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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P10301




SWISS-2DPAGE:  P10301


P10301


General information about the entry
View entry in simple text format
Entry nameRRAS_HUMAN
Primary accession numberP10301
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onDecember 1, 2000 (version 2)
General Annotations were last modified on May 19, 2011 (version 9)
Name and origin of the protein
DescriptionRecName: Full=Ras-related protein R-Ras; AltName: Full=p23; Flags: Precursor;.
Gene nameName=RRAS
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=97295311; PubMed=9150953; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Wirth P., Jaccoud S., Appel R.D., Sarto C., Wilkins M.R., Hochstrasser D.F.
''''''Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots'';'';''
Electrophoresis 18(1):638-641(1997)
[2]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
2D PAGE maps for identified proteins
How to interpret a protein

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=4.79; Mw=21586
pI=4.87; Mw=21107
pI=4.91; Mw=20813

MAPPING (identification):
IMMUNOBLOTTING [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=4.81; Mw=21258
pI=4.88; Mw=20867
pI=4.93; Mw=20610

MAPPING (identification):
IMMUNOBLOTTING [1].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.88; Mw=19856
pI=4.94; Mw=19426
pI=4.99; Mw=19245

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP10301; RRAS_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameRRAS_HUMAN
Primary accession numberP10301
Secondary accession number(s) Q6FH12
Sequence was last modified on July 1, 1989 (version 1)
Annotations were last modified on October 19, 2011 (version 125)
Name and origin of the protein
DescriptionRecName: Full=Ras-related protein R-Ras; AltName: Full=p23; Flags: Precursor;
Gene nameName=RRAS
Encoded onName=RRAS
Keywords3D-structure; Cell membrane; Complete proteome; Direct protein sequencing; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM14949; AAA60256.1; -; Genomic_DNA
EMBLM14948; AAA60256.1; JOINED; Genomic_DNA
EMBLAF493920; AAM12634.1; -; mRNA
EMBLBT006805; AAP35451.1; -; mRNA
EMBLCR541944; CAG46742.1; -; mRNA
EMBLCR541967; CAG46765.1; -; mRNA
EMBLCH471177; EAW52506.1; -; Genomic_DNA
EMBLBC016286; AAH16286.1; -; mRNA
EMBLBC016318; AAH16318.1; -; mRNA
IPIIPI00020418; -; .
PIRA26159; TVHURR; .
RefSeqNP_006261.1; NM_006270.3; .
UniGeneHs.515536; -; .
PDB2FN4; X-ray; 1.65 A; A=23-201
PDBsum2FN4; -; .
ProteinModelPortalP10301; -; .
SMRP10301; 24-196; .
IntActP10301; 12; .
STRINGP10301; -; .
PhosphoSiteP10301; -; .
SWISS-2DPAGEP10301; -; .
PeptideAtlasP10301; -; .
PRIDEP10301; -; .
EnsemblENST00000246792; ENSP00000246792; ENSG00000126458; .
GeneID6237; -; .
KEGGhsa:6237; -; .
UCSCuc002pop.1; human; .
CTD6237; -; .
GeneCardsGC19M046515; -; .
H-InvDBHIX0015336; -; .
HGNCHGNC:10447; RRAS; .
HPACAB010160; -; .
MIM165090; gene; .
neXtProtNX_P10301; -; .
PharmGKBPA34861; -; .
eggNOGprNOG15226; -; .
GeneTreeENSGT00600000084306; -; .
HOGENOMHBG745225; -; .
HOVERGENHBG009351; -; .
InParanoidP10301; -; .
OMAFGASHHV; -; .
OrthoDBEOG4P5KB6; -; .
PhylomeDBP10301; -; .
Pathway_Interaction_DBephbfwdpathway; EPHB forward signaling; .
ReactomeREACT_13685; Synaptic Transmission; .
ReactomeREACT_18266; Axon guidance; .
NextBio24215; -; .
ArrayExpressP10301; -; .
BgeeP10301; -; .
CleanExHS_RRAS; -; .
GenevestigatorP10301; -; .
GermOnlineENSG00000126458; Homo sapiens; .
GOGO:0005886; C:plasma membrane; TAS:Reactome; .
GOGO:0019003; F:GDP binding; IDA:UniProtKB; .
GOGO:0005525; F:GTP binding; IEA:UniProtKB-KW; .
GOGO:0003924; F:GTPase activity; IDA:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:UniProtKB; .
GOGO:0007411; P:axon guidance; TAS:Reactome; .
GOGO:0007265; P:Ras protein signal transduction; TAS:ProtInc; .
GOGO:0007268; P:synaptic transmission; TAS:Reactome; .
InterProIPR005225; Small_GTP-bd_dom; .
InterProIPR001806; Small_GTPase; .
InterProIPR020849; Small_GTPase_Ras; .
PANTHERPTHR24070; PTHR24070; 1; .
PfamPF00071; Ras; 1; .
PRINTSPR00449; RASTRNSFRMNG; .
SMARTSM00173; RAS; 1; .
TIGRFAMsTIGR00231; Small_GTP; 1; .
PROSITEPS51421; RAS; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server