Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

PRP19_HUMAN:

ID   PRP19_HUMAN; PRELIMINARY; 2DG.
AC   Q9UMS4;
DT   09-NOV-2001, integrated into SWISS-2DPAGE (release 15).
DT   30-DEC-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 7.
DE   RecName: Full=Pre-mRNA-processing factor 19; AltName: Full=Nuclear matrix
DE   protein 200; AltName: Full=PRP19/PSO4 homolog; Short=hPso4; AltName:
DE   Full=Senescence evasion factor;.
GN   Name=PRPF19; Synonyms=NMP200, PRP19, SNEV;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
IM   NUCLEOLI_HELA_1D_HUMAN, NUCLEOLI_HELA_2D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8H=56716;
1D   -!-   MW: BAND 1D-001V8I=55139;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8H: (K)TVPEELVKPEELSK(Y),193-206;
1D         (R)QVASHVGLHSASIPGILALDLCPSDTNK(I),209-236; (R)IWSVPNASCVQVVR(A),290-303; (K)TLQLDNNFEVK(S),429-439.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8I: (K)TVPEELVKPEELSK(Y),193-206.
1D   -!-   MAPPING: Tandem mass spectrometry [1].
2D   -!- MASTER: NUCLEOLI_HELA_2D_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001VKI=6.67/55941;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001VKI: 1374.8; 1798.8; 1917; 2093.3;
2D         2473.3; 2601.4; 2930.8; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
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DR   REPRODUCTION-2DPAGE; IPI00004968; IPI00004968.
DR   UniProtKB/Swiss-Prot; Q9UMS4; PRP19_HUMAN.
//

The following 2-D maps are available for this protein:

	SDS-PAGE of nucleolar proteins from Human HeLa cells	2D-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)