World-2DPAGE Home


SWISS-2DPAGE 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PCNA_HUMAN




SWISS-2DPAGE:  PCNA_HUMAN


PCNA_HUMAN


General information about the entry
View entry in simple text format
Entry namePCNA_HUMAN
Primary accession numberP12004
integrated into SWISS-2DPAGE on September 1, 1997 (release 6)
2D Annotations were last modified onDecember 1, 2000 (version 1)
General Annotations were last modified on May 19, 2011 (version 10)
Name and origin of the protein
DescriptionRecName: Full=Proliferating cell nuclear antigen; Short=PCNA; AltName: Full=Cyclin;.
Gene nameName=PCNA
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=97295311; PubMed=9150953; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Wirth P., Jaccoud S., Appel R.D., Sarto C., Wilkins M.R., Hochstrasser D.F.
''''''Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots'';'';''
Electrophoresis 18(1):638-641(1997)
2D PAGE maps for identified proteins
How to interpret a protein

HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=4.62; Mw=35905

MAPPING (identification):
IMMUNOBLOTTING [1].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.75; Mw=37752

MAPPING (identification):
IMMUNOBLOTTING [1].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP12004; PCNA_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePCNA_HUMAN
Primary accession numberP12004
Secondary accession number(s) B2R897 D3DW02
Sequence was last modified on October 1, 1989 (version 1)
Annotations were last modified on October 19, 2011 (version 140)
Name and origin of the protein
DescriptionRecName: Full=Proliferating cell nuclear antigen; Short=PCNA; AltName: Full=Cyclin;
Gene nameName=PCNA
Encoded onName=PCNA
Keywords3D-structure; Acetylation; Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; Isopeptide bond; Nucleus; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM15796; AAA35736.1; -; mRNA
EMBLJ04718; AAA60040.1; -; Genomic_DNA
EMBLAF527838; AAM78556.1; -; Genomic_DNA
EMBLAK313286; BAG36094.1; -; mRNA
EMBLAL121924; CAC27344.1; -; Genomic_DNA
EMBLCH471133; EAX10428.1; -; Genomic_DNA
EMBLCH471133; EAX10429.1; -; Genomic_DNA
EMBLCH471133; EAX10430.1; -; Genomic_DNA
EMBLBC000491; AAH00491.1; -; mRNA
EMBLBC062439; AAH62439.1; -; mRNA
IPIIPI00021700; -; .
PIRA27445; WMHUET; .
RefSeqNP_002583.1; NM_002592.2; .
RefSeqNP_872590.1; NM_182649.1; .
UniGeneHs.147433; -; .
UniGeneHs.728886; -; .
PDB1AXC; X-ray; 2.60 A; A/C/E=1-261
PDB1U76; X-ray; 2.60 A; A/C/E=1-261
PDB1U7B; X-ray; 1.88 A; A=1-261
PDB1UL1; X-ray; 2.90 A; A/B/C=1-261
PDB1VYJ; X-ray; 2.80 A; A/C/E/G/I/K=1-261
PDB1VYM; X-ray; 2.30 A; A/B/C=1-261
PDB1W60; X-ray; 3.15 A; A/B=1-261
PDB2ZVK; X-ray; 2.70 A; A/B/C=1-261
PDB2ZVL; X-ray; 2.50 A; A/B/C/D/E/F=1-261
PDB2ZVM; X-ray; 2.30 A; A/B/C=1-261
PDB3P87; X-ray; 2.99 A; A/B/C/D/E/F=1-261
PDBsum1AXC; -; .
PDBsum1U76; -; .
PDBsum1U7B; -; .
PDBsum1UL1; -; .
PDBsum1VYJ; -; .
PDBsum1VYM; -; .
PDBsum1W60; -; .
PDBsum2ZVK; -; .
PDBsum2ZVL; -; .
PDBsum2ZVM; -; .
PDBsum3P87; -; .
ProteinModelPortalP12004; -; .
SMRP12004; 1-255; .
DIPDIP-1098N; -; .
IntActP12004; 18; .
MINTMINT-5000943; -; .
STRINGP12004; -; .
PhosphoSiteP12004; -; .
SWISS-2DPAGEP12004; -; .
PeptideAtlasP12004; -; .
PRIDEP12004; -; .
EnsemblENST00000379143; ENSP00000368438; ENSG00000132646; .
EnsemblENST00000379160; ENSP00000368458; ENSG00000132646; .
GeneID5111; -; .
KEGGhsa:5111; -; .
UCSCuc002wlp.1; human; .
CTD5111; -; .
GeneCardsGC20M005095; -; .
H-InvDBHIX0015618; -; .
HGNCHGNC:8729; PCNA; .
HPACAB000148; -; .
HPAHPA030521; -; .
HPAHPA030522; -; .
HPAHPA030523; -; .
MIM176740; gene; .
neXtProtNX_P12004; -; .
PharmGKBPA263; -; .
eggNOGprNOG19692; -; .
HOGENOMHBG749295; -; .
HOVERGENHBG000947; -; .
InParanoidP12004; -; .
OMAKAQDNAD; -; .
OrthoDBEOG4933JJ; -; .
PhylomeDBP12004; -; .
Pathway_Interaction_DBbard1pathway; BARD1 signaling events; .
ReactomeREACT_152; Cell Cycle; Mitotic; .
ReactomeREACT_216; DNA Repair; .
ReactomeREACT_22172; Chromosome Maintenance; .
ReactomeREACT_383; DNA Replication; .
NextBio19722; -; .
ArrayExpressP12004; -; .
BgeeP12004; -; .
CleanExHS_PCNA; -; .
GenevestigatorP12004; -; .
GermOnlineENSG00000132646; Homo sapiens; .
GOGO:0005737; C:cytoplasm; IDA:HPA; .
GOGO:0005663; C:DNA replication factor C complex; TAS:UniProtKB; .
GOGO:0015630; C:microtubule cytoskeleton; IDA:HPA; .
GOGO:0043596; C:nuclear replication fork; IDA:BHF-UCL; .
GOGO:0005654; C:nucleoplasm; TAS:Reactome; .
GOGO:0043626; C:PCNA complex; IEA:InterPro; .
GOGO:0070557; C:PCNA-p21 complex; IDA:UniProtKB; .
GOGO:0032139; F:dinucleotide insertion or deletion binding; IDA:BHF-UCL; .
GOGO:0030337; F:DNA polymerase processivity factor activity; IEA:InterPro; .
GOGO:0032405; F:MutLalpha complex binding; IDA:HGNC; .
GOGO:0000701; F:purine-specific mismatch base pair DNA N-glycosylase activity; IDA:BHF-UCL; .
GOGO:0008283; P:cell proliferation; TAS:ProtInc; .
GOGO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome; .
GOGO:0006298; P:mismatch repair; IDA:BHF-UCL; .
GOGO:0006297; P:nucleotide-excision repair; DNA gap filling; TAS:Reactome
GOGO:0048015; P:phosphatidylinositol-mediated signaling; NAS:UniProtKB; .
GOGO:0032077; P:positive regulation of deoxyribonuclease activity; IDA:UniProtKB; .
GOGO:0006275; P:regulation of DNA replication; IEA:InterPro; .
GOGO:0000083; P:regulation of transcription involved in G1/S phase of mitotic cell cycle; TAS:Reactome; .
GOGO:0000084; P:S phase of mitotic cell cycle; TAS:Reactome; .
GOGO:0000722; P:telomere maintenance via recombination; TAS:Reactome; .
GOGO:0032201; P:telomere maintenance via semi-conservative replication; TAS:Reactome; .
GOGO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome; .
GOGO:0019985; P:translesion synthesis; IDA:UniProtKB; .
InterProIPR000730; Pr_cel_nuc_antig; .
InterProIPR022649; Pr_cel_nuc_antig_C; .
InterProIPR022659; Pr_cel_nuc_antig_CS; .
InterProIPR022648; Pr_cel_nuc_antig_N; .
PANTHERPTHR11352; Pr_cel_nuc_antig; 1; .
PfamPF02747; PCNA_C; 1; .
PfamPF00705; PCNA_N; 1; .
PRINTSPR00339; PCNACYCLIN; .
TIGRFAMsTIGR00590; Pcna; 1; .
PROSITEPS01251; PCNA_1; 1; .
PROSITEPS00293; PCNA_2; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server