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SWISS-2DPAGE 
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Protein list for SWISS-2DPAGE HEPG2_HUMAN 
HepG2  {Homo sapiens (Human)}
tissue: Hepatoblastoma


(map ID: 9786996f-b534-48ea-bac9-91001d1b0779)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for hepg2_human   98 identified spots / 46 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
- Unknown protein from 2D-PAGE of liver tissue (Spot 106) (Fragment) P31929
(ULAD_HUMAN)
2D-0001WZ
2D-0001WX
2D-0001WW
6.42
6.32
6.31
149'015
149'015
149'015
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
- Unknown protein from 2D-PAGE of liver tissue (Spot 118) (Fragment) P31933
(ULAG_HUMAN)
2D-00035U
2D-00035X
5.49
5.65
37'841
37'761
{Gm}
{Gm}
-
-
1
1
- Unknown protein from 2D-PAGE of liver tissue (Spot 106) (Fragment) P31929
(ULAD_HUMAN)
2D-0001WY
2D-0001X2
2D-0001X3
6.37
6.47
6.52
149'015
148'004
148'004
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
ACADS RecName: Full=Short-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=SCAD; EC=1.3.8.1; AltName: Full=Butyryl-CoA dehydrogenase; Flags: Precursor; P16219
(ACADS_HUMAN)
2D-00034Y
6.50
38'000
{Gm}
-
1
ACTB RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed; P60709
(ACTB_HUMAN)
2D-00030B
2D-00031Z
2D-00030Z
5.15
5.23
5.09
41'700
41'233
41'700
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
ALB RecName: Full=Serum albumin; Flags: Precursor; P02768
(ALBU_HUMAN)
2D-0002GJ
2D-0002GN
2D-0002GK
2D-0002GL
2D-0002GI
5.52
5.78
5.57
5.69
5.46
66'700
67'033
66'700
67'033
66'470
{Gm}
{Gm}
{Gm}
{Gm}
{Gm}
-
-
-
-
-
1
1
1
1
1
APOA1 RecName: Full=Apolipoprotein A-I; Short=Apo-AI; Short=ApoA-I; AltName: Full=Apolipoprotein A1; Contains: RecName: Full=Apolipoprotein A-I(1-242); Flags: Precursor; P02647
(APOA1_HUMAN)
2D-0003Q0
5.22
23'900
{Gm}
-
1
ATP5D RecName: Full=ATP synthase subunit delta, mitochondrial; AltName: Full=F-ATPase delta subunit; Flags: Precursor; P30049
(ATPD_HUMAN)
2D-0003XK
4.50
13'360
{Gm}
-
1
ATP5J RecName: Full=ATP synthase-coupling factor 6, mitochondrial; Short=ATPase subunit F6; Flags: Precursor; P18859
(ATP5J_HUMAN)
2D-00040F
5.37
9'810
{Gm}
-
1
CALR RecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor; P27797
(CALR_HUMAN)
2D-0002KE
2D-0002K8
2D-0002KB
4.42
4.36
4.40
58'700
59'311
58'700
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
CAT RecName: Full=Catalase; EC=1.11.1.6; P04040
(CATA_HUMAN)
2D-0002LV
2D-0002LR
2D-0002LO
2D-0002LS
6.88
6.92
6.96
7.00
58'000
58'087
58'000
58'000
{Gm}
{Gm}
{Gm}
{Gm}
-
-
-
-
2
2
2
2
CDK1 RecName: Full=Cyclin-dependent kinase 1; Short=CDK1; EC=2.7.11.22; EC=2.7.11.23; AltName: Full=Cell division control protein 2 homolog; AltName: Full=Cell division protein kinase 1; AltName: Full=p34 protein kinase; P06493
(CDK1_HUMAN)
2D-0012IT
2D-0012IV
2D-0012IU
2D-0012IW
7.04
6.92
8.09
7.37
31'853
31'719
31'853
31'322
{Im}
{Im}
{Im}
{Im}
-
-
-
-
3
3
3
3
CDKN1A RecName: Full=Cyclin-dependent kinase inhibitor 1; AltName: Full=CDK-interacting protein 1; AltName: Full=Melanoma differentiation-associated protein 6; Short=MDA-6; AltName: Full=p21; P38936
(CDN1A_HUMAN)
2D-0012J3
2D-0012J4
7.92
7.02
19'736
19'432
{Im}
{Im}
-
-
3
3
CDKN1B RecName: Full=Cyclin-dependent kinase inhibitor 1B; AltName: Full=Cyclin-dependent kinase inhibitor p27; AltName: Full=p27Kip1; P46527
(CDN1B_HUMAN)
2D-0012IX
2D-0012IZ
2D-0012IY
6.56
6.72
6.96
25'143
24'908
25'143
{Im}
{Im}
{Im}
-
-
-
3
3
3
CMPK1 RecName: Full=UMP-CMP kinase; EC=2.7.4.14; AltName: Full=Cytidine monophosphate kinase; AltName: Full=Cytidylate kinase; AltName: Full=Deoxycytidylate kinase; AltName: Full=Uridine monophosphate kinase; AltName: Full=Uridine monophosphate/cytidine monophosphate kinase; Short=UMP/CMP kinase; Short=UMP/CMPK; P30085
(KCY_HUMAN)
2D-0003RI
5.41
21'859
{Gm}
-
1
COX5A RecName: Full=Cytochrome c oxidase subunit 5A, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide Va; Flags: Precursor; P20674
(COX5A_HUMAN)
2D-0003YO
4.93
11'646
{Gm}
-
1
COX6A1 RecName: Full=Cytochrome c oxidase subunit 6A1, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide VIa-liver; AltName: Full=Cytochrome c oxidase subunit VIA-liver; Short=COX VIa-L; Flags: Precursor; P12074
(CX6A1_HUMAN)
2D-0003Z4
6.75
11'189
{Gm}
-
1
CTSD RecName: Full=Cathepsin D; EC=3.4.23.5; Contains: RecName: Full=Cathepsin D light chain; Contains: RecName: Full=Cathepsin D heavy chain; Flags: Precursor; P07339
(CATD_HUMAN)
2D-0003JH
5.51
27'819
{Gm}
-
1
EEF1B2 RecName: Full=Elongation factor 1-beta; Short=EF-1-beta; P24534
(EF1B_HUMAN)
2D-0003I4
4.51
28'602
{Gm}
-
1
ERP29 RecName: Full=Endoplasmic reticulum resident protein 29; Short=ERp29; AltName: Full=Endoplasmic reticulum resident protein 28; Short=ERp28; AltName: Full=Endoplasmic reticulum resident protein 31; Short=ERp31; Flags: Precursor; P30040
(ERP29_HUMAN)
2D-0003LA
5.67
26'500
{Gm}
-
1
FABP1 RecName: Full=Fatty acid-binding protein, liver; AltName: Full=Fatty acid-binding protein 1; AltName: Full=Liver-type fatty acid-binding protein; Short=L-FABP; P07148
(FABPL_HUMAN)
2D-0003ZJ
2D-0003ZQ
2D-0003ZN
5.55
5.30
6.09
10'689
10'628
10'874
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
GLUD1 RecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor; P00367
(DHE3_HUMAN)
2D-0002SX
2D-0002TB
2D-0002TD
6.90
6.97
7.34
50'151
50'011
49'872
{Mi,Gm}
{Mi,Gm}
{Mi,Gm}
-
-
-
1
1
1
GSTP1 RecName: Full=Glutathione S-transferase P; EC=2.5.1.18; AltName: Full=GST class-pi; AltName: Full=GSTP1-1; P09211
(GSTP1_HUMAN)
2D-0003Q9
5.43
23'319
{Mi,Gm}
-
1
HBB RecName: Full=Hemoglobin subunit beta; AltName: Full=Beta-globin; AltName: Full=Hemoglobin beta chain; Contains: RecName: Full=LVV-hemorphin-7; P68871
(HBB_HUMAN)
2D-0003Y8
2D-0003Y9
6.92
6.94
12'262
12'332
{Gm}
{Gm}
-
-
1
1
HPGD RecName: Full=15-hydroxyprostaglandin dehydrogenase [NAD+]; Short=15-PGDH; EC=1.1.1.141; AltName: Full=Prostaglandin dehydrogenase 1; P15428
(PGDH_HUMAN)
2D-0003NH
5.60
25'475
{Mi,Gm}
-
1
HSPD1 RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=HuCHA60; AltName: Full=Mitochondrial matrix protein P1; AltName: Full=P60 lymphocyte protein; Flags: Precursor; P10809
(CH60_HUMAN)
2D-000004
2D-000006
2D-000005
2D-000008
2D-000007
5.04
5.14
5.09
5.24
5.19
58'262
57'838
58'000
57'516
57'516
{Gm}
{Gm}
{Gm}
{Gm}
{Gm}
-
-
-
-
-
1
1
1
1
1
MAP3K1 RecName: Full=Mitogen-activated protein kinase kinase kinase 1; EC=2.7.11.25; AltName: Full=MAPK/ERK kinase kinase 1; Short=MEK kinase 1; Short=MEKK 1; Q13233
(M3K1_HUMAN)
2D-0012IN
2D-0012IO
2D-0012IP
2D-0012IQ
6.50
6.79
6.90
6.93
45'349
45'349
45'096
44'844
{Im}
{Im}
{Im}
{Im}
-
-
-
-
3
3
3
3
MYC RecName: Full=Myc proto-oncogene protein; AltName: Full=Class E basic helix-loop-helix protein 39; Short=bHLHe39; AltName: Full=Proto-oncogene c-Myc; AltName: Full=Transcription factor p64; P01106
(MYC_HUMAN)
2D-0012IR
5.12
44'719
{Im}
-
3
P4HB RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor; P07237
(PDIA1_HUMAN)
2D-0002NN
2D-0002NW
2D-0002NR
2D-0002NM
4.74
4.79
4.68
4.71
55'308
55'308
55'462
55'308
{Gm}
{Gm}
{Gm}
{Gm}
-
-
-
-
1
1
1
1
PBLD RecName: Full=Phenazine biosynthesis-like domain-containing protein; EC=5.1.-.-; AltName: Full=MAWD-binding protein; AltName: Full=Unknown protein 32 from 2D-page of liver tissue; P30039
(PBLD_HUMAN)
2D-0003HO
6.30
29'069
{Gm}
-
1
PCNA RecName: Full=Proliferating cell nuclear antigen; Short=PCNA; AltName: Full=Cyclin; P12004
(PCNA_HUMAN)
2D-0012IS
4.62
35'905
{Im}
-
3
PDIA3 RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor; P30101
(PDIA3_HUMAN)
2D-0002Q8
2D-0002Q2
2D-0002Q6
2D-0002Q0
5.57
5.51
5.37
5.43
52'740
52'740
53'036
53'036
{Gm}
{Gm}
{Gm}
{Gm}
-
-
-
-
1
1
1
1
PEBP1 RecName: Full=Phosphatidylethanolamine-binding protein 1; Short=PEBP-1; AltName: Full=HCNPpp; AltName: Full=Neuropolypeptide h3; AltName: Full=Prostatic-binding protein; AltName: Full=Raf kinase inhibitor protein; Short=RKIP; Contains: RecName: Full=Hippocampal cholinergic neurostimulating peptide; Short=HCNP; P30086
(PEBP1_HUMAN)
2D-0003S5
7.07
20'738
{Gm}
-
1
PRDX3 RecName: Full=Thioredoxin-dependent peroxide reductase, mitochondrial; EC=1.11.1.15; AltName: Full=Antioxidant protein 1; Short=AOP-1; AltName: Full=HBC189; AltName: Full=Peroxiredoxin III; Short=Prx-III; AltName: Full=Peroxiredoxin-3; AltName: Full=Protein MER5 homolog; Flags: Precursor; P30048
(PRDX3_HUMAN)
2D-0003PK
6.02
24'216
{Gm}
-
1
PRDX6 RecName: Full=Peroxiredoxin-6; EC=1.11.1.15; AltName: Full=1-Cys peroxiredoxin; Short=1-Cys PRX; AltName: Full=24 kDa protein; AltName: Full=Acidic calcium-independent phospholipase A2; Short=aiPLA2; EC=3.1.1.-; AltName: Full=Antioxidant protein 2; AltName: Full=Liver 2D page spot 40; AltName: Full=Non-selenium glutathione peroxidase; Short=NSGPx; EC=1.11.1.9; AltName: Full=Red blood cells page spot 12; P30041
(PRDX6_HUMAN)
2D-0003M9
6.28
25'861
{Gm}
-
1
RRAS RecName: Full=Ras-related protein R-Ras; AltName: Full=p23; Flags: Precursor; P10301
(RRAS_HUMAN)
2D-0012J1
2D-0012J0
2D-0012J2
4.88
4.81
4.93
20'867
21'258
20'610
{Im}
{Im}
{Im}
-
-
-
3
3
3
SPTB RecName: Full=Spectrin beta chain, erythrocyte; AltName: Full=Beta-I spectrin; P11277
(SPTB1_HUMAN)
2D-0003N6
5.56
25'571
{Gm}
-
1
TF RecName: Full=Serotransferrin; Short=Transferrin; AltName: Full=Beta-1 metal-binding globulin; AltName: Full=Siderophilin; Flags: Precursor; P02787
(TRFE_HUMAN)
2D-00040O
2D-0003ZJ
2D-00040M
5.74
5.55
5.72
9'588
10'689
9'588
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
TP53 RecName: Full=Cellular tumor antigen p53; AltName: Full=Antigen NY-CO-13; AltName: Full=Phosphoprotein p53; AltName: Full=Tumor suppressor p53; P04637
(P53_HUMAN)
2D-0012IK
2D-0012IG
2D-0012ID
2D-0012IM
2D-0012IE
2D-0012IJ
2D-0012II
2D-0012IL
2D-0012IH
2D-0012IF
6.35
6.73
6.31
6.92
6.34
6.72
6.49
6.86
6.84
6.61
50'715
53'184
53'782
50'433
53'483
51'143
51'143
50'715
53'036
53'333
{Im}
{Im}
{Im}
{Im}
{Im}
{Im}
{Im}
{Im}
{Im}
{Im}
-
-
-
-
-
-
-
-
-
-
3
3
3
3
3
3
3
3
3
3
TPI1 RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P60174
(TPIS_HUMAN)
2D-0003MP
6.45
25'716
{Gm}
-
1
TPT1 RecName: Full=Translationally-controlled tumor protein; Short=TCTP; AltName: Full=Fortilin; AltName: Full=Histamine-releasing factor; Short=HRF; AltName: Full=p23; P13693
(TCTP_HUMAN)
2D-0003RC
4.86
22'200
{Gm}
-
1
TTR RecName: Full=Transthyretin; AltName: Full=ATTR; AltName: Full=Prealbumin; AltName: Full=TBPA; Flags: Precursor; P02766
(TTHY_HUMAN)
2D-0003WU
5.42
13'952
{Gm}
Positional variants
1, 4
TUBB2C RecName: Full=Tubulin beta-2C chain; AltName: Full=Tubulin beta-2 chain; P68371
(TBB2C_HUMAN)
2D-0003F8
5.32
30'800
{Gm}
-
1
TXN RecName: Full=Thioredoxin; Short=Trx; AltName: Full=ATL-derived factor; Short=ADF; AltName: Full=Surface-associated sulphydryl protein; Short=SASP; P10599
(THIO_HUMAN)
2D-0003ZB
4.92
10'936
{Gm}
-
1
UBC RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor; P0CG48
(UBC_HUMAN)
2D-00040Z
6.89
8'800
{Mi,Gm}
-
1
UQCRC1 RecName: Full=Cytochrome b-c1 complex subunit 1, mitochondrial; AltName: Full=Complex III subunit 1; AltName: Full=Core protein I; AltName: Full=Ubiquinol-cytochrome-c reductase complex core protein 1; Flags: Precursor; P31930
(QCR1_HUMAN)
2D-0002XA
5.43
45'731
{Gm}
-
1
Mapping legends: { Mi } => 'Microsequencing', { Gm } => 'Gel matching', { Im } => 'Immunobloting'



Cited references

# PubMed/Medline Authors Location
[1] 7498157 Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.; Electrophoresis 16(1):1131-1151(1995).
''''''Inside SWISS-2DPAGE database'';'';'';
[2] 109131 Edwards J., Anderson N.G., Nance S.L., Anderson N.L.; Blood 53(1):1121-1132(1979).
''''''Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins'';'';'';
[3] 9150953 Sanchez J.-C., Wirth P., Jaccoud S., Appel R.D., Sarto C., Wilkins M.R., Hochstrasser D.F.; Electrophoresis 18(1):638-641(1997).
''''''Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots'';'';'';
[4] 1286669 Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.; Electrophoresis 13(1):992-1001(1992).
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';'';


Gel experimental information

Information Type Data
Map name HEPG2_HUMAN {HepG2}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
Strain Comment [species] HepG2 [Homo sapiens (Human) / TaxID:9606]
Tissue (Swiss-Prot definition) Hepatoblastoma
pI start: 3.50 - end: 10.00
Mw start: 10000 - end: 200000
Number of detected spots 2862
Number of identified spots 98
Number of identified proteins 45
Reference Bibliographic reference
Graphical interface
[Click for graphical interface]


SWISS-2DPAGE image

SWISS-2DPAGE (search Map)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server