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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P20674




SWISS-2DPAGE:  P20674


P20674


General information about the entry
View entry in simple text format
Entry nameCOX5A_HUMAN
Primary accession numberP20674
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Cytochrome c oxidase subunit 5A, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide Va; Flags: Precursor;.
Gene nameName=COX5A
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.
''''''Human liver protein map: update 1993'';'';''
Electrophoresis 14(1):1216-1222(1993)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''''''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis'';'';''
Electrophoresis 16(1):1152-1159(1995)
[4]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[5]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[6]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[7]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[8]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[9]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
Comments
  • SUBUNIT: ONE OF THE NUCLEAR-CODED POLYPEPTIDE CHAINS OF CYTOCHROME C OXIDASE
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=4.80; Mw=12121

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [7].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=4.93; Mw=11055

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [8].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=4.82; Mw=9514

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=4.93; Mw=11646

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=4.90; Mw=11355

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [5][6].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=4.90; Mw=12400  [identification data]

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID COMPOSITION ANALYSIS [4].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.90; Mw=12293

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=4.75; Mw=16427  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [9].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=4.89; Mw=12353

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=4.91; Mw=12343

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP20674; P20674.
DOSAC-COBS-2DPAGEP20674; COX5A_HUMAN.
HSC-2DPAGEP20674; HUMAN.
OGP-WWWP20674.
Siena-2DPAGEP20674; COX5A_HUMAN.
UCD-2DPAGEP20674; COX5A_HUMAN.
UniProtKB/Swiss-ProtP20674; COX5A_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCOX5A_HUMAN
Primary accession numberP20674
Secondary accession number(s) P30045 Q8TB65
Sequence was last modified on December 16, 2008 (version 2)
Annotations were last modified on September 21, 2011 (version 104)
Name and origin of the protein
DescriptionRecName: Full=Cytochrome c oxidase subunit 5A, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide Va; Flags: Precursor;
Gene nameName=COX5A
Encoded onName=COX5A
KeywordsComplete proteome; Direct protein sequencing; Heme; Iron; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM22760; AAA99220.1; -; mRNA
EMBLDQ987236; ABK92283.1; -; mRNA
EMBLDQ987237; ABK92284.1; -; mRNA
EMBLCR407649; CAG28577.1; -; mRNA
EMBLAC125435; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLCH471136; EAW99288.1; -; Genomic_DNA
EMBLBC024240; AAH24240.1; -; mRNA
IPIIPI00025086; -; .
PIRJT0342; OTHU5A; .
RefSeqNP_004246.2; NM_004255.3; .
UniGeneHs.401903; -; .
ProteinModelPortalP20674; -; .
SMRP20674; 47-150; .
IntActP20674; 2; .
MINTMINT-1386385; -; .
STRINGP20674; -; .
TCDB3.D.4.11.1; proton-translocating cytochrome oxidase (COX) superfamily; .
PhosphoSiteP20674; -; .
SWISS-2DPAGEP20674; -; .
DOSAC-COBS-2DPAGEP20674; -; .
OGPP20674; -; .
Siena-2DPAGEP20674; -; .
UCD-2DPAGEP20674; -; .
PRIDEP20674; -; .
EnsemblENST00000322347; ENSP00000317780; ENSG00000178741; .
GeneID9377; -; .
KEGGhsa:9377; -; .
CTD9377; -; .
GeneCardsGC15M052009; -; .
H-InvDBHIX0202152; -; .
HGNCHGNC:2267; COX5A; .
HPAHPA027526; -; .
MIM603773; gene; .
neXtProtNX_P20674; -; .
PharmGKBPA26784; -; .
eggNOGprNOG20525; -; .
HOGENOMHBG381551; -; .
HOVERGENHBG051088; -; .
InParanoidP20674; -; .
OMAHHEETFE; -; .
OrthoDBEOG4D7Z71; -; .
PhylomeDBP20674; -; .
ReactomeREACT_6305; Respiratory electron transport; ATP synthesis by chemiosmotic coupling; and heat production by uncoupling proteins
ArrayExpressP20674; -; .
BgeeP20674; -; .
CleanExHS_COX5A; -; .
GenevestigatorP20674; -; .
GermOnlineENSG00000178741; Homo sapiens; .
GOGO:0005743; C:mitochondrial inner membrane; TAS:Reactome; .
GOGO:0004129; F:cytochrome-c oxidase activity; TAS:ProtInc; .
GOGO:0009055; F:electron carrier activity; TAS:UniProtKB; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0022904; P:respiratory electron transport chain; TAS:Reactome; .
InterProIPR003204; Cyt_c_oxidase_su5A; .
Gene3DG3DSA:1.25.40.40; Cyt_c_ox5A; 1; .
PANTHERPTHR14200; Cyt_c_ox5A; 1; .
PfamPF02284; COX5A; 1; .
SUPFAMSSF48479; Cyt_c_ox5A; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server