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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: SYS_ECOLI




SWISS-2DPAGE:  SYS_ECOLI


SYS_ECOLI


General information about the entry
View entry in simple text format
Entry nameSYS_ECOLI
Primary accession numberP0A8L1
Secondary accession number(s) P09156
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 1)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Seryl-tRNA synthetase; EC=6.1.1.11; AltName: Full=Serine--tRNA ligase; Short=SerRS; AltName: Full=Seryl-tRNA(Ser/Sec) synthetase;.
Gene nameName=serS
OrderedLocusNames=b0893, JW0876
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
Comments
  • SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.34; Mw=51128

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP0A8L1; SYS_ECOLI.
ECO2DBASEE048.8; 6TH EDITION.
UniProtKB/Swiss-ProtP0A8L1; SYS_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameSYS_ECOLI
Primary accession numberP0A8L1
Secondary accession number(s) P09156
Sequence was last modified on June 7, 2005 (version 1)
Annotations were last modified on October 19, 2011 (version 61)
Name and origin of the protein
DescriptionRecName: Full=Seryl-tRNA synthetase; EC=6.1.1.11; AltName: Full=Serine--tRNA ligase; Short=SerRS; AltName: Full=Seryl-tRNA(Ser/Sec) synthetase;
Gene nameName=serS
OrderedLocusNames=b0893, JW0876
Encoded onName=serS; OrderedLocusNames=b0893, JW0876
KeywordsAminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX05017; CAA28673.1; -; Genomic_DNA
EMBLU00096; AAC73979.1; -; Genomic_DNA
EMBLAP009048; BAA35625.1; -; Genomic_DNA
EMBLJ03412; AAA83842.1; -; Genomic_DNA
PIRA26400; YSEC; .
RefSeqNP_415413.1; NC_000913.2; .
ProteinModelPortalP0A8L1; -; .
SMRP0A8L1; 1-430; .
DIPDIP-35989N; -; .
IntActP0A8L1; 16; .
MINTMINT-1230011; -; .
SWISS-2DPAGEP0A8L1; -; .
2DBase-EcoliP0A8L1; -; .
ECO2DBASEE048.8; 6TH EDITION; .
EnsemblBacteriaEBESCT00000001063; EBESCP00000001063; EBESCG00000000879; .
EnsemblBacteriaEBESCT00000015262; EBESCP00000014553; EBESCG00000014322; .
GeneID945506; -; .
GenomeReviewsAP009048_GR; JW0876; .
GenomeReviewsU00096_GR; b0893; .
KEGGecj:JW0876; -; .
KEGGeco:b0893; -; .
EchoBASEEB0940; -; .
EcoGeneEG10947; serS; .
eggNOGCOG0172; -; .
GeneTreeEBGT00050000009989; -; .
HOGENOMHBG629391; -; .
OMAHTKPLEE; -; .
ProtClustDBPRK05431; -; .
BioCycEcoCyc:SERS-MONOMER; -; .
BioCycMetaCyc:SERS-MONOMER; -; .
GenevestigatorP0A8L1; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0000287; F:magnesium ion binding; IDA:EcoCyc; .
GOGO:0004828; F:serine-tRNA ligase activity; IDA:UniProtKB; .
GOGO:0016260; P:selenocysteine biosynthetic process; IDA:EcoCyc; .
GOGO:0006434; P:seryl-tRNA aminoacylation; IDA:UniProtKB; .
HAMAPMF_00176; Ser_tRNA_synth_type1; 1; -
InterProIPR002314; aa-tRNA-synt_IIb_cons-dom; .
InterProIPR006195; aa-tRNA-synth_II; .
InterProIPR002317; Ser-tRNA-synth_IIa; .
InterProIPR015866; Ser-tRNA-synth_IIa_N; .
InterProIPR010978; tRNA-bd_arm; .
Gene3DG3DSA:1.10.287.40; Ser-tRNA-synth_IIa_N; 1; .
PANTHERPTHR11778; tRNA-synt_ser; 1; .
PfamPF02403; Seryl_tRNA_N; 1; .
PfamPF00587; tRNA-synt_2b; 1; .
PIRSFPIRSF001529; Ser-tRNA-synth_IIa; 1; .
PRINTSPR00981; TRNASYNTHSER; .
SUPFAMSSF46589; tRNA_binding_arm; 1; .
TIGRFAMsTIGR00414; SerS; 1; .
PROSITEPS50862; AA_TRNA_LIGASE_II; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server