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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P38646




SWISS-2DPAGE:  P38646


P38646


General information about the entry
View entry in simple text format
Entry nameGRP75_HUMAN
Primary accession numberP38646
Secondary accession number(s) P30036
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 17)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; Short=MOT; AltName: Full=Peptide-binding protein 74; Short=PBP74; Flags: Precursor;.
Gene nameName=HSPA9
Synonyms=GRP75, HSPA9B
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.
''''''Human liver protein map: update 1993'';'';''
Electrophoresis 14(1):1216-1222(1993)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[4]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[5]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[6]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[7]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[8]   MAPPING ON GEL
PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=5.40; Mw=73173
pI=5.32; Mw=73173

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL AND MICROSEQUENCING [6].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=5.27; Mw=71362  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [7].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=5.33; Mw=24594

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=5.28; Mw=67250
pI=5.23; Mw=67250
pI=5.18; Mw=67055

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4][5].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=5.27; Mw=66791
pI=5.30; Mw=66571  [identification data]

MAPPING (identification):
MICROSEQUENCING AND AMINO ACID COMPOSITION ANALYSIS [3].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.30; Mw=25652

MAPPING (identification):
MICROSEQUENCING (ASEAIKGAV) [1].



NUCLEOLI_HELA_2D_HUMAN {2D-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_2D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_2D_HUMAN

MAP LOCATIONS:
pI=5.69; Mw=72414  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [8].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP38646; P38646.
DOSAC-COBS-2DPAGEP38646; GRP75_HUMAN.
HSC-2DPAGEP38646; HUMAN.
OGP-WWWP38646.
PHCI-2DPAGEP38646; GR75_HUMAN.
REPRODUCTION-2DPAGEIPI00007765; IPI00007765.
Siena-2DPAGEP38646; GRP75_HUMAN.
UCD-2DPAGEP38646; GRP75_HUMAN.
UniProtKB/Swiss-ProtP38646; GRP75_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameGRP75_HUMAN
Primary accession numberP38646
Secondary accession number(s) B2RCM1 P30036 P31932 Q1HB43 Q53H23 Q6GU03 Q9BWB7 Q9UC56
Sequence was last modified on May 27, 2002 (version 2)
Annotations were last modified on October 19, 2011 (version 126)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; Short=MOT; AltName: Full=Peptide-binding protein 74; Short=PBP74; Flags: Precursor;
Gene nameName=HSPA9
Synonyms=GRP75, HSPA9B
Encoded onName=HSPA9; Synonyms=GRP75, HSPA9B
Keywords3D-structure; Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL11066; -; NOT_ANNOTATED_CDS; mRNA
EMBLL15189; AAA67526.1; -; mRNA
EMBLAK315177; BAG37618.1; -; mRNA
EMBLAK222758; BAD96478.1; -; mRNA
EMBLDQ531046; ABF50973.1; -; Genomic_DNA
EMBLCH471062; EAW62129.1; -; Genomic_DNA
EMBLBC000478; AAH00478.1; -; mRNA
EMBLBC024034; AAH24034.1; -; mRNA
IPIIPI00007765; -; .
PIRB48127; B48127; .
RefSeqNP_004125.3; NM_004134.6; .
UniGeneHs.184233; -; .
PDB3N8E; X-ray; 2.80 A; A/B=439-597
PDBsum3N8E; -; .
ProteinModelPortalP38646; -; .
SMRP38646; 55-651; .
IntActP38646; 22; .
MINTMINT-1143092; -; .
STRINGP38646; -; .
PhosphoSiteP38646; -; .
SWISS-2DPAGEP38646; -; .
DOSAC-COBS-2DPAGEP38646; -; .
OGPP38646; -; .
PHCI-2DPAGEP38646; -; .
REPRODUCTION-2DPAGEIPI00007765; -; .
Siena-2DPAGEP38646; -; .
UCD-2DPAGEP38646; -; .
PRIDEP38646; -; .
EnsemblENST00000297185; ENSP00000297185; ENSG00000113013; .
GeneID3313; -; .
KEGGhsa:3313; -; .
UCSCuc003ldf.1; human; .
CTD3313; -; .
GeneCardsGC05M133080; -; .
H-InvDBHIX0005213; -; .
HGNCHGNC:5244; HSPA9; .
HPACAB005219; -; .
HPAHPA000898; -; .
MIM600548; gene; .
neXtProtNX_P38646; -; .
PharmGKBPA162391712; -; .
HOVERGENHBG051845; -; .
InParanoidP38646; -; .
OMAQAMYEAS; -; .
OrthoDBEOG49KFQ6; -; .
PhylomeDBP38646; -; .
NextBio13142; -; .
ArrayExpressP38646; -; .
BgeeP38646; -; .
GenevestigatorP38646; -; .
GermOnlineENSG00000113013; Homo sapiens; .
GOGO:0009986; C:cell surface; IDA:UniProtKB; .
GOGO:0042645; C:mitochondrial nucleoid; IDA:BHF-UCL; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; TAS:UniProtKB; .
GOGO:0006916; P:anti-apoptosis; TAS:UniProtKB; .
GOGO:0006457; P:protein folding; IEA:InterPro; .
InterProIPR012725; Chaperone_DnaK; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
TIGRFAMsTIGR02350; Prok_dnaK; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server