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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P99061




SWISS-2DPAGE:  P99061


P99061


General information about the entry
View entry in simple text format
Entry nameH33_SPISO
Primary accession numberP99061
integrated into SWISS-2DPAGE on November 9, 2001 (release 15)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 7)
Name and origin of the protein
DescriptionRecName: Full=Histone H3.3;.
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
2D PAGE maps for identified proteins
How to interpret a protein

NUCLEOLI_HELA_1D_HUMAN {SDS-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_1D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_1D_HUMAN

MAP LOCATIONS:
Mw=18078  [identification data]

MAPPING (identification):
Tandem mass spectrometry [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP84248; H33_SPISO.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameH33_SPISO
Primary accession numberP84248
Secondary accession number(s) P06351 P33155 Q9V3W4
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 5, 2010 (version 39)
Name and origin of the protein
DescriptionRecName: Full=Histone H3.3;
Gene nameRecName: Full=Histone H3.3
Encoded onRecName: Full=Histone H3.3
KeywordsAcetylation; Chromosome; DNA-binding; Methylation; Nucleosome core; Nucleus; Phosphoprotein.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM17876; AAA29965.1; -; mRNA
PIRA45941; A45941; .
ProteinModelPortalP84248; -; .
SMRP84248; 2-136; .
SWISS-2DPAGEP99061; -; .
GOGO:0000786; C:nucleosome; IEA:UniProtKB-KW; .
GOGO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GOGO:0003677; F:DNA binding; IEA:UniProtKB-KW; .
GOGO:0006334; P:nucleosome assembly; IEA:InterPro; .
InterProIPR009072; Histone-fold; .
InterProIPR007125; Histone_core_D; .
InterProIPR000164; Histone_H3; .
Gene3DG3DSA:1.10.20.10; Histone-fold; 1; .
PANTHERPTHR11426; Histone_H3; 1; .
PfamPF00125; Histone; 1; .
PRINTSPR00622; HISTONEH3; .
SMARTSM00428; H3; 1; .
SUPFAMSSF47113; Histone-fold; 1; .
PROSITEPS00322; HISTONE_H3_1; 1; .
PROSITEPS00959; HISTONE_H3_2; 1; .



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