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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: SPTB1_HUMAN




SWISS-2DPAGE:  SPTB1_HUMAN


SPTB1_HUMAN


General information about the entry
View entry in simple text format
Entry nameSPTB1_HUMAN
Primary accession numberP11277
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMay 15, 2003 (version 3)
General Annotations were last modified on May 19, 2011 (version 15)
Name and origin of the protein
DescriptionRecName: Full=Spectrin beta chain, erythrocyte; AltName: Full=Beta-I spectrin;.
Gene nameName=SPTB
Synonyms=SPTB1
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=94147969; PubMed=8313870; [NCBI, ExPASy, EBI, Israel, Japan]
Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.
''''''Human liver protein map: update 1993'';'';''
Electrophoresis 14(1):1216-1222(1993)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=94147970; PubMed=8313871; [NCBI, ExPASy, EBI, Israel, Japan]
Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C., Sanchez J.-C., Tissot J.-D., Appel R.D., Walzer C., Balant L.P., Hochstrasser D.F.
''''''Plasma and red blood cell protein maps: update 1993'';'';''
Electrophoresis 14(1):1223-1231(1993)
[4]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[5]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
Comments
  • SUBUNIT: COMPOSED OF NONHOMOLOGOUS CHAINS, ALPHA AND BETA, WHICH AGGREGATE TO FORM DIMERS, TETRAMERS, AND HIGHER POLYMERS
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=5.60; Mw=25095

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=5.55; Mw=25144

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [5].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=5.56; Mw=25571

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=5.57; Mw=25536

MAPPING (identification):
MICROSEQUENCING [1].



RBC_HUMAN {Red blood cells}
Homo sapiens (Human)
Tissue: Erythrocyte
RBC_HUMAN
  map experimental info
  protein estimated location
 
RBC_HUMAN

MAP LOCATIONS:
pI=5.51; Mw=248963
pI=6.06; Mw=249959
pI=6.09; Mw=248963
pI=6.57; Mw=248963
pI=5.80; Mw=246984
pI=5.97; Mw=248963
pI=6.19; Mw=248963
pI=6.31; Mw=248963
pI=6.39; Mw=248963
pI=6.45; Mw=247972
pI=5.47; Mw=246000
pI=5.68; Mw=246984
pI=5.81; Mw=246000
pI=5.86; Mw=248963
pI=5.92; Mw=247972
pI=6.47; Mw=246984
pI=5.23; Mw=246000
pI=5.31; Mw=245020
pI=5.38; Mw=245020
pI=5.41; Mw=245020
pI=5.75; Mw=244044
pI=5.87; Mw=246000
pI=5.90; Mw=246000
pI=5.20; Mw=245020
pI=5.26; Mw=245020
pI=5.28; Mw=245020
pI=5.34; Mw=246000
pI=5.43; Mw=245020
pI=4.99; Mw=241139
pI=5.02; Mw=242104
pI=5.14; Mw=245020
pI=5.16; Mw=243072
pI=5.18; Mw=245020
pI=5.05; Mw=243072
pI=5.07; Mw=242104
pI=5.09; Mw=244044
pI=5.11; Mw=243072
pI=5.21; Mw=244044
pI=5.65; Mw=243072
pI=5.70; Mw=243072
pI=6.66; Mw=237320
pI=5.41; Mw=234495
pI=6.39; Mw=235433
pI=5.86; Mw=234495
pI=5.96; Mw=234495
pI=6.02; Mw=234495
pI=6.06; Mw=234495
pI=6.45; Mw=234495
pI=6.57; Mw=234495
pI=6.62; Mw=235433
pI=5.80; Mw=233561
pI=5.90; Mw=232631
pI=5.92; Mw=234495
pI=6.09; Mw=233561
pI=6.18; Mw=234495
pI=6.23; Mw=234495
pI=5.47; Mw=232631
pI=5.68; Mw=232631
pI=5.72; Mw=232631
pI=5.77; Mw=232631
pI=5.81; Mw=233561
pI=6.31; Mw=233561
pI=5.50; Mw=231704
pI=5.65; Mw=231704
pI=5.70; Mw=232631
pI=5.75; Mw=232631

MAPPING (identification):
COMIGRATION [3].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=5.52; Mw=25584

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
UniProtKB/Swiss-ProtP11277; SPTB1_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameSPTB1_HUMAN
Primary accession numberP11277
Secondary accession number(s) Q15510 Q15519
Sequence was last modified on November 25, 2008 (version 5)
Annotations were last modified on October 19, 2011 (version 132)
Name and origin of the protein
DescriptionRecName: Full=Spectrin beta chain, erythrocyte; AltName: Full=Beta-I spectrin;
Gene nameName=SPTB
Synonyms=SPTB1
Encoded onName=SPTB; Synonyms=SPTB1
Keywords3D-structure; Actin capping; Actin-binding; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disease mutation; Elliptocytosis; Hereditary hemolytic anemia; Phosphoprotein; Polymorphism; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ05500; AAA60578.1; -; mRNA
EMBLJ05500; AAA60579.1; -; mRNA
EMBLAL121774; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBLM37884; AAA63259.1; -; mRNA
EMBLM37885; AAA60571.1; -; mRNA
EMBLM57948; -; NOT_ANNOTATED_CDS; mRNA
EMBLX59510; CAA42097.1; -; mRNA
EMBLX59511; CAA42098.1; -; mRNA
EMBLM18054; AAA60572.1; -; mRNA
IPIIPI00216704; -; .
IPIIPI00237806; -; .
IPIIPI00783228; -; .
PIRA37064; SJHUB; .
RefSeqNP_000338.3; NM_000347.5; .
RefSeqNP_001020029.1; NM_001024858.2; .
UniGeneHs.417303; -; .
PDB1S35; X-ray; 2.40 A; A=1064-1275
PDB3EDU; X-ray; 2.10 A; A=1692-1907
PDB3F57; X-ray; 2.90 A; A/B=1686-1907
PDB3KBT; X-ray; 2.75 A; A/B=1583-1906
PDB3KBU; X-ray; 2.75 A; A/B=1583-1906
PDB3LBX; X-ray; 2.80 A; B=1902-2084
PDBsum1S35; -; .
PDBsum3EDU; -; .
PDBsum3F57; -; .
PDBsum3KBT; -; .
PDBsum3KBU; -; .
PDBsum3LBX; -; .
ProteinModelPortalP11277; -; .
SMRP11277; 49-282; 297-1273; 1305-1367
1380-2083.
DIPDIP-1021N; -; .
IntActP11277; 3; .
STRINGP11277; -; .
PhosphoSiteP11277; -; .
SWISS-2DPAGEP11277; -; .
PRIDEP11277; -; .
EnsemblENST00000389721; ENSP00000374371; ENSG00000070182; .
GeneID6710; -; .
KEGGhsa:6710; -; .
CTD6710; -; .
GeneCardsGC14M045384; -; .
HGNCHGNC:11274; SPTB; .
HPACAB015169; -; .
HPAHPA003394; -; .
HPAHPA003398; -; .
MIM130600; phenotype; .
MIM182870; gene+phenotype; .
neXtProtNX_P11277; -; .
Orphanet822; Hereditary spherocytosis; .
PharmGKBPA36103; -; .
HOVERGENHBG057912; -; .
ReactomeREACT_18266; Axon guidance; .
ReactomeREACT_92778; Axon guidance; .
ArrayExpressP11277; -; .
BgeeP11277; -; .
CleanExHS_SPTB; -; .
GenevestigatorP11277; -; .
GermOnlineENSG00000070182; Homo sapiens; .
GOGO:0009986; C:cell surface; IDA:UniProtKB; .
GOGO:0005829; C:cytosol; TAS:Reactome; .
GOGO:0031235; C:intrinsic to internal side of plasma membrane; TAS:BHF-UCL; .
GOGO:0043234; C:protein complex; IDA:UniProtKB; .
GOGO:0008091; C:spectrin; TAS:ProtInc; .
GOGO:0014731; C:spectrin-associated cytoskeleton; IDA:BHF-UCL; .
GOGO:0051015; F:actin filament binding; IDA:UniProtKB; .
GOGO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro; .
GOGO:0051693; P:actin filament capping; IEA:UniProtKB-KW; .
GOGO:0007411; P:axon guidance; TAS:Reactome; .
InterProIPR001589; Actinin_actin-bd_CS; .
InterProIPR001715; CH-domain; .
InterProIPR018159; Spectrin/alpha-actinin; .
InterProIPR016343; Spectrin_bsu; .
InterProIPR002017; Spectrin_repeat; .
Gene3DG3DSA:1.10.418.10; Calponin-homology; 2; .
PfamPF00307; CH; 2; .
PfamPF00435; Spectrin; 17; .
PIRSFPIRSF002297; Spectrin_beta_subunit; 1; .
SMARTSM00033; CH; 2; .
SMARTSM00150; SPEC; 17; .
SUPFAMSSF47576; Calponin-homology; 1; .
PROSITEPS00019; ACTININ_1; 1; .
PROSITEPS00020; ACTININ_2; 1; .
PROSITEPS50021; CH; 2; .



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