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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P78527




SWISS-2DPAGE:  P78527


P78527


General information about the entry
View entry in simple text format
Entry namePRKDC_HUMAN
Primary accession numberP78527
integrated into SWISS-2DPAGE on May 15, 2003 (release 16)
2D Annotations were last modified onMarch 31, 2004 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=DNA-dependent protein kinase catalytic subunit; Short=DNA-PK catalytic subunit; Short=DNA-PKcs; EC=2.7.11.1; AltName: Full=DNPK1; AltName: Full=p460;.
Gene nameName=PRKDC
Synonyms=HYRC, HYRC1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
Comments
  • SUBUNIT: FORMS A COMPLEX WITH THE HETERODIMER KU70/KU80 AUTOANTIGEN. INTERACTS WITH DNA-PKCS INTERACTING PROTEIN (KIP) WITH THE REGION UPSTREAM THE KINASE DOMAIN
2D PAGE maps for identified proteins
How to interpret a protein

NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_1D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_1D_HUMAN

MAP LOCATIONS:
Mw=94000
Mw=94000  [identification data]
Mw=94000  [identification data]

MAPPING (identification):
Tandem mass spectrometry [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP78527; PRKDC_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePRKDC_HUMAN
Primary accession numberP78527
Secondary accession number(s) P78528 Q13327 Q13337 Q14175 Q59H99 Q7Z611 Q96SE6 Q9UME3
Sequence was last modified on October 31, 2003 (version 3)
Annotations were last modified on October 19, 2011 (version 131)
Name and origin of the protein
DescriptionRecName: Full=DNA-dependent protein kinase catalytic subunit; Short=DNA-PK catalytic subunit; Short=DNA-PKcs; EC=2.7.11.1; AltName: Full=DNPK1; AltName: Full=p460;
Gene nameName=PRKDC
Synonyms=HYRC, HYRC1
Encoded onName=PRKDC; Synonyms=HYRC, HYRC1
KeywordsAcetylation; Alternative splicing; ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; S-nitrosylation; Serine/threonine-protein kinase; TPR repeat; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU47077; AAB39925.5; -; mRNA
EMBLU34994; AAC50210.3; -; mRNA
EMBLAY316117; AAP69525.1; -; Genomic_DNA
EMBLU63630; AAC52019.2; -; Genomic_DNA
EMBLU90415; AAB51722.1; -; Genomic_DNA
EMBLL27425; AAA79244.1; -; Genomic_DNA
EMBLAB052953; BAB79635.1; -; Genomic_DNA
EMBLU35835; AAA79184.1; -; mRNA
EMBLAY030284; AAK40350.1; -; Genomic_DNA
EMBLAB208860; BAD92097.1; -; mRNA
IPIIPI00296337; -; .
IPIIPI00376215; -; .
PIRA57099; A57099; .
PIRG02083; G02083; .
RefSeqNP_001075109.1; NM_001081640.1; .
RefSeqNP_008835.5; NM_006904.6; .
UniGeneHs.491682; -; .
ProteinModelPortalP78527; -; .
SMRP78527; 3717-4006; 4097-4127; .
DIPDIP-24186N; -; .
IntActP78527; 38; .
MINTMINT-5006046; -; .
STRINGP78527; -; .
PhosphoSiteP78527; -; .
SWISS-2DPAGEP78527; -; .
PRIDEP78527; -; .
EnsemblENST00000314191; ENSP00000313420; ENSG00000121031; .
GeneID5591; -; .
KEGGhsa:5591; -; .
CTD5591; -; .
GeneCardsGC08M048685; -; .
GeneCardsGC08M048686; -; .
H-InvDBHIX0017562; -; .
HGNCHGNC:9413; PRKDC; .
HPACAB005167; -; .
MIM600899; gene; .
neXtProtNX_P78527; -; .
PharmGKBPA33776; -; .
eggNOGprNOG10627; -; .
GeneTreeENSGT00600000084475; -; .
HOVERGENHBG053681; -; .
InParanoidP78527; -; .
Pathway_Interaction_DBbard1pathway; BARD1 signaling events; .
Pathway_Interaction_DBpi3kciaktpathway; Class I PI3K signaling events mediated by Akt; .
Pathway_Interaction_DBar_pathway; Coregulation of Androgen receptor activity; .
Pathway_Interaction_DBfaspathway; FAS signaling pathway (CD95); .
ReactomeREACT_216; DNA Repair; .
NextBio21692; -; .
ArrayExpressP78527; -; .
BgeeP78527; -; .
GenevestigatorP78527; -; .
GermOnlineENSG00000121031; Homo sapiens; .
GOGO:0005958; C:DNA-dependent protein kinase-DNA ligase 4 complex; IDA:MGI; .
GOGO:0005667; C:transcription factor complex; IDA:BHF-UCL; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0003677; F:DNA binding; IEA:UniProtKB-KW; .
GOGO:0004677; F:DNA-dependent protein kinase activity; IDA:MGI; .
GOGO:0008134; F:transcription factor binding; IPI:BHF-UCL; .
GOGO:0032869; P:cellular response to insulin stimulus; IMP:BHF-UCL; .
GOGO:0006310; P:DNA recombination; IEA:UniProtKB-KW; .
GOGO:0006303; P:double-strand break repair via nonhomologous end joining; TAS:Reactome; .
GOGO:0018105; P:peptidyl-serine phosphorylation; IDA:BHF-UCL; .
GOGO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL; .
InterProIPR011989; ARM-like; .
InterProIPR016024; ARM-type_fold; .
InterProIPR003152; FATC; .
InterProIPR011009; Kinase-like_dom; .
InterProIPR012582; NUC194; .
InterProIPR000403; PI3/4_kinase_cat; .
InterProIPR018936; PI3/4_kinase_CS; .
InterProIPR003151; PIK-rel_kinase_FAT; .
InterProIPR014009; PIK_FAT; .
Gene3DG3DSA:1.25.10.10; ARM-like; 6; .
Gene3DG3DSA:1.10.1070.11; PI3/4_kinase_cat; 3; .
PfamPF02259; FAT; 1; .
PfamPF02260; FATC; 1; .
PfamPF08163; NUC194; 1; .
PfamPF00454; PI3_PI4_kinase; 1; .
SMARTSM00146; PI3Kc; 1; .
SUPFAMSSF48371; ARM-type_fold; 3; .
SUPFAMSSF56112; Kinase_like; 1; .
PROSITEPS51189; FAT; 1; .
PROSITEPS51190; FATC; 1; .
PROSITEPS50077; HEAT_REPEAT; FALSE_NEG; .
PROSITEPS00915; PI3_4_KINASE_1; 1; .
PROSITEPS00916; PI3_4_KINASE_2; 1; .
PROSITEPS50290; PI3_4_KINASE_3; 1; .
PROSITEPS50005; TPR; FALSE_NEG; .
PROSITEPS50293; TPR_REGION; FALSE_NEG; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server