABC) superfamily; .
SWISS-2DPAGEP0AFM2; -; .
EnsemblBacteriaEBESCT00000000020; EBESCP00000000020; EBESCG00000000020; .
EnsemblBacteriaEBESCT00000000021; EBESCP00000000021; EBESCG00000000020; .
EnsemblBacteriaEBESCT00000000022; EBESCP00000000022; EBESCG00000000020; .
EnsemblBacteriaEBESCT00000015581; EBESCP00000014872; EBESCG00000014641; .
GeneID947165; -; .
GenomeReviewsAP009048_GR; JW2654; .
GenomeReviewsU00096_GR; b2679; .
KEGGecj:JW2654; -; .
KEGGeco:b2679; -; .
EchoBASEEB0766; -; .
EcoGeneEG10773; proX; .
eggNOGCOG2113; -; .
GeneTreeEBGT00050000011397; -; .
HOGENOMHBG298949; -; .
OMANVAYTSI; -; .
ProtClustDBPRK11119; -; .
BioCycEcoCyc:PROX-MONOMER; -; .
GenevestigatorP0AFM2; -; .
GOGO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell; .
GOGO:0005488; F:binding; IEA:InterPro; .
GOGO:0005215; F:transporter activity; IEA:InterPro; .
GOGO:0006865; P:amino acid transport; IEA:UniProtKB-KW; .
InterProIPR007210; ABC_Gly_betaine_transp_sub-bd; .
PfamPF04069; OpuAC; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server