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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: PDIA3_HUMAN




SWISS-2DPAGE:  PDIA3_HUMAN


PDIA3_HUMAN


General information about the entry
View entry in simple text format
Entry namePDIA3_HUMAN
Primary accession numberP30101
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 6)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor;.
Gene nameName=PDIA3
Synonyms=ERP57, ERP60, GRP58
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   SEQUENCE
MEDLINE=93162043; PubMed=1286667; [NCBI, ExPASy, EBI, Israel, Japan]
Rasmussen H.H., Van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
''''''Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes'';'';''
Electrophoresis 13(1):960-969(1992)
[3]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[4]   MAPPING ON GEL
MEDLINE=96007935; PubMed=7498158; [NCBI, ExPASy, EBI, Israel, Japan]
Gravel P., Sanchez J.-C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J.
''''''Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis'';'';''
Electrophoresis 16(1):1152-1159(1995)
[5]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[6]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[7]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[8]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[9]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[10]   MAPPING ON GEL
PubMed=12429849; [NCBI, ExPASy, EBI, Israel, Japan]
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
''''''Functional proteomic analysis of human nucleolus'';'';''
Mol. Biol. Cell. 13(1):4100-4109(2002)
[11]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=5.49; Mw=52805
pI=5.70; Mw=52483
pI=5.58; Mw=52697

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL AND MICROSEQUENCING [8].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=5.60; Mw=53100  [identification data]
pI=5.49; Mw=53373  [identification data]
pI=5.41; Mw=53511
pI=5.34; Mw=53236

MAPPING (identification):
SPOTS 2D-001DU*: Peptide mass fingerprinting [9]; SPOTS 2D-001DV*: MATCHING WITH THE HEPG2 MASTER GEL [9].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=5.46; Mw=56954
pI=5.53; Mw=56954
pI=5.61; Mw=56592

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=5.43; Mw=53036
pI=5.51; Mw=52740
pI=5.37; Mw=53036
pI=5.57; Mw=52740

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=5.57; Mw=53351
pI=5.68; Mw=52818

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [6][7].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=5.44; Mw=52322
pI=5.36; Mw=52082
pI=5.37; Mw=52202

MAPPING (identification):
MATCHING WITH LIVER AND U937 MASTERS [5].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.52; Mw=53499
pI=5.64; Mw=53351

MAPPING (identification):
MICROSEQUENCING (SDVLELTDD) [1][2].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=5.70; Mw=43221  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [11].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=5.64; Mw=52393

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



NUCLEOLI_HELA_2D_HUMAN {2D-PAGE of nucleolar proteins from Human HeLa cells}
Homo sapiens (Human)
Tissue: Cervix carcinoma
NUCLEOLI_HELA_2D_HUMAN
  map experimental info
  protein estimated location
 
NUCLEOLI_HELA_2D_HUMAN

MAP LOCATIONS:
pI=6.00; Mw=57865  [identification data]

MAPPING (identification):
Tandem mass spectrometry and peptide mass fingerprinting [10].



PLATELET_HUMAN {Platelet}
Homo sapiens (Human)
Tissue: Platelet
PLATELET_HUMAN
  map experimental info
  protein estimated location
 
PLATELET_HUMAN

MAP LOCATIONS:
pI=5.53; Mw=52296
pI=5.66; Mw=52002

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=5.54; Mw=51950

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP30101; P30101.
DOSAC-COBS-2DPAGEP30101; PDIA3_HUMAN.
OGP-WWWP30101.
PHCI-2DPAGEP30101; PDA3_HUMAN.
REPRODUCTION-2DPAGEP30101; P30101.
Siena-2DPAGEP30101; PDIA3_HUMAN.
UCD-2DPAGEP30101; PDIA3_HUMAN.
UniProtKB/Swiss-ProtP30101; PDIA3_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePDIA3_HUMAN
Primary accession numberP30101
Secondary accession number(s) Q13453 Q14255 Q8IYF8 Q9UMU7
Sequence was last modified on November 1, 1997 (version 4)
Annotations were last modified on October 19, 2011 (version 144)
Name and origin of the protein
DescriptionRecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor;
Gene nameName=PDIA3
Synonyms=ERP57, ERP60, GRP58
Encoded onName=PDIA3; Synonyms=ERP57, ERP60, GRP58
Keywords3D-structure; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Isomerase; Polymorphism; Redox-active center; Reference proteome; Repeat; Signal.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD16234; BAA03759.1; -; mRNA
EMBLU42068; AAC50331.1; -; mRNA
EMBLZ49835; CAA89996.1; -; mRNA
EMBLU75885; AAC51518.1; -; Genomic_DNA
EMBLU75875; AAC51518.1; JOINED; Genomic_DNA
EMBLU75876; AAC51518.1; JOINED; Genomic_DNA
EMBLU75877; AAC51518.1; JOINED; Genomic_DNA
EMBLU75878; AAC51518.1; JOINED; Genomic_DNA
EMBLU75879; AAC51518.1; JOINED; Genomic_DNA
EMBLU75880; AAC51518.1; JOINED; Genomic_DNA
EMBLU75881; AAC51518.1; JOINED; Genomic_DNA
EMBLU75882; AAC51518.1; JOINED; Genomic_DNA
EMBLU75883; AAC51518.1; JOINED; Genomic_DNA
EMBLU75884; AAC51518.1; JOINED; Genomic_DNA
EMBLD83485; BAA11928.1; -; mRNA
EMBLBC014433; AAH14433.1; -; mRNA
EMBLBC036000; AAH36000.4; -; mRNA
EMBLBC071878; AAH71878.1; -; mRNA
IPIIPI00025252; -; .
PIRJC5704; JC5704; .
PIRS55507; S55507; .
PIRS63994; S63994; .
PIRS68363; S68363; .
RefSeqNP_005304.3; NM_005313.4; .
UniGeneHs.591095; -; .
PDB2ALB; NMR; -; A=25-137
PDB2DMM; NMR; -; A=357-485
PDB2H8L; X-ray; 2.00 A; A/B/C=134-376
PDB3F8U; X-ray; 2.60 A; A/C=25-505
PDBsum2ALB; -; .
PDBsum2DMM; -; .
PDBsum2H8L; -; .
PDBsum3F8U; -; .
ProteinModelPortalP30101; -; .
SMRP30101; 25-493; .
DIPDIP-29132N; -; .
IntActP30101; 16; .
MINTMINT-5000005; -; .
STRINGP30101; -; .
PhosphoSiteP30101; -; .
SWISS-2DPAGEP30101; -; .
Aarhus/Ghent-2DPAGE5410; IEF; .
Cornea-2DPAGEP30101; -; .
DOSAC-COBS-2DPAGEP30101; -; .
PHCI-2DPAGEP30101; -; .
REPRODUCTION-2DPAGEP30101; -; .
Siena-2DPAGEP30101; -; .
UCD-2DPAGEP30101; -; .
PRIDEP30101; -; .
EnsemblENST00000300289; ENSP00000300289; ENSG00000167004; .
GeneID2923; -; .
KEGGhsa:2923; -; .
UCSCuc001zsu.1; human; .
CTD2923; -; .
GeneCardsGC15P020861; -; .
H-InvDBHIX0012190; -; .
H-InvDBHIX0028807; -; .
HGNCHGNC:4606; PDIA3; .
HPACAB011199; -; .
HPACAB015181; -; .
HPAHPA002645; -; .
HPAHPA003230; -; .
MIM602046; gene; .
neXtProtNX_P30101; -; .
PharmGKBPA29000; -; .
eggNOGprNOG15275; -; .
GeneTreeENSGT00590000082864; -; .
HOGENOMHBG627841; -; .
HOVERGENHBG005920; -; .
InParanoidP30101; -; .
OMAEGITLFR; -; .
OrthoDBEOG42Z4PX; -; .
PhylomeDBP30101; -; .
BRENDA5.3.4.1; 2681; .
ReactomeREACT_17015; Metabolism of proteins; .
ReactomeREACT_6900; Immune System; .
NextBio11593; -; .
ArrayExpressP30101; -; .
BgeeP30101; -; .
CleanExHS_PDIA3; -; .
GenevestigatorP30101; -; .
GermOnlineENSG00000167004; Homo sapiens; .
GOGO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0004197; F:cysteine-type endopeptidase activity; TAS:ProtInc; .
GOGO:0009055; F:electron carrier activity; IEA:InterPro; .
GOGO:0004629; F:phospholipase C activity; TAS:ProtInc; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0003756; F:protein disulfide isomerase activity; TAS:ProtInc; .
GOGO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro; .
GOGO:0002474; P:antigen processing and presentation of peptide antigen via MHC class I; TAS:Reactome; .
GOGO:0045454; P:cell redox homeostasis; IEA:InterPro; .
GOGO:0006662; P:glycerol ether metabolic process; IEA:InterPro; .
GOGO:0043687; P:post-translational protein modification; TAS:Reactome; .
GOGO:0006457; P:protein folding; TAS:Reactome; .
GOGO:0006606; P:protein import into nucleus; TAS:ProtInc; .
GOGO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome; .
GOGO:0006621; P:protein retention in ER lumen; TAS:ProtInc; .
GOGO:0007165; P:signal transduction; TAS:ProtInc; .
InterProIPR005788; Disulphide_isomerase; .
InterProIPR005792; Prot_disulphide_isomerase; .
InterProIPR005746; Thioredoxin; .
InterProIPR012336; Thioredoxin-like_fold; .
InterProIPR017937; Thioredoxin_CS; .
InterProIPR013766; Thioredoxin_domain; .
Gene3DG3DSA:3.40.30.10; Thioredoxin_fold; 3; .
PfamPF00085; Thioredoxin; 2; .
PRINTSPR00421; THIOREDOXIN; .
SUPFAMSSF52833; Thiordxn-like_fd; 4; .
TIGRFAMsTIGR01130; ER_PDI_fam; 1; .
TIGRFAMsTIGR01126; Pdi_dom; 2; .
PROSITEPS00194; THIOREDOXIN_1; 2; .
PROSITEPS51352; THIOREDOXIN_2; 2; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server