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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P63284




SWISS-2DPAGE:  P63284


P63284


General information about the entry
View entry in simple text format
Entry nameCLPB_ECOLI
Primary accession numberP63284
Secondary accession number(s) P03815
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Chaperone protein ClpB; AltName: Full=Heat shock protein F84.1;.
Gene nameName=clpB
Synonyms=htpM
OrderedLocusNames=b2592, JW2573
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[5]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: THOUGHT TO BE AN ATPASE SUBUNIT OF AN INTRACELLULAR ATP-DEPENDENT PROTEASE
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.30; Mw=88421
pI=5.38; Mw=73900

EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [3].

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.44; Mw=34853  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.33; Mw=88745  [identification data]

EXPRESSION:
increase after benzoic acid treatment [5].

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [5].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
ECO2DBASEE072.0; 6TH EDITION.
ECO2DBASEF084.1; 6TH EDITION.
UniProtKB/Swiss-ProtP63284; CLPB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCLPB_ECOLI
Primary accession numberP63284
Secondary accession number(s) P03815
Sequence was last modified on October 11, 2004 (version 1)
Annotations were last modified on October 19, 2011 (version 76)
Name and origin of the protein
DescriptionRecName: Full=Chaperone protein ClpB; AltName: Full=Heat shock protein F84.1;
Gene nameName=clpB
Synonyms=htpM
OrderedLocusNames=b2592, JW2573
Encoded onName=clpB; Synonyms=htpM; OrderedLocusNames=b2592, JW2573
Keywords3D-structure; Acetylation; Alternative initiation; ATP-binding; Chaperone; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Reference proteome; Repeat; Stress response.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM29364; AAA24422.1; -; Genomic_DNA
EMBLU00096; AAC75641.1; -; Genomic_DNA
EMBLAP009048; BAA16476.1; -; Genomic_DNA
EMBLX57620; CAA40846.1; -; Genomic_DNA
EMBLV00350; CAA23639.1; -; Genomic_DNA
EMBLU50134; AAA92959.1; -; Genomic_DNA
PIRC65037; D35905; .
RefSeqNP_417083.1; NC_000913.2; .
PDB1JBK; X-ray; 1.80 A; A=159-351
PDB1KHY; X-ray; 1.95 A; A/B/C/D=1-148
PDBsum1JBK; -; .
PDBsum1KHY; -; .
ProteinModelPortalP63284; -; .
SMRP63284; 4-856; .
DIPDIP-35844N; -; .
IntActP63284; 72; .
MINTMINT-1222117; -; .
SWISS-2DPAGEP63284; -; .
ECO2DBASEE072.0; 6TH EDITION; .
ECO2DBASEF084.1; 6TH EDITION; .
PRIDEP63284; -; .
GeneID947077; -; .
GenomeReviewsAP009048_GR; JW2573; .
GenomeReviewsU00096_GR; b2592; .
KEGGecj:JW2573; -; .
KEGGeco:b2592; -; .
EchoBASEEB0155; -; .
EcoGeneEG10157; clpB; .
eggNOGCOG0542; -; .
GeneTreeEBGT00050000008751; -; .
HOGENOMHBG413133; -; .
OMADHAYIEP; -; .
ProtClustDBPRK10865; -; .
BioCycEcoCyc:EG10157-MONOMER; -; .
GenevestigatorP63284; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro; .
GOGO:0016485; P:protein processing; IEA:InterPro; .
GOGO:0009408; P:response to heat; IEA:InterPro; .
InterProIPR003593; ATPase_AAA+_core; .
InterProIPR013093; ATPase_AAA-2; .
InterProIPR003959; ATPase_AAA_core; .
InterProIPR018368; Chaperonin_ClpA/B_CS; .
InterProIPR017730; Chaperonin_ClpB; .
InterProIPR001270; Chaprnin_ClpA/B; .
InterProIPR019489; Clp_ATPase_C; .
InterProIPR004176; Clp_N; .
InterProIPR023150; Dbl_Clp-N; .
Gene3DG3DSA:1.10.1780.10; Dbl_Clp-N; 1; .
PfamPF00004; AAA; 1; .
PfamPF07724; AAA_2; 1; .
PfamPF02861; Clp_N; 2; .
PfamPF10431; ClpB_D2-small; 1; .
PRINTSPR00300; CLPPROTEASEA; .
SMARTSM00382; AAA; 2; .
TIGRFAMsTIGR03346; Chaperone_ClpB; 1; .
PROSITEPS00870; CLPAB_1; 1; .
PROSITEPS00871; CLPAB_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server