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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P27797




SWISS-2DPAGE:  P27797


P27797


General information about the entry
View entry in simple text format
Entry nameCALR_HUMAN
Primary accession numberP27797
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onDecember 30, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 16)
Name and origin of the protein
DescriptionRecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor;.
Gene nameName=CALR
Synonyms=CRTC
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''''''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis'';'';''
Electrophoresis 16(1):1152-1159(1995)
[4]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[5]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[6]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[7]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[8]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[9]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
Comments
  • SUBUNIT: SINGLE CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=58700

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [7].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=58700

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [8].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=60638

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=4.36; Mw=59311
pI=4.40; Mw=58700
pI=4.42; Mw=58700

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=4.33; Mw=57239

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [5][6].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=58560  [identification data]

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID COMPOSITION ANALYSIS [4].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=58795

PATHOLOGICAL LEVEL:
INCREASED DURING HYPERCALCEMIA.

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=4.29; Mw=44500  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [9].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=59000

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=4.41; Mw=61232
pI=4.40; Mw=58200
pI=4.46; Mw=58200

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=3.73; Mw=58084

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP27797; P27797.
DOSAC-COBS-2DPAGEP27797; CALR_HUMAN.
HSC-2DPAGEP27797; HUMAN.
OGP-WWWP27797.
PHCI-2DPAGEP27797; CRTC_HUMAN.
PMMA-2DPAGEP27797; CRTC_HUMAN.
REPRODUCTION-2DPAGEIPI00020599; IPI00020599.
Siena-2DPAGEP27797; CALR_HUMAN.
UCD-2DPAGEP27797; CALR_HUMAN.
UniProtKB/Swiss-ProtP27797; CALR_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCALR_HUMAN
Primary accession numberP27797
Secondary accession number(s) Q6IAT4 Q9UDG2
Sequence was last modified on August 1, 1992 (version 1)
Annotations were last modified on October 19, 2011 (version 147)
Name and origin of the protein
DescriptionRecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor;
Gene nameName=CALR
Synonyms=CRTC
Encoded onName=CALR; Synonyms=CRTC
Keywords3D-structure; Acetylation; Calcium; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Extracellular matrix; Glycoprotein; Lectin; Metal-binding; Reference proteome; Repeat; Secreted; Signal; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM32294; AAA36582.1; -; mRNA
EMBLM84739; AAA51916.1; -; mRNA
EMBLAY047586; AAL13126.1; -; mRNA
EMBLAB451408; BAG70222.1; -; mRNA
EMBLBT007448; AAP36116.1; -; mRNA
EMBLCR457070; CAG33351.1; -; mRNA
EMBLAD000092; AAB51176.1; -; Genomic_DNA
EMBLCH471106; EAW84331.1; -; Genomic_DNA
EMBLBC002500; AAH02500.1; -; mRNA
EMBLBC007911; AAH07911.1; -; mRNA
EMBLBC020493; AAH20493.1; -; mRNA
IPIIPI00020599; -; .
PIRA42330; A37047; .
RefSeqNP_004334.1; NM_004343.3; .
UniGeneHs.515162; -; .
PDB2CLR; X-ray; 2.00 A; C/F=1-10
PDB3DOW; X-ray; 2.30 A; B=195-205
PDB3POS; X-ray; 1.65 A; A/B/C=18-368
PDB3POW; X-ray; 1.55 A; A=18-368
PDBsum2CLR; -; .
PDBsum3DOW; -; .
PDBsum3POS; -; .
PDBsum3POW; -; .
ProteinModelPortalP27797; -; .
SMRP27797; 13-362; .
DisProtDP00333; -; .
DIPDIP-104N; -; .
IntActP27797; 12; .
MINTMINT-101756; -; .
STRINGP27797; -; .
PhosphoSiteP27797; -; .
SWISS-2DPAGEP27797; -; .
Aarhus/Ghent-2DPAGE9401; IEF; .
DOSAC-COBS-2DPAGEP27797; -; .
OGPP27797; -; .
PHCI-2DPAGEP27797; -; .
PMMA-2DPAGEP27797; -; .
REPRODUCTION-2DPAGEIPI00020599; -; .
Siena-2DPAGEP27797; -; .
UCD-2DPAGEP27797; -; .
PRIDEP27797; -; .
EnsemblENST00000316448; ENSP00000320866; ENSG00000179218; .
GeneID811; -; .
KEGGhsa:811; -; .
UCSCuc002mvu.1; human; .
CTD811; -; .
GeneCardsGC19P012621; -; .
H-InvDBHIX0014813; -; .
HGNCHGNC:1455; CALR; .
HPACAB001513; -; .
HPACAB019952; -; .
HPAHPA002242; -; .
MIM109091; gene; .
neXtProtNX_P27797; -; .
PharmGKBPA26046; -; .
eggNOGprNOG13293; -; .
HOGENOMHBG444251; -; .
HOVERGENHBG005407; -; .
InParanoidP27797; -; .
OMADSNIYAY; -; .
OrthoDBEOG41JZCD; -; .
PhylomeDBP27797; -; .
ReactomeREACT_15380; Diabetes pathways; .
ReactomeREACT_17015; Metabolism of proteins; .
ReactomeREACT_6900; Immune System; .
DrugBankDB00009; Alteplase; .
DrugBankDB00029; Anistreplase; .
DrugBankDB00025; Antihemophilic Factor; .
DrugBankDB00015; Reteplase; .
DrugBankDB00031; Tenecteplase; .
NextBio3292; -; .
ArrayExpressP27797; -; .
BgeeP27797; -; .
CleanExHS_CALR; -; .
GenevestigatorP27797; -; .
GermOnlineENSG00000179218; Homo sapiens; .
GOGO:0009986; C:cell surface; TAS:BHF-UCL; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005788; C:endoplasmic reticulum lumen; IDA:UniProtKB; .
GOGO:0005615; C:extracellular space; IDA:BHF-UCL; .
GOGO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; TAS:Reactome; .
GOGO:0042824; C:MHC class I peptide loading complex; ISS:BHF-UCL; .
GOGO:0005634; C:nucleus; IDA:BHF-UCL; .
GOGO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL; .
GOGO:0005844; C:polysome; ISS:BHF-UCL; .
GOGO:0005578; C:proteinaceous extracellular matrix; IEA:UniProtKB-SubCell; .
GOGO:0050681; F:androgen receptor binding; IDA:BHF-UCL; .
GOGO:0005509; F:calcium ion binding; ISS:UniProtKB; .
GOGO:0051087; F:chaperone binding; TAS:BHF-UCL; .
GOGO:0001849; F:complement component C1q binding; TAS:BHF-UCL; .
GOGO:0003677; F:DNA binding; NAS:UniProtKB; .
GOGO:0005178; F:integrin binding; IPI:BHF-UCL; .
GOGO:0003729; F:mRNA binding; IDA:BHF-UCL; .
GOGO:0044183; F:protein binding involved in protein folding; TAS:BHF-UCL; .
GOGO:0005529; F:sugar binding; IEA:UniProtKB-KW; .
GOGO:0031625; F:ubiquitin protein ligase binding; IPI:UniProtKB; .
GOGO:0051082; F:unfolded protein binding; TAS:BHF-UCL; .
GOGO:0008270; F:zinc ion binding; TAS:BHF-UCL; .
GOGO:0007050; P:cell cycle arrest; IGI:BHF-UCL; .
GOGO:0090398; P:cellular senescence; IGI:BHF-UCL; .
GOGO:0042921; P:glucocorticoid receptor signaling pathway; TAS:BHF-UCL; .
GOGO:0045665; P:negative regulation of neuron differentiation; IDA:BHF-UCL; .
GOGO:0048387; P:negative regulation of retinoic acid receptor signaling pathway; IDA:BHF-UCL; .
GOGO:0033144; P:negative regulation of steroid hormone receptor signaling pathway; IDA:BHF-UCL; .
GOGO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL; .
GOGO:0017148; P:negative regulation of translation; ISS:BHF-UCL; .
GOGO:0002502; P:peptide antigen assembly with MHC class I protein complex; ISS:BHF-UCL; .
GOGO:0045787; P:positive regulation of cell cycle; IGI:BHF-UCL; .
GOGO:0008284; P:positive regulation of cell proliferation; IGI:BHF-UCL; .
GOGO:0045740; P:positive regulation of DNA replication; IGI:BHF-UCL; .
GOGO:0050766; P:positive regulation of phagocytosis; ISS:BHF-UCL; .
GOGO:0043687; P:post-translational protein modification; TAS:Reactome; .
GOGO:0006611; P:protein export from nucleus; IDA:UniProtKB; .
GOGO:0034504; P:protein localization to nucleus; IDA:UniProtKB; .
GOGO:0022417; P:protein maturation by protein folding; TAS:BHF-UCL; .
GOGO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome; .
GOGO:0050821; P:protein stabilization; ISS:UniProtKB; .
GOGO:0042981; P:regulation of apoptosis; TAS:UniProtKB; .
GOGO:0051208; P:sequestering of calcium ion; TAS:BHF-UCL; .
InterProIPR001580; Calret/calnex; .
InterProIPR018124; Calret/calnex_CS; .
InterProIPR009169; Calreticulin; .
InterProIPR008985; ConA-like_lec_gl; .
InterProIPR013320; ConA-like_subgrp; .
Gene3DG3DSA:2.60.120.200; ConA_like_subgrp; 2; .
PANTHERPTHR11073; Calret/calnex; 1; .
PfamPF00262; Calreticulin; 1; .
PIRSFPIRSF002356; Calreticulin; 1; .
PRINTSPR00626; CALRETICULIN; .
SUPFAMSSF49899; ConA_like_lec_gl; 2; .
PROSITEPS00803; CALRETICULIN_1; 1; .
PROSITEPS00804; CALRETICULIN_2; 1; .
PROSITEPS00805; CALRETICULIN_REPEAT; 3; .
PROSITEPS00014; ER_TARGET; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server