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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PDIA1_HUMAN




SWISS-2DPAGE:  PDIA1_HUMAN


PDIA1_HUMAN


General information about the entry
View entry in simple text format
Entry namePDIA1_HUMAN
Primary accession numberP07237
Secondary accession number(s) P30037 P32079
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor;.
Gene nameName=P4HB
Synonyms=ERBA2L, PDI, PDIA1, PO4DB
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=94147970; PubMed=8313871; [NCBI, ExPASy, EBI, Israel, Japan]
Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C., Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P., Hochstrasser D.F.
''''''Plasma and red blood cell protein maps: update 1993'';'';''
Electrophoresis 14(1):1223-1231(1993)
[4]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[5]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[6]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[7]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[8]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
2D PAGE maps for identified proteins
How to interpret a protein

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=4.77; Mw=54763
pI=4.80; Mw=54203  [identification data]
pI=4.84; Mw=54064

MAPPING (identification):
GEL MATCHING WITH HEPG2 MASTER GEL AND MASS FINGERPRINTING [7].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=4.76; Mw=57697
pI=4.80; Mw=57500

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=4.71; Mw=55308
pI=4.74; Mw=55308
pI=4.68; Mw=55462
pI=4.79; Mw=55308

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=4.73; Mw=56100

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [5][6].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=4.76; Mw=54532  [identification data]

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL AND AMINO ACID COMPOSITION ANALYSIS [4].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.76; Mw=54846
pI=4.82; Mw=54695

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=4.65; Mw=43614  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [8].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=4.74; Mw=54509

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=4.90; Mw=51909

MAPPING (identification):
MICROSEQUENCING [3].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=4.81; Mw=54949

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP07237; P07237.
DOSAC-COBS-2DPAGEP07237; PDIA1_HUMAN.
OGP-WWWP07237.
REPRODUCTION-2DPAGEIPI00010796; IPI00010796.
REPRODUCTION-2DPAGEP07237; P07237.
Siena-2DPAGEP07237; PDIA1_HUMAN.
UCD-2DPAGEP07237; PDIA1_HUMAN.
UniProtKB/Swiss-ProtP07237; PDIA1_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePDIA1_HUMAN
Primary accession numberP07237
Secondary accession number(s) B2RDQ2 P30037 P32079 Q15205 Q6LDE5
Sequence was last modified on November 1, 1997 (version 3)
Annotations were last modified on October 19, 2011 (version 163)
Name and origin of the protein
DescriptionRecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor;
Gene nameName=P4HB
Synonyms=ERBA2L, PDI, PDIA1, PO4DB
Encoded onName=P4HB; Synonyms=ERBA2L, PDI, PDIA1, PO4DB
Keywords3D-structure; Cell membrane; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Isomerase; Membrane; Redox-active center; Reference proteome; Repeat; Signal.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX05130; CAA28775.1; -; mRNA
EMBLJ02783; AAA61169.1; -; mRNA
EMBLM22806; AAC13652.1; -; Genomic_DNA
EMBLM22803; AAC13652.1; JOINED; Genomic_DNA
EMBLM22804; AAC13652.1; JOINED; Genomic_DNA
EMBLM22805; AAC13652.1; JOINED; Genomic_DNA
EMBLAK315631; BAG37999.1; -; mRNA
EMBLCH471099; EAW89690.1; -; Genomic_DNA
EMBLBC010859; AAH10859.1; -; mRNA
EMBLBC029617; AAH29617.1; -; mRNA
EMBLBC071892; AAH71892.1; -; mRNA
EMBLS37207; AAB22262.2; -; Genomic_DNA
EMBLX07077; CAA30112.1; -; mRNA
IPIIPI00010796; -; .
PIRA31913; ISHUSS; .
RefSeqNP_000909.2; NM_000918.3; .
UniGeneHs.464336; -; .
UniGeneHs.708779; -; .
PDB1BJX; NMR; -; A=136-245
PDB1MEK; NMR; -; A=18-137
PDB1X5C; NMR; -; A=368-475
PDB2BJX; NMR; -; A=136-245
PDB2K18; NMR; -; A=135-357
PDB3BJ5; X-ray; 2.20 A; A=230-368
PDBsum1BJX; -; .
PDBsum1MEK; -; .
PDBsum1X5C; -; .
PDBsum2BJX; -; .
PDBsum2K18; -; .
PDBsum3BJ5; -; .
ProteinModelPortalP07237; -; .
SMRP07237; 18-357; 368-471; .
IntActP07237; 15; .
MINTMINT-4999403; -; .
STRINGP07237; -; .
PhosphoSiteP07237; -; .
SWISS-2DPAGEP07237; -; .
Aarhus/Ghent-2DPAGE8412; IEF; .
Cornea-2DPAGEP07237; -; .
DOSAC-COBS-2DPAGEP07237; -; .
OGPP07237; -; .
REPRODUCTION-2DPAGEIPI00010796; -; .
REPRODUCTION-2DPAGEP07237; -; .
Siena-2DPAGEP07237; -; .
PeptideAtlasP07237; -; .
PRIDEP07237; -; .
EnsemblENST00000331483; ENSP00000327801; ENSG00000185624; .
GeneID5034; -; .
KEGGhsa:5034; -; .
NMPDRfig|9606.3.peg.14582; -; .
UCSCuc002kbn.1; human; .
CTD5034; -; .
GeneCardsGC17M075202; -; .
H-InvDBHIX0014256; -; .
HGNCHGNC:8548; P4HB; .
HPACAB012463; -; .
HPAHPA018884; -; .
MIM176790; gene; .
neXtProtNX_P07237; -; .
PharmGKBPA32876; -; .
eggNOGprNOG13821; -; .
GeneTreeENSGT00590000082936; -; .
HOGENOMHBG627841; -; .
HOVERGENHBG005920; -; .
InParanoidP07237; -; .
OMALMSQDLP; -; .
OrthoDBEOG4JWVDB; -; .
PhylomeDBP07237; -; .
BRENDA5.3.4.1; 2681; .
ReactomeREACT_22258; Metabolism of lipids and lipoproteins; .
NextBio19398; -; .
PMAP-CutDBP07237; -; .
ArrayExpressP07237; -; .
BgeeP07237; -; .
CleanExHS_P4HB; -; .
GenevestigatorP07237; -; .
GermOnlineENSG00000185624; Homo sapiens; .
GOGO:0009986; C:cell surface; IDA:UniProtKB; .
GOGO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome; .
GOGO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB; .
GOGO:0005576; C:extracellular region; NAS:UniProtKB; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0009055; F:electron carrier activity; IEA:InterPro; .
GOGO:0004656; F:procollagen-proline 4-dioxygenase activity; IDA:MGI; .
GOGO:0003756; F:protein disulfide isomerase activity; TAS:ProtInc; .
GOGO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro; .
GOGO:0045454; P:cell redox homeostasis; IEA:InterPro; .
GOGO:0006662; P:glycerol ether metabolic process; IEA:InterPro; .
GOGO:0006629; P:lipid metabolic process; TAS:Reactome; .
GOGO:0042157; P:lipoprotein metabolic process; TAS:Reactome; .
GOGO:0018401; P:peptidyl-proline hydroxylation to 4-hydroxy-L-proline; IDA:MGI; .
InterProIPR005788; Disulphide_isomerase; .
InterProIPR005792; Prot_disulphide_isomerase; .
InterProIPR005746; Thioredoxin; .
InterProIPR012336; Thioredoxin-like_fold; .
InterProIPR017937; Thioredoxin_CS; .
InterProIPR013766; Thioredoxin_domain; .
Gene3DG3DSA:3.40.30.10; Thioredoxin_fold; 3; .
PfamPF00085; Thioredoxin; 2; .
PRINTSPR00421; THIOREDOXIN; .
SUPFAMSSF52833; Thiordxn-like_fd; 4; .
TIGRFAMsTIGR01130; ER_PDI_fam; 1; .
TIGRFAMsTIGR01126; Pdi_dom; 2; .
PROSITEPS00014; ER_TARGET; 1; .
PROSITEPS00194; THIOREDOXIN_1; 2; .
PROSITEPS51352; THIOREDOXIN_2; 2; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server