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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: TRPA_ECOLI




SWISS-2DPAGE:  TRPA_ECOLI


TRPA_ECOLI


General information about the entry
View entry in simple text format
Entry nameTRPA_ECOLI
Primary accession numberP0A877
Secondary accession number(s) P00928
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 4)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Tryptophan synthase alpha chain; EC=4.2.1.20;.
Gene nameName=trpA
OrderedLocusNames=b1260, JW1252
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
MEDLINE=80159887; PubMed=6988414; [NCBI, ExPASy, EBI, Israel, Japan]
Bloch P.L., Phillips T.A., Neidhardt F.C.
''''''Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins'';'';''
J. Bacteriol. 141(1):1409-1420(1980)
[4]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
Comments
  • SUBUNIT: TETRAMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.30; Mw=28724

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2] AND BLOCH [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.38; Mw=28936  [identification data]
pI=5.37; Mw=27906  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.18; Mw=32691  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [4].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP0A877; TRPA_ECOLI.
ECO2DBASEE025.5; 6TH EDITION.
UniProtKB/Swiss-ProtP0A877; TRPA_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameTRPA_ECOLI
Primary accession numberP0A877
Secondary accession number(s) P00928 Q47669
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 57)
Name and origin of the protein
DescriptionRecName: Full=Tryptophan synthase alpha chain; EC=4.2.1.20;
Gene nameName=trpA
OrderedLocusNames=b1260, JW1252
Encoded onName=trpA; OrderedLocusNames=b1260, JW1252
Keywords3D-structure; Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Complete proteome; Direct protein sequencing; Lyase; Reference proteome; Tryptophan biosynthesis.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV00364; CAA23662.1; -; Genomic_DNA
EMBLV00372; CAA23675.1; -; Genomic_DNA
EMBLJ01714; AAA57301.1; -; Genomic_DNA
EMBLU23489; AAB60035.1; -; Genomic_DNA
EMBLU23490; AAA65140.1; -; Genomic_DNA
EMBLU23491; AAA65146.1; -; Genomic_DNA
EMBLU23492; AAA65152.1; -; Genomic_DNA
EMBLU23494; AAA65164.1; -; Genomic_DNA
EMBLU25417; AAA73791.1; -; Genomic_DNA
EMBLU25418; AAA73797.1; -; Genomic_DNA
EMBLU25419; AAA73803.1; -; Genomic_DNA
EMBLU25420; AAA73809.1; -; Genomic_DNA
EMBLU25421; AAA73815.1; -; Genomic_DNA
EMBLU25422; AAA73821.1; -; Genomic_DNA
EMBLU00096; AAC74342.1; -; Genomic_DNA
EMBLAP009048; BAA14792.1; -; Genomic_DNA
EMBLX16698; CAA34671.1; -; Genomic_DNA
PIRE93746; TSECA; .
RefSeqNP_415776.1; NC_000913.2; .
PDB1V7Y; X-ray; 2.50 A; A/B=1-268
PDB1WQ5; X-ray; 2.30 A; A/B=1-268
PDB1XC4; X-ray; 2.80 A; A/B=1-268
PDB1XCF; X-ray; 1.80 A; A/B=1-268
PDBsum1V7Y; -; .
PDBsum1WQ5; -; .
PDBsum1XC4; -; .
PDBsum1XCF; -; .
ProteinModelPortalP0A877; -; .
SMRP0A877; 1-268; .
DisProtDP00252; -; .
DIPDIP-35957N; -; .
IntActP0A877; 5; .
SWISS-2DPAGEP0A877; -; .
2DBase-EcoliP0A877; -; .
ECO2DBASEE025.5; 6TH EDITION; .
EnsemblBacteriaEBESCT00000004452; EBESCP00000004452; EBESCG00000003632; .
EnsemblBacteriaEBESCT00000004453; EBESCP00000004453; EBESCG00000003632; .
EnsemblBacteriaEBESCT00000014719; EBESCP00000014010; EBESCG00000013780; .
GeneID946204; -; .
GenomeReviewsAP009048_GR; JW1252; .
GenomeReviewsU00096_GR; b1260; .
KEGGecj:JW1252; -; .
KEGGeco:b1260; -; .
EchoBASEEB1017; -; .
EcoGeneEG11024; trpA; .
eggNOGCOG0159; -; .
GeneTreeEBGT00050000009400; -; .
HOGENOMHBG391690; -; .
OMANPIYIYG; -; .
ProtClustDBPRK13111; -; .
BioCycEcoCyc:TRYPSYN-APROTEIN; -; .
BioCycMetaCyc:TRYPSYN-APROTEIN; -; .
GenevestigatorP0A877; -; .
GOGO:0005737; C:cytoplasm; IDA:EcoliWiki; .
GOGO:0043199; F:sulfate binding; IPI:EcoliWiki; .
GOGO:0004834; F:tryptophan synthase activity; IEA:EC; .
HAMAPMF_00131; Trp_synth_alpha; 1; -
InterProIPR013785; Aldolase_TIM; .
InterProIPR011060; RibuloseP-bd_barrel; .
InterProIPR018204; Trp_synthase_alpha_AS; .
InterProIPR002028; Trp_synthase_suA; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
PfamPF00290; Trp_syntA; 1; .
SUPFAMSSF51366; RibP_bind_barrel; 1; .
TIGRFAMsTIGR00262; TrpA; 1; .
PROSITEPS00167; TRP_SYNTHASE_ALPHA; 1; .



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