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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P60174 |
SWISS-2DPAGE: P60174
P60174:
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ID TPIS_HUMAN; STANDARD; 2DG.
AC P60174; P00938;
DT 01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT 31-MAR-2004, 2D annotation version 5.
DT 19-MAY-2011, general annotation version 12.
DE RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName:
DE Full=Triose-phosphate isomerase;.
GN Name=TPI1; Synonyms=TPI;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Hominidae; Homo.
OX NCBI_TaxID=9606;
MT CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
MT LIVER_HUMAN, LYMPHOCYTE_HUMAN, U937_HUMAN.
IM CEC_HUMAN, DLD1_HUMAN, ELC_HUMAN, HEPG2_HUMAN, HL60_HUMAN, KIDNEY_HUMAN,
IM LIVER_HUMAN, LYMPHOCYTE_HUMAN, U937_HUMAN.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=93162045; PubMed=1286669; [NCBI, Expasy, EBI, Israel, Japan]
RA Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F.,
RA Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel
RA R.D., Hughes G.J.;
RT ''''''Human liver protein map: a reference database established by
RT microsequencing and gel comparison'';'';'';
RL Electrophoresis 13(1):992-1001(1992).
RN [2]
RP MAPPING ON GEL.
RX MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA A., Hochstrasser D.F.;
RT ''''''Inside SWISS-2DPAGE database'';'';'';
RL Electrophoresis 16(1):1131-1151(1995).
RN [3]
RP MAPPING ON GEL.
RX MEDLINE=97295305; PubMed=9150947; [NCBI, Expasy, EBI, Israel, Japan]
RA Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA Mocarelli P.;
RT ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL Electrophoresis 18(1):599-604(1997).
RN [4]
RP MAPPING ON GEL.
RA Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL Submitted (JUL-1997) to SWISS-2DPAGE.
RN [5]
RP MAPPING ON GEL.
RX MEDLINE=96314065; PubMed=8740185; [NCBI, Expasy, EBI, Israel, Japan]
RA Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA Bladier D., Joubert-Caron R.;
RT ''''''Identification of different galectins by immunoblotting after two-
RT dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL Electrophoresis 17(1):600-606(1996).
RN [6]
RP MAPPING ON GEL.
RX MEDLINE=98163346; PubMed=9504818; [NCBI, Expasy, EBI, Israel, Japan]
RA Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT ''''''Standardized characterization of gene expression in human
RT colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL Electrophoresis 18(1):2842-2848(1997).
RN [7]
RP MAPPING ON GEL.
RA Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A.,
RA Appel R.D., Hochstrasser D.F.;
RL Submitted (JAN-1999) to SWISS-2DPAGE.
RN [8]
RP MAPPING ON GEL.
RA Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA P., Tissot J.-D.;
RT ''''''New insights into HIV lymphocyte infection'';'';'';
RL (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC -!- SUBUNIT: HOMODIMER
2D -!- MASTER: CEC_HUMAN;
2D -!- PI/MW: SPOT 2D-000UJS=6.38/25956;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [6].
2D -!- MASTER: DLD1_HUMAN;
2D -!- PI/MW: SPOT 2D-001ER3=6.64/25260;
2D -!- PI/MW: SPOT 2D-001ERC=6.28/25260;
2D -!- PEPTIDE MASSES: SPOT 2D-001ER3: 1414.79; 1458.76; 1466.72; 2192.04; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001ERC: 1137.46; 1234.54; 1414.72; 1458.64;
2D 1466.65; 1586.701; 1602.674; 1614.754; 1637.744; 2191.964; 3029.222; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [7].
2D -!- MASTER: ELC_HUMAN;
2D -!- PI/MW: SPOT 2D-000EPF=6.84/24346;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D -!- MASTER: HEPG2_HUMAN;
2D -!- PI/MW: SPOT 2D-0003MP=6.45/25716;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
2D -!- MASTER: HL60_HUMAN;
2D -!- PI/MW: SPOT 2D-000ZMG=6.55/23187;
2D -!- PI/MW: SPOT 2D-000ZMM=6.84/23068;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [4][5].
2D -!- MASTER: KIDNEY_HUMAN;
2D -!- PI/MW: SPOT 2D-000NRL=6.75/23961;
2D -!- PI/MW: SPOT 2D-000NRM=6.84/24005;
2D -!- AMINO ACID COMPOSITION: SPOT 2D-000NRL: B=12, Z=13.7, S=3.7, H=0.3,
2D G=11.8, T=5.2, A=11.3, P=3.5, Y=2.5, R=3.6, V=9.4, M=1.5, I=6.1, L=7.2,
2D F=2, K=6.3;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID
2D COMPOSITION ANALYSIS [3].
2D -!- MASTER: LIVER_HUMAN;
2D -!- PI/MW: SPOT 2D-0001C8=6.46/25652;
2D -!- PI/MW: SPOT 2D-0001CC=6.79/25536;
2D -!- PATHOLOGICAL LEVEL: ABSENT DURING CERTAIN CHRONIC HEMOLYTIC ANEMIA
2D AND NEUROMUSCULAR DISORDERS.
2D -!- MAPPING: MICROSEQUENCING [1].
2D -!- MASTER: LYMPHOCYTE_HUMAN;
2D -!- PI/MW: SPOT 2D-001YD7=7.38/25207;
2D -!- PI/MW: SPOT 2D-001YDB=7.24/25043;
2D -!- PI/MW: SPOT 2D-001YDC=7.00/25098;
2D -!- PEPTIDE MASSES: SPOT 2D-001YD7: 954.4686; 1082.5631; 1137.5636;
2D 1234.6166; 1326.6824; 1458.7152; 1466.7069; 1542.8403; 1586.7368;
2D 1602.776; 1614.8172; 1637.7974; 2192.061; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001YDB: 954.4686; 1082.5631; 1137.5636;
2D 1234.6166; 1326.6824; 1458.7152; 1466.7069; 1542.8403; 1586.7368;
2D 1614.8172; 2192.061; TRYPSIN.
2D -!- PEPTIDE MASSES: SPOT 2D-001YDC: 1082.5862; 1234.6576; 1458.7059;
2D 1466.7165; 1586.7503; 1614.8234; 2192.068; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [8].
2D -!- MASTER: U937_HUMAN;
2D -!- PI/MW: SPOT 2D-000D46=6.51/25638;
2D -!- PI/MW: SPOT 2D-000D48=6.81/25475;
2D -!- PI/MW: SPOT 2D-000D4A=6.64/25420;
2D -!- MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
CC ---------------------------------------------------------------------------
CC This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics.
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CC its content is in no way modified and this statement is not removed.
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CC ---------------------------------------------------------------------------
DR DOSAC-COBS 2D-PAGE; P60174; P60174.
DR DOSAC-COBS-2DPAGE; P60174; TPIS_HUMAN.
DR HSC-2DPAGE; P00938; HUMAN.
DR PMMA-2DPAGE; P00938; TPIS_HUMAN.
DR REPRODUCTION-2DPAGE; IPI00797687; IPI00797687.
DR REPRODUCTION-2DPAGE; P60174; P60174.
DR Siena-2DPAGE; P00938; TPIS_HUMAN.
DR UCD-2DPAGE; P00938; P00938.
DR UCD-2DPAGE; P60174; TPIS_HUMAN.
DR UniProtKB/Swiss-Prot; P60174; TPIS_HUMAN.
//
The following 2-D maps are available for this protein:
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Colorectal epithelia cells
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Colorectal adenocarcinoma cell line (DL-1)
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Erythroleukemia Cell
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HepG2
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Promyelocytic leukemia cells
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Kidney
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Liver
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Lymphocytes
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Macrophage Like Cell Line
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SWISS-2DPAGE (search AC)
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