World-2DPAGE Home


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P23843




SWISS-2DPAGE:  P23843


P23843


General information about the entry
View entry in simple text format
Entry nameOPPA_ECOLI
Primary accession numberP23843
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 5)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Periplasmic oligopeptide-binding protein; Flags: Precursor;.
Gene nameName=oppA
OrderedLocusNames=b1243, JW1235
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.
'''''''98 Escherichia coli SWISS-2DPAGE database update'';'';''
Electrophoresis 19(1):1960-1971(1998)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[5]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.70; Mw=57529
pI=5.64; Mw=56854  [identification data]
pI=5.93; Mw=56188  [identification data]
pI=5.44; Mw=54555

MAPPING (identification):
SPOTS 2D-000KMQ, 2D-000KN0, 2D-000KOT: AMINO ACID COMPOSITION AND SEQUENCE TAG (ADVP) [3];
SPOT 2D-000KNL: AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI5.5-6.7 {Escherichia coli(5.5-6.7)}
Escherichia coli
ECOLI5.5-6.7
  map experimental info
  protein estimated location
 
ECOLI5.5-6.7

MAP LOCATIONS:
pI=5.65; Mw=50915

MAPPING (identification):
GEL MATCHING WITH ECOLI5-6 [4].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.13; Mw=50973  [identification data]
pI=5.47; Mw=50921  [identification data]
pI=5.63; Mw=50921  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.93; Mw=55635  [identification data]
pI=5.82; Mw=55131  [identification data]
pI=5.71; Mw=52681  [identification data]

EXPRESSION:
increase after benzoic acid treatment [5].

MAPPING (identification):
Peptide mass fingerprinting [5].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP23843; OPPA_ECOLI.
UniProtKB/Swiss-ProtP23843; OPPA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameOPPA_ECOLI
Primary accession numberP23843
Secondary accession number(s) P76829
Sequence was last modified on October 1, 1994 (version 2)
Annotations were last modified on October 19, 2011 (version 90)
Name and origin of the protein
DescriptionRecName: Full=Periplasmic oligopeptide-binding protein; Flags: Precursor;
Gene nameName=oppA
OrderedLocusNames=b1243, JW1235
Encoded onName=oppA; OrderedLocusNames=b1243, JW1235
KeywordsComplete proteome; Direct protein sequencing; Disulfide bond; Peptide transport; Periplasm; Protein transport; Reference proteome; Signal; Transport.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ05433; AAA21302.1; -; Genomic_DNA
EMBLM60918; AAB00918.1; -; Genomic_DNA
EMBLU00096; AAC74325.1; -; Genomic_DNA
EMBLAP009048; BAA14775.1; -; Genomic_DNA
EMBLX59501; CAA42089.1; -; Genomic_DNA
EMBLD83137; BAA11814.1; -; Genomic_DNA
PIRF64871; F64871; .
RefSeqNP_415759.1; NC_000913.2; .
ProteinModelPortalP23843; -; .
SMRP23843; 27-543; .
DIPDIP-10405N; -; .
IntActP23843; 1; .
SWISS-2DPAGEP23843; -; .
2DBase-EcoliP23843; -; .
EnsemblBacteriaEBESCT00000004482; EBESCP00000004482; EBESCG00000003658; .
EnsemblBacteriaEBESCT00000016524; EBESCP00000015815; EBESCG00000015584; .
GeneID945830; -; .
GenomeReviewsAP009048_GR; JW1235; .
GenomeReviewsU00096_GR; b1243; .
KEGGecj:JW1235; -; .
KEGGeco:b1243; -; .
EchoBASEEB0668; -; .
EcoGeneEG10674; oppA; .
eggNOGCOG4166; -; .
GeneTreeEBGT00070000031725; -; .
HOGENOMHBG688042; -; .
OMALANPNTA; -; .
ProtClustDBPRK15104; -; .
BioCycEcoCyc:OPPA-MONOMER; -; .
GenevestigatorP23843; -; .
GOGO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell; .
GOGO:0005215; F:transporter activity; IEA:InterPro; .
GOGO:0015833; P:peptide transport; IEA:UniProtKB-KW; .
GOGO:0015031; P:protein transport; IEA:UniProtKB-KW; .
InterProIPR000914; SBP_bac_5; .
InterProIPR023765; SBP_bac_5_CS; .
PfamPF00496; SBP_bac_5; 1; .
PROSITEPS01040; SBP_BACTERIAL_5; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server