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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P97457




SWISS-2DPAGE:  P97457


P97457


General information about the entry
View entry in simple text format
Entry nameMLRS_MOUSE
Primary accession numberP97457
integrated into SWISS-2DPAGE on September 1, 1998 (release 7)
2D Annotations were last modified onDecember 30, 2004 (version 2)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Myosin regulatory light chain 2, skeletal muscle isoform; AltName: Full=Fast skeletal myosin light chain 2; AltName: Full=MLC2F;.
Gene nameName=Mylpf
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   MAPPING ON GEL
MEDLINE=96089471; PubMed=8529625; [NCBI, ExPASy, EBI, Israel, Japan]
Corbett J.M., Wheeler C.H., Dunn M.J.
''''''Coelectrophoresis of cardiac tissue from human, dog, rat and mouse: towards the establishment of an integrated two-dimensional protein database'';'';''
Electrophoresis 16(1):1524-1529(1995)
[2]   MAPPING ON GEL
PubMed=11680894; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Chiappe D., Converset V., Hoogland C., Binz P.-A., Paesano S., Appel R.D., Wang S., Sennitt M., Nolan A., Cawthorne M.A., Hochstrasser D.F.
''''''The mouse SWISS-2DPAGE database: a tool for proteomics study of diabetes and obesity'';'';''
Proteomics 1(1):136-163(2001)
2D PAGE maps for identified proteins
How to interpret a protein

MUSCLE_MOUSE {Gastrocnemius muscle}
Mus musculus (Mouse)
Tissue: Gastrocnemius
MUSCLE_MOUSE
  map experimental info
  protein estimated location
 
MUSCLE_MOUSE

MAP LOCATIONS:
pI=4.68; Mw=17613  [identification data]
pI=4.78; Mw=17488  [identification data]
pI=4.68; Mw=15900  [identification data]
pI=4.78; Mw=15834  [identification data]
pI=4.68; Mw=14967  [identification data]
pI=4.77; Mw=14843  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [2]; SPOTS 2D-0017Q*, 2D-0017R*: MATCHING [2] WITH THE MOUSE CARDIAC 2-DE GEL [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UCD-2DPAGEP97457; MLRS_MOUSE.
UniProtKB/Swiss-ProtP97457; MLRS_MOUSE.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameMLRS_MOUSE
Primary accession numberP97457
Sequence was last modified on July 5, 2004 (version 3)
Annotations were last modified on September 21, 2011 (version 91)
Name and origin of the protein
DescriptionRecName: Full=Myosin regulatory light chain 2, skeletal muscle isoform; AltName: Full=Fast skeletal myosin light chain 2; AltName: Full=MLC2F;
Gene nameName=Mylpf
Encoded onName=Mylpf
KeywordsCalcium; Complete proteome; Methylation; Motor protein; Muscle protein; Myosin; Phosphoprotein; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU77943; AAB19118.1; -; mRNA
EMBLBC055869; AAH55869.1; -; mRNA
IPIIPI00224549; -; .
RefSeqNP_058034.1; NM_016754.5; .
UniGeneMm.14526; -; .
ProteinModelPortalP97457; -; .
SMRP97457; 17-166; .
IntActP97457; 1; .
STRINGP97457; -; .
PhosphoSiteP97457; -; .
SWISS-2DPAGEP97457; -; .
UCD-2DPAGEP97457; -; .
PRIDEP97457; -; .
EnsemblENSMUST00000032910; ENSMUSP00000032910; ENSMUSG00000030672; .
GeneID17907; -; .
KEGGmmu:17907; -; .
CTD29895; -; .
MGIMGI:97273; Mylpf; .
eggNOGroNOG04052; -; .
HOGENOMHBG746798; -; .
HOVERGENHBG012180; -; .
InParanoidP97457; -; .
OMAEEIRNMW; -; .
OrthoDBEOG4JT06P; -; .
PhylomeDBP97457; -; .
NextBio292737; -; .
ArrayExpressP97457; -; .
BgeeP97457; -; .
GenevestigatorP97457; -; .
GermOnlineENSMUSG00000030672; Mus musculus; .
GOGO:0016459; C:myosin complex; IEA:UniProtKB-KW; .
GOGO:0005509; F:calcium ion binding; IEA:InterPro; .
GOGO:0008307; F:structural constituent of muscle; IMP:BHF-UCL; .
GOGO:0007519; P:skeletal muscle tissue development; IMP:BHF-UCL; .
InterProIPR011992; EF-hand-like_dom; .
InterProIPR018247; EF_Hand_1_Ca_BS; .
InterProIPR018249; EF_HAND_2; .
InterProIPR002048; EF_hand_Ca-bd; .
Gene3DG3DSA:1.10.238.10; EF-Hand_type; 2; .
SMARTSM00054; EFh; 2; .
PROSITEPS00018; EF_HAND_1; 1; .
PROSITEPS50222; EF_HAND_2; 3; .



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