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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P60174




SWISS-2DPAGE:  P60174


P60174


General information about the entry
View entry in simple text format
Entry nameTPIS_HUMAN
Primary accession numberP60174
Secondary accession number(s) P00938
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase;.
Gene nameName=TPI1
Synonyms=TPI
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[4]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[5]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[6]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[7]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[8]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=6.38; Mw=25956

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [6].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=6.64; Mw=25260  [identification data]
pI=6.28; Mw=25260  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [7].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=6.84; Mw=24346

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=6.45; Mw=25716

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=6.55; Mw=23187
pI=6.84; Mw=23068

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4][5].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=6.75; Mw=23961  [identification data]
pI=6.84; Mw=24005

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID COMPOSITION ANALYSIS [3].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=6.46; Mw=25652
pI=6.79; Mw=25536

PATHOLOGICAL LEVEL:
ABSENT DURING CERTAIN CHRONIC HEMOLYTIC ANEMIA AND NEUROMUSCULAR DISORDERS.

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=7.38; Mw=25207  [identification data]
pI=7.24; Mw=25043  [identification data]
pI=7.00; Mw=25098  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [8].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=6.51; Mw=25638
pI=6.81; Mw=25475
pI=6.64; Mw=25420

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP60174; P60174.
DOSAC-COBS-2DPAGEP60174; TPIS_HUMAN.
HSC-2DPAGEP00938; HUMAN.
PMMA-2DPAGEP00938; TPIS_HUMAN.
REPRODUCTION-2DPAGEIPI00797687; IPI00797687.
REPRODUCTION-2DPAGEP60174; P60174.
Siena-2DPAGEP00938; TPIS_HUMAN.
UCD-2DPAGEP00938; P00938.
UCD-2DPAGEP60174; TPIS_HUMAN.
UniProtKB/Swiss-ProtP60174; TPIS_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTPIS_HUMAN
Primary accession numberP60174
Secondary accession number(s) D3DUS9 P00938 Q6FHP9 Q6IS07 Q8WWD0 Q96AG5
Sequence was last modified on October 19, 2011 (version 3)
Annotations were last modified on October 19, 2011 (version 97)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase;
Gene nameName=TPI1
Synonyms=TPI
Encoded onName=TPI1; Synonyms=TPI
Keywords3D-structure; Acetylation; Alternative promoter usage; Complete proteome; Direct protein sequencing; Disease mutation; Gluconeogenesis; Glycolysis; Isomerase; Pentose shunt; Phosphoprotein; Polymorphism; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM10036; AAB59511.1; ALT_INIT; mRNA
EMBLX69723; CAA49379.1; ALT_INIT; Genomic_DNA
EMBLU47924; AAB51316.1; ALT_INIT; Genomic_DNA
EMBLCH471116; EAW88722.1; -; Genomic_DNA
EMBLCH471116; EAW88723.1; -; Genomic_DNA
EMBLJ04603; AAN86636.1; ALT_FRAME; Genomic_DNA
EMBLBC007086; AAH07086.1; ALT_INIT; mRNA
EMBLBC007812; AAH07812.1; ALT_INIT; mRNA
EMBLBC009329; AAH09329.1; ALT_INIT; mRNA
EMBLBC011611; AAH11611.1; ALT_INIT; mRNA
EMBLBC015100; AAH15100.1; ALT_INIT; mRNA
EMBLBC017165; AAH17165.1; -; mRNA
EMBLBC017917; AAH17917.1; ALT_INIT; mRNA
EMBLBC070129; AAH70129.1; ALT_SEQ; mRNA
EMBLAK313282; BAG36090.1; ALT_INIT; mRNA
EMBLCR541702; CAG46503.1; -; mRNA
IPIIPI00451401; -; .
IPIIPI00797270; -; .
PIRS29743; ISHUT; .
RefSeqNP_000356.1; NM_000365.5; .
RefSeqNP_001152759.1; NM_001159287.1; .
UniGeneHs.524219; -; .
PDB1HTI; X-ray; 2.80 A; A/B=39-286
PDB1WYI; X-ray; 2.20 A; A/B=39-286
PDB2IAM; X-ray; 2.80 A; P=60-74
PDB2IAN; X-ray; 2.80 A; C/H/M/R=60-74
PDB2JK2; X-ray; 1.70 A; A/B=39-286
PDB2VOM; X-ray; 1.85 A; A/B/C/D=39-286
PDBsum1HTI; -; .
PDBsum1WYI; -; .
PDBsum2IAM; -; .
PDBsum2IAN; -; .
PDBsum2JK2; -; .
PDBsum2VOM; -; .
ProteinModelPortalP60174; -; .
SMRP60174; 4-249; .
IntActP60174; 6; .
MINTMINT-1384176; -; .
STRINGP60174; -; .
PhosphoSiteP60174; -; .
SWISS-2DPAGEP60174; -; .
Aarhus/Ghent-2DPAGE1111; IEF; .
Aarhus/Ghent-2DPAGE2112; IEF; .
DOSAC-COBS-2DPAGEP60174; -; .
PMMA-2DPAGEP60174; -; .
REPRODUCTION-2DPAGEIPI00797687; -; .
REPRODUCTION-2DPAGEP60174; -; .
Siena-2DPAGEP60174; -; .
UCD-2DPAGEP00938; -; .
UCD-2DPAGEP60174; -; .
PRIDEP60174; -; .
EnsemblENST00000229270; ENSP00000229270; ENSG00000111669; .
EnsemblENST00000396705; ENSP00000379933; ENSG00000111669; .
GeneID7167; -; .
KEGGhsa:7167; -; .
UCSCuc001qrk.1; human; .
CTD7167; -; .
GeneCardsGC12P006846; -; .
H-InvDBHIX0010385; -; .
HGNCHGNC:12009; TPI1; .
HPACAB004675; -; .
MIM190450; gene+phenotype; .
neXtProtNX_P60174; -; .
Orphanet868; Triose phosphate-isomerase deficiency; .
PharmGKBPA36689; -; .
eggNOGprNOG13381; -; .
HOGENOMHBG708281; -; .
HOVERGENHBG002599; -; .
InParanoidP60174; -; .
OrthoDBEOG40S0GF; -; .
PhylomeDBP60174; -; .
BioCycMetaCyc:HS03441-MONOMER; -; .
ReactomeREACT_474; Metabolism of carbohydrates; .
NextBio28066; -; .
ArrayExpressP60174; -; .
BgeeP60174; -; .
CleanExHS_TPI1; -; .
GenevestigatorP60174; -; .
GermOnlineENSG00000111669; Homo sapiens; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0004807; F:triose-phosphate isomerase activity; NAS:UniProtKB; .
GOGO:0006094; P:gluconeogenesis; TAS:Reactome; .
GOGO:0006096; P:glycolysis; TAS:Reactome; .
GOGO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-KW; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR022896; TrioseP_Isoase_bac/euk; .
InterProIPR000652; Triosephosphate_isomerase; .
InterProIPR020861; Triosephosphate_isomerase_AS; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PANTHERPTHR21139; Triophos_ismrse; 1; .
PfamPF00121; TIM; 1; .
SUPFAMSSF51351; Triophos_ismrse; 1; .
TIGRFAMsTIGR00419; Tim; 1; .
PROSITEPS00171; TIM_1; 1; .
PROSITEPS51440; TIM_2; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server