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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P63284 |
SWISS-2DPAGE: P63284
P63284:
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ID CLPB_ECOLI; STANDARD; 2DG.
AC P63284; P03815;
DT 01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT 15-MAY-2003, 2D annotation version 3.
DT 19-MAY-2011, general annotation version 14.
DE RecName: Full=Chaperone protein ClpB; AltName: Full=Heat shock protein F84.1;.
GN Name=clpB; Synonyms=htpM; OrderedLocusNames=b2592, JW2573;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
RA Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL Electrophoresis 17(1):547-555(1996).
RN [2]
RP MAPPING ON GEL.
RA Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL (IN) Neidhardt et al. (eds.);
RL Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN [3]
RP PROTEIN EXPRESSION.
RX MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
RA Gage D.J., Neidhardt F.C.;
RT ''''''Adaptation of Escherichia coli to the uncoupler of oxidative
RT phosphorylation 2,4-dinitrophenol'';'';'';
RL J. Bacteriol. 175(1):7105-7108(1993).
RN [4]
RP MAPPING ON GEL.
RX PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
RA Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA Sanchez J.-C.;
RT ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL Proteomics 1(1):409-423(2001).
RN [5]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
CC -!- SUBUNIT: THOUGHT TO BE AN ATPASE SUBUNIT OF AN INTRACELLULAR
CC ATP-DEPENDENT PROTEASE
2D -!- MASTER: ECOLI;
2D -!- PI/MW: SPOT 2D-000KEW=5.30/88421;
2D -!- PI/MW: SPOT 2D-000KI3=5.38/73900;
2D -!- EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [3].
2D -!- MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D -!- MASTER: ECOLI5-6;
2D -!- PI/MW: SPOT 2D-001LEJ=5.44/34853; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001LEJ: 915.399; 955.354; 971.372;
2D 1079.384; 1095.377; 1145.348; 1187.473; 1210.441; 1226.429; 1286.568;
2D 1560.698; 1655.694; 1732.586; 1748.565; 1921.823; 1989.81; 2005.79;
2D 2125.913; 2283.991; 2296.898; 2684.143; TRYPSIN.
2D -!- MAPPING: Peptide mass fingerprinting [4].
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WC2=5.33/88745;
2D -!- PEPTIDE MASSES: SPOT 2D-001WC2: 857.5; 956.48; 1010.47; 1057.55;
2D 1070.57; 1085.45; 1187.57; 1335.65; 1373.69; 1381.73; 1384.71; 1528.82;
2D 1560.84; 1748.76; 1758.93; 1774.93; 1955.03; 2006.04; 2048.13; 2124.11;
2D 2284.25; 2297.15; 2453.23; 2474.36; 3020.55; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WC2: (R)IINGEVPEGLKGR(R),223-235; (K)VFVAEPSVEDTIAILR(G),336-351.
2D -!- EXPRESSION: increase after benzoic acid treatment [5].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [5].
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DR ECO2DBASE; E072.0; 6TH EDITION.
DR ECO2DBASE; F084.1; 6TH EDITION.
DR UniProtKB/Swiss-Prot; P63284; CLPB_ECOLI.
//
The following 2-D maps are available for this protein:
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Escherichia coli
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Escherichia coli(5-6)
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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