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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: POXB_ECOLI




SWISS-2DPAGE:  POXB_ECOLI


POXB_ECOLI


General information about the entry
View entry in simple text format
Entry namePOXB_ECOLI
Primary accession numberP07003
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onOctober 1, 2001 (version 1)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate dehydrogenase [ubiquinone]; EC=1.2.5.1; AltName: Full=Pyruvate oxidase; Short=POX; Contains: RecName: Full=Alpha-peptide;.
Gene nameName=poxB
OrderedLocusNames=b0871, JW0855
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
Comments
  • SUBUNIT: HOMOTERAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.86; Mw=56631

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
ECO2DBASEG058.0; 6TH EDITION.
UniProtKB/Swiss-ProtP07003; POXB_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePOXB_ECOLI
Primary accession numberP07003
Secondary accession number(s) Q47513 Q47514 Q47515 Q47516 Q47517 Q47518 Q47519 Q47520
Sequence was last modified on April 1, 1988 (version 1)
Annotations were last modified on October 19, 2011 (version 112)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate dehydrogenase [ubiquinone]; EC=1.2.5.1; AltName: Full=Pyruvate oxidase; Short=POX; Contains: RecName: Full=Alpha-peptide;
Gene nameName=poxB
OrderedLocusNames=b0871, JW0855
Encoded onName=poxB; OrderedLocusNames=b0871, JW0855
Keywords3D-structure; Cell membrane; Complete proteome; Direct protein sequencing; FAD; Flavoprotein; Lipid-binding; Magnesium; Membrane; Oxidoreductase; Reference proteome; Thiamine pyrophosphate.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX04105; CAA27725.1; -; Genomic_DNA
EMBLU00096; AAC73958.1; -; Genomic_DNA
EMBLAP009048; BAA35585.1; -; Genomic_DNA
EMBLS73268; AAB31180.1; -; Genomic_DNA
EMBLM28208; AAB59101.1; -; Genomic_DNA
EMBLL47688; AAB59102.1; -; Genomic_DNA
EMBLL47689; AAB59103.1; -; Genomic_DNA
EMBLL47690; AAB59104.1; -; Genomic_DNA
EMBLL47691; AAB59105.1; -; Genomic_DNA
EMBLL47692; AAB59106.1; -; Genomic_DNA
EMBLL47693; AAB59107.1; -; Genomic_DNA
EMBLL47694; AAB59108.1; -; Genomic_DNA
EMBLL47695; AAB59109.1; -; Genomic_DNA
PIRA23648; DEECPC; .
RefSeqNP_415392.1; NC_000913.2; .
PDB3EY9; X-ray; 2.90 A; A/B=1-572
PDB3EYA; X-ray; 2.50 A; A/B/C/D/E/F/G/H/I/J/K/L=1-549
PDBsum3EY9; -; .
PDBsum3EYA; -; .
ProteinModelPortalP07003; -; .
SMRP07003; 2-572; .
DIPDIP-36216N; -; .
IntActP07003; 3; .
SWISS-2DPAGEP07003; -; .
ECO2DBASEG058.0; 6TH EDITION; .
EnsemblBacteriaEBESCT00000000169; EBESCP00000000169; EBESCG00000000136; .
EnsemblBacteriaEBESCT00000016226; EBESCP00000015517; EBESCG00000015286; .
GeneID946132; -; .
GenomeReviewsAP009048_GR; JW0855; .
GenomeReviewsU00096_GR; b0871; .
KEGGecj:JW0855; -; .
KEGGeco:b0871; -; .
EchoBASEEB0747; -; .
EcoGeneEG10754; poxB; .
GeneTreeEBGT00050000008285; -; .
OMAIYGLVGD; -; .
ProtClustDBPRK09124; -; .
BioCycEcoCyc:PYRUVOXID-MONOMER; -; .
BioCycMetaCyc:PYRUVOXID-MONOMER; -; .
DrugBankDB00336; Nitrofurazone; .
GenevestigatorP07003; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0008289; F:lipid binding; IEA:UniProtKB-KW; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW; .
GOGO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro; .
GOGO:0016740; F:transferase activity; IEA:InterPro; .
InterProIPR012000; Thiamin_PyroP_enz_cen_dom; .
InterProIPR012001; Thiamin_PyroP_enz_TPP-bd_dom; .
InterProIPR000399; TPP-bd_CS; .
InterProIPR011766; TPP_enzyme-bd_C; .
PfamPF02775; TPP_enzyme_C; 1; .
PfamPF00205; TPP_enzyme_M; 1; .
PfamPF02776; TPP_enzyme_N; 1; .
PROSITEPS00187; TPP_ENZYMES; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server