World-2DPAGE Home


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P28070




SWISS-2DPAGE:  P28070


P28070


General information about the entry
View entry in simple text format
Entry namePSB4_HUMAN
Primary accession numberP28070
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Proteasome subunit beta type-4; Short=Proteasome beta chain; EC=3.4.25.1; AltName: Full=26 kDa prosomal protein; Short=HsBPROS26; Short=PROS-26; AltName: Full=Macropain beta chain; AltName: Full=Multicatalytic endopeptidase complex beta chain; AltName: Full=Proteasome chain 3; Short=HsN3; Flags: Precursor;.
Gene nameName=PSMB4
Synonyms=PROS26
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.
''''''Human liver protein map: update 1993'';'';''
Electrophoresis 14(1):1216-1222(1993)
[2]   MAPPING ON GEL
MEDLINE=94147970; PubMed=8313871; [NCBI, ExPASy, EBI, Israel, Japan]
Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C., Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P., Hochstrasser D.F.
''''''Plasma and red blood cell protein maps: update 1993'';'';''
Electrophoresis 14(1):1223-1231(1993)
[3]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[4]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
Comments
  • SUBUNIT: THE PROTEASOME IS COMPOSED OF AT LEAST 12 NON IDENTICAL SUBUNITS WHICH FORM A HIGHLY ORDERED RING-SHAPED STRUCTURE
2D PAGE maps for identified proteins
How to interpret a protein

LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.50; Mw=24353

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=5.47; Mw=24400

MAPPING (identification):
GEL MATCHING WITH THE LYMPHOMA MASTER GEL [4].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=5.50; Mw=24300

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=5.50; Mw=24300

MAPPING (identification):
MICROSEQUENCING [2].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=5.50; Mw=24255

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP28070; P28070.
DOSAC-COBS-2DPAGEP28070; PSB4_HUMAN.
OGP-WWWP28070.
REPRODUCTION-2DPAGEIPI00555956; IPI00555956.
UniProtKB/Swiss-ProtP28070; PSB4_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePSB4_HUMAN
Primary accession numberP28070
Secondary accession number(s) B2R9L3 P31148 Q5SZS5 Q6IBI4 Q969L6
Sequence was last modified on October 17, 2006 (version 4)
Annotations were last modified on October 19, 2011 (version 134)
Name and origin of the protein
DescriptionRecName: Full=Proteasome subunit beta type-4; Short=Proteasome beta chain; EC=3.4.25.1; AltName: Full=26 kDa prosomal protein; Short=HsBPROS26; Short=PROS-26; AltName: Full=Macropain beta chain; AltName: Full=Multicatalytic endopeptidase complex beta chain; AltName: Full=Proteasome chain 3; Short=HsN3; Flags: Precursor;
Gene nameName=PSMB4
Synonyms=PROS26
Encoded onName=PSMB4; Synonyms=PROS26
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Hydrolase; Nucleus; Phosphoprotein; Polymorphism; Protease; Proteasome; Reference proteome; Threonine protease; Zymogen.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD26600; BAA05647.1; -; mRNA
EMBLAK313825; BAG36560.1; -; mRNA
EMBLCR456820; CAG33101.1; -; mRNA
EMBLBT006917; AAP35563.1; -; mRNA
EMBLAL589764; CAI16806.1; -; Genomic_DNA
EMBLCH471121; EAW53442.1; -; Genomic_DNA
EMBLS71381; AAB31085.1; -; mRNA
IPIIPI00555956; -; .
PIRS08186; S08186; .
PIRS45719; S45719; .
PIRS50147; S50147; .
RefSeqNP_002787.2; NM_002796.2; .
UniGeneHs.89545; -; .
ProteinModelPortalP28070; -; .
SMRP28070; 46-262; .
IntActP28070; 9; .
MINTMINT-1192686; -; .
STRINGP28070; -; .
MEROPST01.987; -; .
PhosphoSiteP28070; -; .
SWISS-2DPAGEP28070; -; .
Aarhus/Ghent-2DPAGE5104; IEF; .
DOSAC-COBS-2DPAGEP28070; -; .
OGPP28070; -; .
REPRODUCTION-2DPAGEIPI00555956; -; .
PeptideAtlasP28070; -; .
PRIDEP28070; -; .
EnsemblENST00000290541; ENSP00000290541; ENSG00000159377; .
GeneID5692; -; .
KEGGhsa:5692; -; .
UCSCuc001eyc.1; human; .
CTD5692; -; .
GeneCardsGC01P122750; -; .
H-InvDBHIX0001059; -; .
HGNCHGNC:9541; PSMB4; .
HPAHPA006700; -; .
MIM602177; gene; .
neXtProtNX_P28070; -; .
PharmGKBPA33886; -; .
eggNOGprNOG17937; -; .
GeneTreeENSGT00390000000698; -; .
HOGENOMHBG629728; -; .
HOVERGENHBG018194; -; .
InParanoidP28070; -; .
OMARIMRVND; -; .
OrthoDBEOG4F1X3R; -; .
PhylomeDBP28070; -; .
ReactomeREACT_11045; Signaling by Wnt; .
ReactomeREACT_13; Metabolism of amino acids and derivatives; .
ReactomeREACT_13505; Proteasome mediated degradation of PAK-2p34; .
ReactomeREACT_152; Cell Cycle; Mitotic; .
ReactomeREACT_1538; Cell Cycle Checkpoints; .
ReactomeREACT_21257; Metabolism of RNA; .
ReactomeREACT_383; DNA Replication; .
ReactomeREACT_578; Apoptosis; .
ReactomeREACT_6185; HIV Infection; .
ReactomeREACT_6850; Cdc20:Phospho-APC/C mediated degradation of Cyclin A; .
ReactomeREACT_6900; Immune System; .
NextBio22110; -; .
ArrayExpressP28070; -; .
BgeeP28070; -; .
CleanExHS_PSMB4; -; .
GenevestigatorP28070; -; .
GermOnlineENSG00000159377; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0005654; C:nucleoplasm; TAS:Reactome; .
GOGO:0005839; C:proteasome core complex; IEA:InterPro; .
GOGO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW; .
GOGO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome; .
GOGO:0002474; P:antigen processing and presentation of peptide antigen via MHC class I; TAS:Reactome; .
GOGO:0006915; P:apoptosis; TAS:Reactome; .
GOGO:0006977; P:DNA damage response; signal transduction by p53 class mediator resulting in cell cycle arrest; TAS:Reactome
GOGO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome; .
GOGO:0044419; P:interspecies interaction between organisms; IEA:UniProtKB-KW; .
GOGO:0000216; P:M/G1 transition of mitotic cell cycle; TAS:Reactome; .
GOGO:0016071; P:mRNA metabolic process; TAS:Reactome; .
GOGO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome; .
GOGO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome; .
GOGO:0000209; P:protein polyubiquitination; TAS:Reactome; .
GOGO:0042981; P:regulation of apoptosis; TAS:Reactome; .
GOGO:0006521; P:regulation of cellular amino acid metabolic process; TAS:Reactome; .
GOGO:0000084; P:S phase of mitotic cell cycle; TAS:Reactome; .
GOGO:0016032; P:viral reproduction; TAS:Reactome; .
InterProIPR016050; Proteasome_bsu_CS; .
InterProIPR016295; Proteasome_endopept_cplx_B; .
InterProIPR001353; Proteasome_sua/b; .
InterProIPR023333; Proteasome_suB-type; .
PfamPF00227; Proteasome; 1; .
PIRSFPIRSF001213; Psome_endopept_beta; 1; .
PROSITEPS00854; PROTEASOME_B_1; 1; .
PROSITEPS51476; PROTEASOME_B_2; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server